An assessment of the genetic integrity of micropropagated raspberry and blackberry plants
Samo za registrovane korisnike
2017
Autori
Vujović, TatjanaRužić, Đurđina
Cerović, Radosav
Leposavić, Aleksandar
Karaklajić-Stajić, Zaklina
Mitrović, Olga
Zurawicz, Edward
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In vitro clonal propagation of small fruit species has been one of the most broadly exploited approaches in biotechnology. However, the most critical aspect of this propagation technology has been the maintenance of genetic fidelity of tissue culture-raised plants. In the present study, three techniques were deployed in the assessment of genetic fidelity of micropropagated plants (TC plants) of blackberry cultivar Cacanska Bestrna' and raspberry cultivar 'Meeker' in the second year after planting in the open field, in comparison to plants originating from the standard planting material (ST plants). Flow cytometry analysis deployed to estimate the DNA ploidy level and relative nuclear DNA content in TC and ST plants revealed no significant differences in nuclear DNA content between plants of different origin. Chromosome counting in root/shoot tip meristems also showed a normal tetraploid chromosome number (2n = 4x = 28) in TC blackberry plants and a normal diploid chromosome number (2n ...= 2x = 14) in TC raspberry plants. Polyacrylamide gel electrophoresis of peroxidase (POX) extracted from the leaf tissue revealed no differences in POX profiles between TC and ST plants either. The results obtained in this study verify the suitability of micropropagation by axillary branching for commercial exploitation in blackberry and raspberry.
Ključne reči:
In vitro propagation / Genetic fidelity / Flow cytometry / Chromosome number / Isoperoxidase / Rubus spIzvor:
Scientia Horticulturae, 2017, 225, 454-461Izdavač:
- Elsevier Science Bv, Amsterdam
Finansiranje / projekti:
- EUBERRY - The sustainable improvement of European berry production, quality and nutritional value in a changing environment: Strawberries, Currants, Blackberries, Blueberries and Raspberries (EU-265942)
- Stvaranje i očuvanje genetičkog potencijala kontinentalnih vrsta voćaka (RS-31064)
DOI: 10.1016/j.scienta.2017.07.020
ISSN: 0304-4238
WoS: 000412606900059
Scopus: 2-s2.0-85026499929
Institucija/grupa
Inovacioni centarTY - JOUR AU - Vujović, Tatjana AU - Ružić, Đurđina AU - Cerović, Radosav AU - Leposavić, Aleksandar AU - Karaklajić-Stajić, Zaklina AU - Mitrović, Olga AU - Zurawicz, Edward PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3554 AB - In vitro clonal propagation of small fruit species has been one of the most broadly exploited approaches in biotechnology. However, the most critical aspect of this propagation technology has been the maintenance of genetic fidelity of tissue culture-raised plants. In the present study, three techniques were deployed in the assessment of genetic fidelity of micropropagated plants (TC plants) of blackberry cultivar Cacanska Bestrna' and raspberry cultivar 'Meeker' in the second year after planting in the open field, in comparison to plants originating from the standard planting material (ST plants). Flow cytometry analysis deployed to estimate the DNA ploidy level and relative nuclear DNA content in TC and ST plants revealed no significant differences in nuclear DNA content between plants of different origin. Chromosome counting in root/shoot tip meristems also showed a normal tetraploid chromosome number (2n = 4x = 28) in TC blackberry plants and a normal diploid chromosome number (2n = 2x = 14) in TC raspberry plants. Polyacrylamide gel electrophoresis of peroxidase (POX) extracted from the leaf tissue revealed no differences in POX profiles between TC and ST plants either. The results obtained in this study verify the suitability of micropropagation by axillary branching for commercial exploitation in blackberry and raspberry. PB - Elsevier Science Bv, Amsterdam T2 - Scientia Horticulturae T1 - An assessment of the genetic integrity of micropropagated raspberry and blackberry plants EP - 461 SP - 454 VL - 225 DO - 10.1016/j.scienta.2017.07.020 ER -
@article{ author = "Vujović, Tatjana and Ružić, Đurđina and Cerović, Radosav and Leposavić, Aleksandar and Karaklajić-Stajić, Zaklina and Mitrović, Olga and Zurawicz, Edward", year = "2017", abstract = "In vitro clonal propagation of small fruit species has been one of the most broadly exploited approaches in biotechnology. However, the most critical aspect of this propagation technology has been the maintenance of genetic fidelity of tissue culture-raised plants. In the present study, three techniques were deployed in the assessment of genetic fidelity of micropropagated plants (TC plants) of blackberry cultivar Cacanska Bestrna' and raspberry cultivar 'Meeker' in the second year after planting in the open field, in comparison to plants originating from the standard planting material (ST plants). Flow cytometry analysis deployed to estimate the DNA ploidy level and relative nuclear DNA content in TC and ST plants revealed no significant differences in nuclear DNA content between plants of different origin. Chromosome counting in root/shoot tip meristems also showed a normal tetraploid chromosome number (2n = 4x = 28) in TC blackberry plants and a normal diploid chromosome number (2n = 2x = 14) in TC raspberry plants. Polyacrylamide gel electrophoresis of peroxidase (POX) extracted from the leaf tissue revealed no differences in POX profiles between TC and ST plants either. The results obtained in this study verify the suitability of micropropagation by axillary branching for commercial exploitation in blackberry and raspberry.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Scientia Horticulturae", title = "An assessment of the genetic integrity of micropropagated raspberry and blackberry plants", pages = "461-454", volume = "225", doi = "10.1016/j.scienta.2017.07.020" }
Vujović, T., Ružić, Đ., Cerović, R., Leposavić, A., Karaklajić-Stajić, Z., Mitrović, O.,& Zurawicz, E.. (2017). An assessment of the genetic integrity of micropropagated raspberry and blackberry plants. in Scientia Horticulturae Elsevier Science Bv, Amsterdam., 225, 454-461. https://doi.org/10.1016/j.scienta.2017.07.020
Vujović T, Ružić Đ, Cerović R, Leposavić A, Karaklajić-Stajić Z, Mitrović O, Zurawicz E. An assessment of the genetic integrity of micropropagated raspberry and blackberry plants. in Scientia Horticulturae. 2017;225:454-461. doi:10.1016/j.scienta.2017.07.020 .
Vujović, Tatjana, Ružić, Đurđina, Cerović, Radosav, Leposavić, Aleksandar, Karaklajić-Stajić, Zaklina, Mitrović, Olga, Zurawicz, Edward, "An assessment of the genetic integrity of micropropagated raspberry and blackberry plants" in Scientia Horticulturae, 225 (2017):454-461, https://doi.org/10.1016/j.scienta.2017.07.020 . .