Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods
Само за регистроване кориснике
2019
Аутори
Salim, Abdalla AliGrbavčić, Sanja
Šekuljica, Nataša
Vukašinović-Sekulić, Maja
Jovanović, Jelena
Jakovetić Tanasković, Sonja
Luković, Nevena
Knežević-Jugović, Zorica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Protease, cellulase, and alpha-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and alpha-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity ( gt = 7.5 IU).
Кључне речи:
Bacillus subtilis / protease / cellulase / alpha-amylase / solid-state fermentation / ultrasound / extractionИзвор:
Biotechnology and Applied Biochemistry, 2019, 66, 3, 361-368Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
DOI: 10.1002/bab.1732
ISSN: 0885-4513
PubMed: 30694578
WoS: 000476792600011
Scopus: 2-s2.0-85061254940
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Salim, Abdalla Ali AU - Grbavčić, Sanja AU - Šekuljica, Nataša AU - Vukašinović-Sekulić, Maja AU - Jovanović, Jelena AU - Jakovetić Tanasković, Sonja AU - Luković, Nevena AU - Knežević-Jugović, Zorica PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4065 AB - Protease, cellulase, and alpha-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and alpha-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity ( gt = 7.5 IU). PB - Wiley, Hoboken T2 - Biotechnology and Applied Biochemistry T1 - Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods EP - 368 IS - 3 SP - 361 VL - 66 DO - 10.1002/bab.1732 ER -
@article{ author = "Salim, Abdalla Ali and Grbavčić, Sanja and Šekuljica, Nataša and Vukašinović-Sekulić, Maja and Jovanović, Jelena and Jakovetić Tanasković, Sonja and Luković, Nevena and Knežević-Jugović, Zorica", year = "2019", abstract = "Protease, cellulase, and alpha-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and alpha-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity ( gt = 7.5 IU).", publisher = "Wiley, Hoboken", journal = "Biotechnology and Applied Biochemistry", title = "Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods", pages = "368-361", number = "3", volume = "66", doi = "10.1002/bab.1732" }
Salim, A. A., Grbavčić, S., Šekuljica, N., Vukašinović-Sekulić, M., Jovanović, J., Jakovetić Tanasković, S., Luković, N.,& Knežević-Jugović, Z.. (2019). Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods. in Biotechnology and Applied Biochemistry Wiley, Hoboken., 66(3), 361-368. https://doi.org/10.1002/bab.1732
Salim AA, Grbavčić S, Šekuljica N, Vukašinović-Sekulić M, Jovanović J, Jakovetić Tanasković S, Luković N, Knežević-Jugović Z. Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods. in Biotechnology and Applied Biochemistry. 2019;66(3):361-368. doi:10.1002/bab.1732 .
Salim, Abdalla Ali, Grbavčić, Sanja, Šekuljica, Nataša, Vukašinović-Sekulić, Maja, Jovanović, Jelena, Jakovetić Tanasković, Sonja, Luković, Nevena, Knežević-Jugović, Zorica, "Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods" in Biotechnology and Applied Biochemistry, 66, no. 3 (2019):361-368, https://doi.org/10.1002/bab.1732 . .