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Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization
dc.creator | Buntić, Aneta | |
dc.creator | Pavlović, Marija D. | |
dc.creator | Antonović, Dušan | |
dc.creator | Šiler-Marinković, Slavica | |
dc.creator | Dimitrijević-Branković, Suzana | |
dc.date.accessioned | 2021-03-10T13:12:39Z | |
dc.date.available | 2021-03-10T13:12:39Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 2405-8440 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3414 | |
dc.description.abstract | This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 degrees C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K+ and Mg2+ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme application. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization. | en |
dc.publisher | Elsevier Sci Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Heliyon | |
dc.subject | Biotechnology | en |
dc.subject | Food science | en |
dc.subject | Materials science | en |
dc.subject | Bioengineering | en |
dc.subject | Ecology | en |
dc.title | Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.issue | 8 | |
dc.citation.other | 2(8): - | |
dc.citation.volume | 2 | |
dc.identifier.doi | 10.1016/j.heliyon.2016.e00146 | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/1323/3411.pdf | |
dc.identifier.pmid | 27626091 | |
dc.identifier.scopus | 2-s2.0-84989227454 | |
dc.identifier.wos | 000432010800002 | |
dc.type.version | publishedVersion |