Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids
Authorized Users Only
2016
Authors
Pajović, Jelena D.Dojčilović, Radovan
Božanić, Dušan K.
Vodnik, Vesna
Dimitrijević-Branković, Suzana
Kascakova, Slavka
Refregiers, Matthieu
Markelić, Milica
Đoković, Vladimir
Article (Published version)
Metadata
Show full item recordAbstract
Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.
Keywords:
Gold nanoparticles / Riboflavin / Functionalization / Candida albicans / Fluorescence imagingSource:
Optical and Quantum Electronics, 2016, 48, 6Publisher:
- Springer, Dordrecht
Funding / projects:
- [20120810]
- [20131218]
- Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites (RS-172056)
- Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion (RS-45020)
- Investigation of the effect of parameters of synthesis and processing on dielectric, optical and magnetic properties, both bulk and surface of crystal and polymeric systems (RS-171029)
DOI: 10.1007/s11082-016-0578-y
ISSN: 0306-8919
WoS: 000377387300004
Scopus: 2-s2.0-84966440446
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Pajović, Jelena D. AU - Dojčilović, Radovan AU - Božanić, Dušan K. AU - Vodnik, Vesna AU - Dimitrijević-Branković, Suzana AU - Kascakova, Slavka AU - Refregiers, Matthieu AU - Markelić, Milica AU - Đoković, Vladimir PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3256 AB - Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology. PB - Springer, Dordrecht T2 - Optical and Quantum Electronics T1 - Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids IS - 6 VL - 48 DO - 10.1007/s11082-016-0578-y ER -
@article{ author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana and Kascakova, Slavka and Refregiers, Matthieu and Markelić, Milica and Đoković, Vladimir", year = "2016", abstract = "Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.", publisher = "Springer, Dordrecht", journal = "Optical and Quantum Electronics", title = "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids", number = "6", volume = "48", doi = "10.1007/s11082-016-0578-y" }
Pajović, J. D., Dojčilović, R., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S., Kascakova, S., Refregiers, M., Markelić, M.,& Đoković, V.. (2016). Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics Springer, Dordrecht., 48(6). https://doi.org/10.1007/s11082-016-0578-y
Pajović JD, Dojčilović R, Božanić DK, Vodnik V, Dimitrijević-Branković S, Kascakova S, Refregiers M, Markelić M, Đoković V. Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics. 2016;48(6). doi:10.1007/s11082-016-0578-y .
Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana, Kascakova, Slavka, Refregiers, Matthieu, Markelić, Milica, Đoković, Vladimir, "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids" in Optical and Quantum Electronics, 48, no. 6 (2016), https://doi.org/10.1007/s11082-016-0578-y . .