Epoxy-silanization - tool for improvement of silica nanoparticles as support for lipase immobilization with respect to esterification activity
Само за регистроване кориснике
2016
Аутори
Banjanac, KatarinaMihailović, Mladen
Prlainović, Nevena
Ćorović, Marija
Simović, Milica
Marinković, Aleksandar
Bezbradica, Dejan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
BACKGROUND: In this study, in order to obtain the best support for the immobilization of lipase from Candida rugosa (CRL), the procedure for chemical modification of fumed silica nanoparticles (FNS) with (3-Glycidyloxypropyl) trimethoxysilane (GOPTMS) was optimized by varying the amount of GOPTMS used and the duration of support modification. To evaluate the prospects of chemical modification of FNS surface on the immobilization process, the activity and thermal stability of lipase immobilized on GFNS was compared with lipase immobilized on FNS and cyanuric chloride functionalized amino silica (CCAFNS). RESULTS: The epoxy functionalized silica nanoparticles (GFNS) with 400-500 mu mol of introduced epoxy groups per g of support were the most efficient, since immobilized preparations in 1 mol L-1 buffer had the highest activity of approximately 1495 IU g(-1) and a loading capacity of 294 mg g(-1). Lipase immobilized on GFNS exhibited 1.5-fold higher hydrolytic activity and 2-fold higher ...esterification activity in the synthesis of aroma esters than lipase immobilized on CCAFNS, while its thermal stability was similar to CCAFNS preparations. CONCLUSION: These results indicated that the type of modification of the FNS surface has an influence on the specificity of the immobilized lipase, since lipase immobilized on GFNS showed improved properties for ester synthesis which is a promising area for immobilized lipase application.
Кључне речи:
biotechnology / biocatalysis / lipase / immobilizationИзвор:
Journal of Chemical Technology and Biotechnology, 2016, 91, 10, 2654-2663Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Проучавање синтезе, структуре и активности органских једињења природног и синтетског порекла (RS-MESTD-Basic Research (BR or ON)-172013)
- Синтеза, развој технологија добијања и примена наноструктурних мултифункционалних материјала дефинисаних својстава (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45019)
DOI: 10.1002/jctb.4870
ISSN: 0268-2575
WoS: 000388291900012
Scopus: 2-s2.0-84953294965
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Banjanac, Katarina AU - Mihailović, Mladen AU - Prlainović, Nevena AU - Ćorović, Marija AU - Simović, Milica AU - Marinković, Aleksandar AU - Bezbradica, Dejan PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3323 AB - BACKGROUND: In this study, in order to obtain the best support for the immobilization of lipase from Candida rugosa (CRL), the procedure for chemical modification of fumed silica nanoparticles (FNS) with (3-Glycidyloxypropyl) trimethoxysilane (GOPTMS) was optimized by varying the amount of GOPTMS used and the duration of support modification. To evaluate the prospects of chemical modification of FNS surface on the immobilization process, the activity and thermal stability of lipase immobilized on GFNS was compared with lipase immobilized on FNS and cyanuric chloride functionalized amino silica (CCAFNS). RESULTS: The epoxy functionalized silica nanoparticles (GFNS) with 400-500 mu mol of introduced epoxy groups per g of support were the most efficient, since immobilized preparations in 1 mol L-1 buffer had the highest activity of approximately 1495 IU g(-1) and a loading capacity of 294 mg g(-1). Lipase immobilized on GFNS exhibited 1.5-fold higher hydrolytic activity and 2-fold higher esterification activity in the synthesis of aroma esters than lipase immobilized on CCAFNS, while its thermal stability was similar to CCAFNS preparations. CONCLUSION: These results indicated that the type of modification of the FNS surface has an influence on the specificity of the immobilized lipase, since lipase immobilized on GFNS showed improved properties for ester synthesis which is a promising area for immobilized lipase application. PB - Wiley, Hoboken T2 - Journal of Chemical Technology and Biotechnology T1 - Epoxy-silanization - tool for improvement of silica nanoparticles as support for lipase immobilization with respect to esterification activity EP - 2663 IS - 10 SP - 2654 VL - 91 DO - 10.1002/jctb.4870 ER -
@article{ author = "Banjanac, Katarina and Mihailović, Mladen and Prlainović, Nevena and Ćorović, Marija and Simović, Milica and Marinković, Aleksandar and Bezbradica, Dejan", year = "2016", abstract = "BACKGROUND: In this study, in order to obtain the best support for the immobilization of lipase from Candida rugosa (CRL), the procedure for chemical modification of fumed silica nanoparticles (FNS) with (3-Glycidyloxypropyl) trimethoxysilane (GOPTMS) was optimized by varying the amount of GOPTMS used and the duration of support modification. To evaluate the prospects of chemical modification of FNS surface on the immobilization process, the activity and thermal stability of lipase immobilized on GFNS was compared with lipase immobilized on FNS and cyanuric chloride functionalized amino silica (CCAFNS). RESULTS: The epoxy functionalized silica nanoparticles (GFNS) with 400-500 mu mol of introduced epoxy groups per g of support were the most efficient, since immobilized preparations in 1 mol L-1 buffer had the highest activity of approximately 1495 IU g(-1) and a loading capacity of 294 mg g(-1). Lipase immobilized on GFNS exhibited 1.5-fold higher hydrolytic activity and 2-fold higher esterification activity in the synthesis of aroma esters than lipase immobilized on CCAFNS, while its thermal stability was similar to CCAFNS preparations. CONCLUSION: These results indicated that the type of modification of the FNS surface has an influence on the specificity of the immobilized lipase, since lipase immobilized on GFNS showed improved properties for ester synthesis which is a promising area for immobilized lipase application.", publisher = "Wiley, Hoboken", journal = "Journal of Chemical Technology and Biotechnology", title = "Epoxy-silanization - tool for improvement of silica nanoparticles as support for lipase immobilization with respect to esterification activity", pages = "2663-2654", number = "10", volume = "91", doi = "10.1002/jctb.4870" }
Banjanac, K., Mihailović, M., Prlainović, N., Ćorović, M., Simović, M., Marinković, A.,& Bezbradica, D.. (2016). Epoxy-silanization - tool for improvement of silica nanoparticles as support for lipase immobilization with respect to esterification activity. in Journal of Chemical Technology and Biotechnology Wiley, Hoboken., 91(10), 2654-2663. https://doi.org/10.1002/jctb.4870
Banjanac K, Mihailović M, Prlainović N, Ćorović M, Simović M, Marinković A, Bezbradica D. Epoxy-silanization - tool for improvement of silica nanoparticles as support for lipase immobilization with respect to esterification activity. in Journal of Chemical Technology and Biotechnology. 2016;91(10):2654-2663. doi:10.1002/jctb.4870 .
Banjanac, Katarina, Mihailović, Mladen, Prlainović, Nevena, Ćorović, Marija, Simović, Milica, Marinković, Aleksandar, Bezbradica, Dejan, "Epoxy-silanization - tool for improvement of silica nanoparticles as support for lipase immobilization with respect to esterification activity" in Journal of Chemical Technology and Biotechnology, 91, no. 10 (2016):2654-2663, https://doi.org/10.1002/jctb.4870 . .