Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model
Abstract
Functional cartilaginous constructs for scientific research and eventual tissue repair were cultivated in bioreactors starting from chondrocytes immobilized on polymeric scaffolds. The scaffolds gradually degraded as the cells regenerated tissue matrix consisting of glycosaminoglycan (GAG) and type II collagen. To facilitate data interpretation and optimize cultivation conditions, a mathematical model was developed which yields the concentrations of oxygen and GAG as functions of time and position in growing tissue. Calculated GAG concentrations were qualitatively and quantitatively consistent with profiles measured via high-resolution image processing of tissue samples cultured at two different oxygen tensions for various periods of time.
Source:
AICHE Journal, 2000, 46, 9, 1860-1871Publisher:
- Wiley-Blackwell, Malden
DOI: 10.1002/aic.690460914
ISSN: 0001-1541
WoS: 000089382200013
Scopus: 2-s2.0-0034282186
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Obradović, Bojana AU - Meldon, JH AU - Freed, LE AU - Vunjak-Novaković, Gordana PY - 2000 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/302 AB - Functional cartilaginous constructs for scientific research and eventual tissue repair were cultivated in bioreactors starting from chondrocytes immobilized on polymeric scaffolds. The scaffolds gradually degraded as the cells regenerated tissue matrix consisting of glycosaminoglycan (GAG) and type II collagen. To facilitate data interpretation and optimize cultivation conditions, a mathematical model was developed which yields the concentrations of oxygen and GAG as functions of time and position in growing tissue. Calculated GAG concentrations were qualitatively and quantitatively consistent with profiles measured via high-resolution image processing of tissue samples cultured at two different oxygen tensions for various periods of time. PB - Wiley-Blackwell, Malden T2 - AICHE Journal T1 - Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model EP - 1871 IS - 9 SP - 1860 VL - 46 DO - 10.1002/aic.690460914 ER -
@article{ author = "Obradović, Bojana and Meldon, JH and Freed, LE and Vunjak-Novaković, Gordana", year = "2000", abstract = "Functional cartilaginous constructs for scientific research and eventual tissue repair were cultivated in bioreactors starting from chondrocytes immobilized on polymeric scaffolds. The scaffolds gradually degraded as the cells regenerated tissue matrix consisting of glycosaminoglycan (GAG) and type II collagen. To facilitate data interpretation and optimize cultivation conditions, a mathematical model was developed which yields the concentrations of oxygen and GAG as functions of time and position in growing tissue. Calculated GAG concentrations were qualitatively and quantitatively consistent with profiles measured via high-resolution image processing of tissue samples cultured at two different oxygen tensions for various periods of time.", publisher = "Wiley-Blackwell, Malden", journal = "AICHE Journal", title = "Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model", pages = "1871-1860", number = "9", volume = "46", doi = "10.1002/aic.690460914" }
Obradović, B., Meldon, J., Freed, L.,& Vunjak-Novaković, G.. (2000). Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model. in AICHE Journal Wiley-Blackwell, Malden., 46(9), 1860-1871. https://doi.org/10.1002/aic.690460914
Obradović B, Meldon J, Freed L, Vunjak-Novaković G. Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model. in AICHE Journal. 2000;46(9):1860-1871. doi:10.1002/aic.690460914 .
Obradović, Bojana, Meldon, JH, Freed, LE, Vunjak-Novaković, Gordana, "Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model" in AICHE Journal, 46, no. 9 (2000):1860-1871, https://doi.org/10.1002/aic.690460914 . .