Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology
Abstract
Pretreatment of fenugreek (Trigonella foenum-graecum L, Fabaceae) seeds was achieved using the step of defatting and acid hydrolysis. The obtained hydro-isolate (HI) was used as initial feed for diosgenin isolation by supercritical CO2 extraction (SC-CO2). Preliminary tests performed at conditions resulting in SC-CO2 density ranging from 800 to 920 kg/m(3) indicated that at some pressure, temperature as well as consumption of supercritical fluids the optimal working conditions for diosgenin isolation could be determined. For this purpose, the following range of working conditions of SC-CO2 were tested by using Central Composite Rotatable Design (CCRD) and Response Surface Methodology (RSM): pressure from 16.6 to 33.4 MPa, temperature from 30 to 50 degrees C and consumption of SC-CO2 from 11.6 to 28.4 g(co2)/g(dm). The result of this investigation indicated that maximum yield of 0.81 mg diosgenin from 1 g of fenugreek seeds on dry basis (about 1.3% of total extract) at 24.6 MPa, 43.5 de...grees C, and 20.87 g(co2)/g(dm) could be obtained.
Keywords:
Supercritical extraction / Diosgenin / Fenugreek / RSM / OptimizationSource:
Journal of Supercritical Fluids, 2016, 107, 44-50Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
DOI: 10.1016/j.supflu.2015.08.003
ISSN: 0896-8446
WoS: 000366077100006
Scopus: 2-s2.0-84941049330
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Bogdanović, Aleksandra AU - Tadić, Vanja AU - Stamenić, Marko AU - Petrović, Slobodan AU - Skala, Dejan PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3405 AB - Pretreatment of fenugreek (Trigonella foenum-graecum L, Fabaceae) seeds was achieved using the step of defatting and acid hydrolysis. The obtained hydro-isolate (HI) was used as initial feed for diosgenin isolation by supercritical CO2 extraction (SC-CO2). Preliminary tests performed at conditions resulting in SC-CO2 density ranging from 800 to 920 kg/m(3) indicated that at some pressure, temperature as well as consumption of supercritical fluids the optimal working conditions for diosgenin isolation could be determined. For this purpose, the following range of working conditions of SC-CO2 were tested by using Central Composite Rotatable Design (CCRD) and Response Surface Methodology (RSM): pressure from 16.6 to 33.4 MPa, temperature from 30 to 50 degrees C and consumption of SC-CO2 from 11.6 to 28.4 g(co2)/g(dm). The result of this investigation indicated that maximum yield of 0.81 mg diosgenin from 1 g of fenugreek seeds on dry basis (about 1.3% of total extract) at 24.6 MPa, 43.5 degrees C, and 20.87 g(co2)/g(dm) could be obtained. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Supercritical Fluids T1 - Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology EP - 50 SP - 44 VL - 107 DO - 10.1016/j.supflu.2015.08.003 ER -
@article{ author = "Bogdanović, Aleksandra and Tadić, Vanja and Stamenić, Marko and Petrović, Slobodan and Skala, Dejan", year = "2016", abstract = "Pretreatment of fenugreek (Trigonella foenum-graecum L, Fabaceae) seeds was achieved using the step of defatting and acid hydrolysis. The obtained hydro-isolate (HI) was used as initial feed for diosgenin isolation by supercritical CO2 extraction (SC-CO2). Preliminary tests performed at conditions resulting in SC-CO2 density ranging from 800 to 920 kg/m(3) indicated that at some pressure, temperature as well as consumption of supercritical fluids the optimal working conditions for diosgenin isolation could be determined. For this purpose, the following range of working conditions of SC-CO2 were tested by using Central Composite Rotatable Design (CCRD) and Response Surface Methodology (RSM): pressure from 16.6 to 33.4 MPa, temperature from 30 to 50 degrees C and consumption of SC-CO2 from 11.6 to 28.4 g(co2)/g(dm). The result of this investigation indicated that maximum yield of 0.81 mg diosgenin from 1 g of fenugreek seeds on dry basis (about 1.3% of total extract) at 24.6 MPa, 43.5 degrees C, and 20.87 g(co2)/g(dm) could be obtained.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Supercritical Fluids", title = "Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology", pages = "50-44", volume = "107", doi = "10.1016/j.supflu.2015.08.003" }
Bogdanović, A., Tadić, V., Stamenić, M., Petrović, S.,& Skala, D.. (2016). Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology. in Journal of Supercritical Fluids Elsevier Science Bv, Amsterdam., 107, 44-50. https://doi.org/10.1016/j.supflu.2015.08.003
Bogdanović A, Tadić V, Stamenić M, Petrović S, Skala D. Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology. in Journal of Supercritical Fluids. 2016;107:44-50. doi:10.1016/j.supflu.2015.08.003 .
Bogdanović, Aleksandra, Tadić, Vanja, Stamenić, Marko, Petrović, Slobodan, Skala, Dejan, "Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology" in Journal of Supercritical Fluids, 107 (2016):44-50, https://doi.org/10.1016/j.supflu.2015.08.003 . .