Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential
Authorized Users Only
2020
Authors
Šekuljica, NatašaJovanović, Jelena
Jakovetić Tanasković, Sonja
Ognjanović, Nevena
Gazikalović, Ivana
Knežević-Jugović, Zorica
Mijin, Dušan
Article (Published version)
Metadata
Show full item recordAbstract
Horseradish peroxidase (HRP) is a highly specific enzyme with great potential for use in the decolorization of synthetic dyes. A comprehensive study of HRP immobilization using various techniques such as adsorption and covalent immobilization on the novel carrier Purolite (R) A109 with a special focus on enzymatic decolorization and toxicity of artificially colored wastewater. The immobilized preparations with an activity of 156.21 +/- 1.41 U g(-1) and 85.71 +/- 1.62 U g(-1) after the HRP adsorption and covalent immobilization, respectively, were obtained. Stability and reusability of the immobilized preparations were also evaluated. A noteworthy decolorization level (similar to 90%) with immobilized HRP was achieved. Phytotoxicity testing using Mung bean seeds and acute toxicity assay with Artemia salina has confirmed the applicability of the obtained immobilized preparation in industrial wastewater plants for the treatment of colored wastewater.
Keywords:
biodegradation / horseradish peroxidase / immobilization / synthetic dyes / toxicity / wastewaterSource:
Biotechnology Progress, 2020, 36, 4Publisher:
- Wiley, Hoboken
Funding / projects:
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-172013)
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
DOI: 10.1002/btpr.2991
ISSN: 8756-7938
PubMed: 32170846
WoS: 000521621100001
Scopus: 2-s2.0-85082310685
Collections
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Šekuljica, Nataša AU - Jovanović, Jelena AU - Jakovetić Tanasković, Sonja AU - Ognjanović, Nevena AU - Gazikalović, Ivana AU - Knežević-Jugović, Zorica AU - Mijin, Dušan PY - 2020 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4457 AB - Horseradish peroxidase (HRP) is a highly specific enzyme with great potential for use in the decolorization of synthetic dyes. A comprehensive study of HRP immobilization using various techniques such as adsorption and covalent immobilization on the novel carrier Purolite (R) A109 with a special focus on enzymatic decolorization and toxicity of artificially colored wastewater. The immobilized preparations with an activity of 156.21 +/- 1.41 U g(-1) and 85.71 +/- 1.62 U g(-1) after the HRP adsorption and covalent immobilization, respectively, were obtained. Stability and reusability of the immobilized preparations were also evaluated. A noteworthy decolorization level (similar to 90%) with immobilized HRP was achieved. Phytotoxicity testing using Mung bean seeds and acute toxicity assay with Artemia salina has confirmed the applicability of the obtained immobilized preparation in industrial wastewater plants for the treatment of colored wastewater. PB - Wiley, Hoboken T2 - Biotechnology Progress T1 - Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential IS - 4 VL - 36 DO - 10.1002/btpr.2991 ER -
@article{ author = "Šekuljica, Nataša and Jovanović, Jelena and Jakovetić Tanasković, Sonja and Ognjanović, Nevena and Gazikalović, Ivana and Knežević-Jugović, Zorica and Mijin, Dušan", year = "2020", abstract = "Horseradish peroxidase (HRP) is a highly specific enzyme with great potential for use in the decolorization of synthetic dyes. A comprehensive study of HRP immobilization using various techniques such as adsorption and covalent immobilization on the novel carrier Purolite (R) A109 with a special focus on enzymatic decolorization and toxicity of artificially colored wastewater. The immobilized preparations with an activity of 156.21 +/- 1.41 U g(-1) and 85.71 +/- 1.62 U g(-1) after the HRP adsorption and covalent immobilization, respectively, were obtained. Stability and reusability of the immobilized preparations were also evaluated. A noteworthy decolorization level (similar to 90%) with immobilized HRP was achieved. Phytotoxicity testing using Mung bean seeds and acute toxicity assay with Artemia salina has confirmed the applicability of the obtained immobilized preparation in industrial wastewater plants for the treatment of colored wastewater.", publisher = "Wiley, Hoboken", journal = "Biotechnology Progress", title = "Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential", number = "4", volume = "36", doi = "10.1002/btpr.2991" }
Šekuljica, N., Jovanović, J., Jakovetić Tanasković, S., Ognjanović, N., Gazikalović, I., Knežević-Jugović, Z.,& Mijin, D.. (2020). Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential. in Biotechnology Progress Wiley, Hoboken., 36(4). https://doi.org/10.1002/btpr.2991
Šekuljica N, Jovanović J, Jakovetić Tanasković S, Ognjanović N, Gazikalović I, Knežević-Jugović Z, Mijin D. Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential. in Biotechnology Progress. 2020;36(4). doi:10.1002/btpr.2991 .
Šekuljica, Nataša, Jovanović, Jelena, Jakovetić Tanasković, Sonja, Ognjanović, Nevena, Gazikalović, Ivana, Knežević-Jugović, Zorica, Mijin, Dušan, "Immobilization of horseradish peroxidase onto Purolite (R) A109 and its anthraquinone dye biodegradation and detoxification potential" in Biotechnology Progress, 36, no. 4 (2020), https://doi.org/10.1002/btpr.2991 . .