Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate
Аутори
Miljković, MionaDavidović, Slađana
Kralj, Slavko
Šiler-Marinković, Slavica
Rajilić-Stojanović, Mirjana
Dimitrijević-Branković, Suzana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 degrees C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K2HPO4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 degrees C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca2+, Mg2+, Fe2+ and Co2+ led to a minor increase of DS activity, while the addition of Mn2+ was the most pro...minent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries.
Кључне речи:
lactic acid bacteria / Leuconostoc mesenteroides / dextransucrase / dextranИзвор:
Hemijska industrija, 2017, 71, 4, 351-360Издавач:
- Savez hemijskih inženjera, Beograd
Финансирање / пројекти:
DOI: 10.2298/HEMIND160421046M
ISSN: 0367-598X
WoS: 000413180100009
Scopus: 2-s2.0-85030211936
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Miljković, Miona AU - Davidović, Slađana AU - Kralj, Slavko AU - Šiler-Marinković, Slavica AU - Rajilić-Stojanović, Mirjana AU - Dimitrijević-Branković, Suzana PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3632 AB - The production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 degrees C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K2HPO4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 degrees C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca2+, Mg2+, Fe2+ and Co2+ led to a minor increase of DS activity, while the addition of Mn2+ was the most prominent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries. PB - Savez hemijskih inženjera, Beograd T2 - Hemijska industrija T1 - Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate EP - 360 IS - 4 SP - 351 VL - 71 DO - 10.2298/HEMIND160421046M ER -
@article{ author = "Miljković, Miona and Davidović, Slađana and Kralj, Slavko and Šiler-Marinković, Slavica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana", year = "2017", abstract = "The production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 degrees C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K2HPO4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 degrees C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca2+, Mg2+, Fe2+ and Co2+ led to a minor increase of DS activity, while the addition of Mn2+ was the most prominent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries.", publisher = "Savez hemijskih inženjera, Beograd", journal = "Hemijska industrija", title = "Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate", pages = "360-351", number = "4", volume = "71", doi = "10.2298/HEMIND160421046M" }
Miljković, M., Davidović, S., Kralj, S., Šiler-Marinković, S., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2017). Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate. in Hemijska industrija Savez hemijskih inženjera, Beograd., 71(4), 351-360. https://doi.org/10.2298/HEMIND160421046M
Miljković M, Davidović S, Kralj S, Šiler-Marinković S, Rajilić-Stojanović M, Dimitrijević-Branković S. Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate. in Hemijska industrija. 2017;71(4):351-360. doi:10.2298/HEMIND160421046M .
Miljković, Miona, Davidović, Slađana, Kralj, Slavko, Šiler-Marinković, Slavica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate" in Hemijska industrija, 71, no. 4 (2017):351-360, https://doi.org/10.2298/HEMIND160421046M . .