Immobilization of horseradish peroxidase onto kaolin
Само за регистроване кориснике
2016
Аутори
Šekuljica, NatašaPrlainović, Nevena
Jovanović, Jelena
Stefanović, Andrea
Đokić, Veljko
Mijin, Dušan
Knežević-Jugović, Zorica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Kaolin showed as a very perspective carrier for the enzyme immobilization and it was used for the adsorption of horseradish peroxidase (HRP). The effects of the enzyme concentration and pH on the immobilization efficiency were studied in the reaction with pyrogallol and anthraquinone dye C.I. Acid Violet 109 (AV 109). In addition, Fourier transform infrared spectroscopy, scanning electron microscopy and analysis by Brunauer-EmmettTeller were performed for kaolin, thermally activated kaolin and the immobilized enzyme. It has been shown that 0.1 IU of HRP-kaolin decolorized 87 % of dye solution, under the optimal conditions (pH 5.0, temperature 24 degrees C, dye concentration 40 mg/L and 0.2 mM of H2O2) within 40 min. The immobilized HRP decolorization follows the Ping Pong Bi-Bi mechanism with dead-end inhibition by the dye. The biocatalyst retained 35 +/- 0.9 % of the initial activity after seven cycles of reuse in the decolorization reaction of AV 109 under optimal conditions in a bat...ch reactor. The obtained kinetic parameters and reusability study confirmed improvement in performances of k-HRP compared to free, indicating that k-HRP has a great potential for environmental purposes.
Кључне речи:
Horseradish peroxidase / Kaolin / Adsorption / Kinetic parameters / Wastewater treatmentИзвор:
Bioprocess and Biosystems Engineering, 2016, 39, 3, 461-472Издавач:
- Springer, New York
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Проучавање синтезе, структуре и активности органских једињења природног и синтетског порекла (RS-MESTD-Basic Research (BR or ON)-172013)
DOI: 10.1007/s00449-015-1529-x
ISSN: 1615-7591
PubMed: 26747440
WoS: 000371071100010
Scopus: 2-s2.0-84960101239
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Šekuljica, Nataša AU - Prlainović, Nevena AU - Jovanović, Jelena AU - Stefanović, Andrea AU - Đokić, Veljko AU - Mijin, Dušan AU - Knežević-Jugović, Zorica PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3466 AB - Kaolin showed as a very perspective carrier for the enzyme immobilization and it was used for the adsorption of horseradish peroxidase (HRP). The effects of the enzyme concentration and pH on the immobilization efficiency were studied in the reaction with pyrogallol and anthraquinone dye C.I. Acid Violet 109 (AV 109). In addition, Fourier transform infrared spectroscopy, scanning electron microscopy and analysis by Brunauer-EmmettTeller were performed for kaolin, thermally activated kaolin and the immobilized enzyme. It has been shown that 0.1 IU of HRP-kaolin decolorized 87 % of dye solution, under the optimal conditions (pH 5.0, temperature 24 degrees C, dye concentration 40 mg/L and 0.2 mM of H2O2) within 40 min. The immobilized HRP decolorization follows the Ping Pong Bi-Bi mechanism with dead-end inhibition by the dye. The biocatalyst retained 35 +/- 0.9 % of the initial activity after seven cycles of reuse in the decolorization reaction of AV 109 under optimal conditions in a batch reactor. The obtained kinetic parameters and reusability study confirmed improvement in performances of k-HRP compared to free, indicating that k-HRP has a great potential for environmental purposes. PB - Springer, New York T2 - Bioprocess and Biosystems Engineering T1 - Immobilization of horseradish peroxidase onto kaolin EP - 472 IS - 3 SP - 461 VL - 39 DO - 10.1007/s00449-015-1529-x ER -
@article{ author = "Šekuljica, Nataša and Prlainović, Nevena and Jovanović, Jelena and Stefanović, Andrea and Đokić, Veljko and Mijin, Dušan and Knežević-Jugović, Zorica", year = "2016", abstract = "Kaolin showed as a very perspective carrier for the enzyme immobilization and it was used for the adsorption of horseradish peroxidase (HRP). The effects of the enzyme concentration and pH on the immobilization efficiency were studied in the reaction with pyrogallol and anthraquinone dye C.I. Acid Violet 109 (AV 109). In addition, Fourier transform infrared spectroscopy, scanning electron microscopy and analysis by Brunauer-EmmettTeller were performed for kaolin, thermally activated kaolin and the immobilized enzyme. It has been shown that 0.1 IU of HRP-kaolin decolorized 87 % of dye solution, under the optimal conditions (pH 5.0, temperature 24 degrees C, dye concentration 40 mg/L and 0.2 mM of H2O2) within 40 min. The immobilized HRP decolorization follows the Ping Pong Bi-Bi mechanism with dead-end inhibition by the dye. The biocatalyst retained 35 +/- 0.9 % of the initial activity after seven cycles of reuse in the decolorization reaction of AV 109 under optimal conditions in a batch reactor. The obtained kinetic parameters and reusability study confirmed improvement in performances of k-HRP compared to free, indicating that k-HRP has a great potential for environmental purposes.", publisher = "Springer, New York", journal = "Bioprocess and Biosystems Engineering", title = "Immobilization of horseradish peroxidase onto kaolin", pages = "472-461", number = "3", volume = "39", doi = "10.1007/s00449-015-1529-x" }
Šekuljica, N., Prlainović, N., Jovanović, J., Stefanović, A., Đokić, V., Mijin, D.,& Knežević-Jugović, Z.. (2016). Immobilization of horseradish peroxidase onto kaolin. in Bioprocess and Biosystems Engineering Springer, New York., 39(3), 461-472. https://doi.org/10.1007/s00449-015-1529-x
Šekuljica N, Prlainović N, Jovanović J, Stefanović A, Đokić V, Mijin D, Knežević-Jugović Z. Immobilization of horseradish peroxidase onto kaolin. in Bioprocess and Biosystems Engineering. 2016;39(3):461-472. doi:10.1007/s00449-015-1529-x .
Šekuljica, Nataša, Prlainović, Nevena, Jovanović, Jelena, Stefanović, Andrea, Đokić, Veljko, Mijin, Dušan, Knežević-Jugović, Zorica, "Immobilization of horseradish peroxidase onto kaolin" in Bioprocess and Biosystems Engineering, 39, no. 3 (2016):461-472, https://doi.org/10.1007/s00449-015-1529-x . .