Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose
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2016
Authors
Jugović, BranimirGrgur, Branimir
Antov, Mirjana
Knežević-Jugović, Zorica
Stevanović, Jasmina
Gvozdenović, Milica
Article (Published version)
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Show full item recordAbstract
Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
Keywords:
conducting polymers / electrochemistry / glucose oxidase / material testing / sensorsSource:
International Journal of Electrochemical Science, 2016, 11, 2, 1152-1161Publisher:
- Electrochemical Science Group, Beograd
Funding / projects:
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Jugović, Branimir AU - Grgur, Branimir AU - Antov, Mirjana AU - Knežević-Jugović, Zorica AU - Stevanović, Jasmina AU - Gvozdenović, Milica PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3184 AB - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix. PB - Electrochemical Science Group, Beograd T2 - International Journal of Electrochemical Science T1 - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose EP - 1161 IS - 2 SP - 1152 VL - 11 UR - https://hdl.handle.net/21.15107/rcub_technorep_3184 ER -
@article{ author = "Jugović, Branimir and Grgur, Branimir and Antov, Mirjana and Knežević-Jugović, Zorica and Stevanović, Jasmina and Gvozdenović, Milica", year = "2016", abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.", publisher = "Electrochemical Science Group, Beograd", journal = "International Journal of Electrochemical Science", title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose", pages = "1161-1152", number = "2", volume = "11", url = "https://hdl.handle.net/21.15107/rcub_technorep_3184" }
Jugović, B., Grgur, B., Antov, M., Knežević-Jugović, Z., Stevanović, J.,& Gvozdenović, M.. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science Electrochemical Science Group, Beograd., 11(2), 1152-1161. https://hdl.handle.net/21.15107/rcub_technorep_3184
Jugović B, Grgur B, Antov M, Knežević-Jugović Z, Stevanović J, Gvozdenović M. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science. 2016;11(2):1152-1161. https://hdl.handle.net/21.15107/rcub_technorep_3184 .
Jugović, Branimir, Grgur, Branimir, Antov, Mirjana, Knežević-Jugović, Zorica, Stevanović, Jasmina, Gvozdenović, Milica, "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" in International Journal of Electrochemical Science, 11, no. 2 (2016):1152-1161, https://hdl.handle.net/21.15107/rcub_technorep_3184 .