dc.creator | Velicić, Zorica | |
dc.creator | Rusmirović, Jelena | |
dc.creator | Prlainović, Nevena | |
dc.creator | Tomić, Nataša | |
dc.creator | Veličković, Zlate | |
dc.creator | Taleb, Khaled | |
dc.creator | Marinković, Aleksandar | |
dc.date.accessioned | 2021-03-10T14:20:22Z | |
dc.date.available | 2021-03-10T14:20:22Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1022-9760 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4461 | |
dc.description.abstract | In this work, the optimization of synthesis of terpolymer monolith based on glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA) and additional cross-linkers: trimethylolpropanetriacrylate (TMPTA) or triethylene glycol dimethacrylate (TEGDMA) was performed. Moreover, novel vinyl functionalized cross-linkable polymers: ethanolamine (EA)/methacryloyl (MAC) modified poly (methyl methacrylate) (PMMA), and hydrolyzed poly (ethylene-co-vinyl acetate) copolymer (EVOH) modified with MAC either directly or via ethyl malonyl chloride/EA bridging group (m-EVA) were used as cross-linkable polymer to improve mechanical/elastic properties of the obtained monoliths. Optimization procedure, performed applying response surface methodology (RSM), was focused on the production of materials with improved dimensional stability/integrity and porosity with abundance of epoxide groups capable for immobilization of lipase from Candida rugosa (CRL). Structural characterization of the synthesized monoliths was determined using FTIR, Raman and H-1 NMR spectroscopies, while morphology/porosity was determined by SEM technique and image analysis; and mechanical properties by diametral compression testing. The most potential monolith containing m-EVA polymeric cross-linker, i.e. GMA/EGDMA/TEGDMA/m-EVA monolith, was used as CRL carrier in a two-step immobilization process. Enzyme loading and the activity of obtained preparations for various initial enzyme concentrations were monitored after 4 and 48 h of immobilization. The resulting catalysts show high potency in biocatalytic reactions with the highest percentage of retained initial lipase activity of 64.5%. | en |
dc.publisher | Springer, Dordrecht | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200325/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of Polymer Research | |
dc.subject | Macroporous copolymers | en |
dc.subject | response surface methodology (RSM) | en |
dc.subject | Candida rugosa lipase | en |
dc.subject | Enzyme immobilization | en |
dc.subject | Compression testing | en |
dc.title | The optimization of glycidyl methacrylate based terpolymer monolith synthesis: an effective Candida rugosa lipase immobilization support | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.issue | 5 | |
dc.citation.other | 27(5): - | |
dc.citation.rank | M22 | |
dc.citation.volume | 27 | |
dc.identifier.doi | 10.1007/s10965-020-02127-z | |
dc.identifier.scopus | 2-s2.0-85084144196 | |
dc.identifier.wos | 000531408400004 | |
dc.type.version | publishedVersion | |