Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy
Само за регистроване кориснике
2019
Аутори
Lazarević, J. J.Ralević, U.
Kukolj, Tamara
Bugarski, Diana
Lazarević, N.
Bugarski, Branko
Popović, Z., V
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different... chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.
Кључне речи:
Raman spectroscopy / Stem cells / Chemical fixationИзвор:
Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 2019, 216, 173-178Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Наноструктурни мултифункционални материјали и нанокомпозити (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45018)
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-MESTD-Basic Research (BR or ON)-175062)
- Физика уређених наноструктура и нових материјала у фотоници (RS-MESTD-Basic Research (BR or ON)-171005)
DOI: 10.1016/j.saa.2019.03.012
ISSN: 1386-1425
PubMed: 30897378
WoS: 000471317300021
Scopus: 2-s2.0-85062988764
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Lazarević, J. J. AU - Ralević, U. AU - Kukolj, Tamara AU - Bugarski, Diana AU - Lazarević, N. AU - Bugarski, Branko AU - Popović, Z., V PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4213 AB - In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy T1 - Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy EP - 178 SP - 173 VL - 216 DO - 10.1016/j.saa.2019.03.012 ER -
@article{ author = "Lazarević, J. J. and Ralević, U. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V", year = "2019", abstract = "In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy", title = "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy", pages = "178-173", volume = "216", doi = "10.1016/j.saa.2019.03.012" }
Lazarević, J. J., Ralević, U., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy Pergamon-Elsevier Science Ltd, Oxford., 216, 173-178. https://doi.org/10.1016/j.saa.2019.03.012
Lazarević JJ, Ralević U, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2019;216:173-178. doi:10.1016/j.saa.2019.03.012 .
Lazarević, J. J., Ralević, U., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 216 (2019):173-178, https://doi.org/10.1016/j.saa.2019.03.012 . .