One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging
2018
Autori
Mančić, LidijaĐukić-Vuković, Aleksandra
Dinić, Ivana
Nikolić, Marko G.
Rabasović, Mihailo D.
Krmpot, Aleksandar
Costa, Antonio Mario Leal Martins
Marinković, Bojan A.
Mojović, Ljiljana
Milošević, Olivera
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic alpha phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (H-2(11/2), S-4(3/2) - gt (4)/(15/2)) and stronger red emission (F-4(9/2) - gt (4)/(15/2)), as a result ...of enhanced non-radiative (4)/(11/2) - gt (4)/(13/2) Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 mu g ml(-1), without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma.
Izvor:
RSC Advances, 2018, 8, 48, 27429-27437Izdavač:
- Royal Society of Chemistry
Finansiranje / projekti:
- CNPq (National Council for Scientific and Technological Development)National Council for Scientific and Technological Development (CNPq)
- Racionalni dizajn i sinteza biološki aktivnih i koordinacionih jedinjenja i funkcionalnih materijala, relevantnih u (bio)nanotehnologiji (RS-MESTD-Basic Research (BR or ON)-172035)
- Generisanje i karakterizacija nanofotonskih funkcionalnih struktura u biomedicini i informatici (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45016)
Napomena:
- Supplementary information: https://technorep.tmf.bg.ac.rs/handle/123456789/3772
Povezane informacije:
DOI: 10.1039/c8ra04178d
ISSN: 2046-2069
WoS: 000445747800050
Scopus: 2-s2.0-85051345804
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Mančić, Lidija AU - Đukić-Vuković, Aleksandra AU - Dinić, Ivana AU - Nikolić, Marko G. AU - Rabasović, Mihailo D. AU - Krmpot, Aleksandar AU - Costa, Antonio Mario Leal Martins AU - Marinković, Bojan A. AU - Mojović, Ljiljana AU - Milošević, Olivera PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3885 AB - The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic alpha phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (H-2(11/2), S-4(3/2) - gt (4)/(15/2)) and stronger red emission (F-4(9/2) - gt (4)/(15/2)), as a result of enhanced non-radiative (4)/(11/2) - gt (4)/(13/2) Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 mu g ml(-1), without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma. PB - Royal Society of Chemistry T2 - RSC Advances T1 - One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging EP - 27437 IS - 48 SP - 27429 VL - 8 DO - 10.1039/c8ra04178d ER -
@article{ author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar and Costa, Antonio Mario Leal Martins and Marinković, Bojan A. and Mojović, Ljiljana and Milošević, Olivera", year = "2018", abstract = "The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic alpha phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (H-2(11/2), S-4(3/2) - gt (4)/(15/2)) and stronger red emission (F-4(9/2) - gt (4)/(15/2)), as a result of enhanced non-radiative (4)/(11/2) - gt (4)/(13/2) Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 mu g ml(-1), without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma.", publisher = "Royal Society of Chemistry", journal = "RSC Advances", title = "One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging", pages = "27437-27429", number = "48", volume = "8", doi = "10.1039/c8ra04178d" }
Mančić, L., Đukić-Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A., Costa, A. M. L. M., Marinković, B. A., Mojović, L.,& Milošević, O.. (2018). One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. in RSC Advances Royal Society of Chemistry., 8(48), 27429-27437. https://doi.org/10.1039/c8ra04178d
Mančić L, Đukić-Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot A, Costa AMLM, Marinković BA, Mojović L, Milošević O. One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. in RSC Advances. 2018;8(48):27429-27437. doi:10.1039/c8ra04178d .
Mančić, Lidija, Đukić-Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar, Costa, Antonio Mario Leal Martins, Marinković, Bojan A., Mojović, Ljiljana, Milošević, Olivera, "One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging" in RSC Advances, 8, no. 48 (2018):27429-27437, https://doi.org/10.1039/c8ra04178d . .