Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization
Само за регистроване кориснике
2018
Аутори
Carević, MilicaVukašinović-Sekulić, Maja
Ćorović, Marija
Rogniaux, Helene
Ropartz, David
Veličković, Dušan
Bezbradica, Dejan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
beta-Galactosidase is an important industrial enzyme that catalyzes reaction of lactose hydrolysis and recently more interesting reaction of transgalactosylation, yielding a highly valuable group of prebiotic compounds named galacto-oligosaccharides (GOS). In this paper, parameters for achieving high yields of tailor-made GOS using crude beta-galactosidase obtained from Lactobacillus acidophilus ATCC 4356, probiotic bacteria regarded as safe for human consumption, were optimized. At the same time, detailed structural elucidation of obtained GOS was conducted, and it was concluded that beta-galactosidase from L acidophilus shows a particular specificity towards the formation of beta-(1 - gt 6) glycosidic bonds. In order to develop more stable and economically cost-effective preparation, crude enzyme was successfully immobilized on a methacrylic polymer carrier Lifetech ECR8409, leading to its simultaneous 2-fold purification. This immobilized preparation showed unchanged specificity to...wards the transgalactosylation reaction, thus yielding 86 WI GOS under the previously optimized conditions (lactose concentration 400 g/l in 0.1 M sodium phosphate buffer, pH 6.8 and temperature 50 degrees C).
Кључне речи:
beta-Galactosidase / Immobilization / Lactobacillus acidophilus / Galacto-oligosaccharides / Lifetech ECR resinsИзвор:
Journal of Bioscience and Bioengineering, 2018, 126, 6, 697-704Издавач:
- Soc Bioscience Bioengineering Japan, Osaka
Финансирање / пројекти:
DOI: 10.1016/j.jbiosc.2018.06.003
ISSN: 1389-1723
PubMed: 30401452
WoS: 000452933700005
Scopus: 2-s2.0-85049305185
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Carević, Milica AU - Vukašinović-Sekulić, Maja AU - Ćorović, Marija AU - Rogniaux, Helene AU - Ropartz, David AU - Veličković, Dušan AU - Bezbradica, Dejan PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3934 AB - beta-Galactosidase is an important industrial enzyme that catalyzes reaction of lactose hydrolysis and recently more interesting reaction of transgalactosylation, yielding a highly valuable group of prebiotic compounds named galacto-oligosaccharides (GOS). In this paper, parameters for achieving high yields of tailor-made GOS using crude beta-galactosidase obtained from Lactobacillus acidophilus ATCC 4356, probiotic bacteria regarded as safe for human consumption, were optimized. At the same time, detailed structural elucidation of obtained GOS was conducted, and it was concluded that beta-galactosidase from L acidophilus shows a particular specificity towards the formation of beta-(1 - gt 6) glycosidic bonds. In order to develop more stable and economically cost-effective preparation, crude enzyme was successfully immobilized on a methacrylic polymer carrier Lifetech ECR8409, leading to its simultaneous 2-fold purification. This immobilized preparation showed unchanged specificity towards the transgalactosylation reaction, thus yielding 86 WI GOS under the previously optimized conditions (lactose concentration 400 g/l in 0.1 M sodium phosphate buffer, pH 6.8 and temperature 50 degrees C). PB - Soc Bioscience Bioengineering Japan, Osaka T2 - Journal of Bioscience and Bioengineering T1 - Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization EP - 704 IS - 6 SP - 697 VL - 126 DO - 10.1016/j.jbiosc.2018.06.003 ER -
@article{ author = "Carević, Milica and Vukašinović-Sekulić, Maja and Ćorović, Marija and Rogniaux, Helene and Ropartz, David and Veličković, Dušan and Bezbradica, Dejan", year = "2018", abstract = "beta-Galactosidase is an important industrial enzyme that catalyzes reaction of lactose hydrolysis and recently more interesting reaction of transgalactosylation, yielding a highly valuable group of prebiotic compounds named galacto-oligosaccharides (GOS). In this paper, parameters for achieving high yields of tailor-made GOS using crude beta-galactosidase obtained from Lactobacillus acidophilus ATCC 4356, probiotic bacteria regarded as safe for human consumption, were optimized. At the same time, detailed structural elucidation of obtained GOS was conducted, and it was concluded that beta-galactosidase from L acidophilus shows a particular specificity towards the formation of beta-(1 - gt 6) glycosidic bonds. In order to develop more stable and economically cost-effective preparation, crude enzyme was successfully immobilized on a methacrylic polymer carrier Lifetech ECR8409, leading to its simultaneous 2-fold purification. This immobilized preparation showed unchanged specificity towards the transgalactosylation reaction, thus yielding 86 WI GOS under the previously optimized conditions (lactose concentration 400 g/l in 0.1 M sodium phosphate buffer, pH 6.8 and temperature 50 degrees C).", publisher = "Soc Bioscience Bioengineering Japan, Osaka", journal = "Journal of Bioscience and Bioengineering", title = "Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization", pages = "704-697", number = "6", volume = "126", doi = "10.1016/j.jbiosc.2018.06.003" }
Carević, M., Vukašinović-Sekulić, M., Ćorović, M., Rogniaux, H., Ropartz, D., Veličković, D.,& Bezbradica, D.. (2018). Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization. in Journal of Bioscience and Bioengineering Soc Bioscience Bioengineering Japan, Osaka., 126(6), 697-704. https://doi.org/10.1016/j.jbiosc.2018.06.003
Carević M, Vukašinović-Sekulić M, Ćorović M, Rogniaux H, Ropartz D, Veličković D, Bezbradica D. Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization. in Journal of Bioscience and Bioengineering. 2018;126(6):697-704. doi:10.1016/j.jbiosc.2018.06.003 .
Carević, Milica, Vukašinović-Sekulić, Maja, Ćorović, Marija, Rogniaux, Helene, Ropartz, David, Veličković, Dušan, Bezbradica, Dejan, "Evaluation of beta-galactosidase from Lactobacillus acidophilus as biocatalyst for galacto-oligosaccharides synthesis: Product structural characterization and enzyme immobilization" in Journal of Bioscience and Bioengineering, 126, no. 6 (2018):697-704, https://doi.org/10.1016/j.jbiosc.2018.06.003 . .