The chitosan-based bioactive composite coating on titanium
Authors
Stevanović, MilenaĐošić, Marija
Janković, Ana
Kojić, Vesna
Stojanović, Jovica
Grujić, Svetlana
Matić-Bujagić, Ivana
Rhee, Kyong Yop
Mišković-Stanković, Vesna
Article (Published version)
Metadata
Show full item recordAbstract
This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapi...d release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.
Keywords:
Coating / Hydroxyapatite / Chitosan / Graphene / Bioactivity / GentamicinSource:
Journal of Materials Research and Technology, 2021, 15, 4461-4474Publisher:
- Elsevier B.V.
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
DOI: 10.1016/j.jmrt.2021.10.072
ISSN: 2238-7854
WoS: 000717684300003
Scopus: 2-s2.0-85118276778
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Stevanović, Milena AU - Đošić, Marija AU - Janković, Ana AU - Kojić, Vesna AU - Stojanović, Jovica AU - Grujić, Svetlana AU - Matić-Bujagić, Ivana AU - Rhee, Kyong Yop AU - Mišković-Stanković, Vesna PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4839 AB - This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days. PB - Elsevier B.V. T2 - Journal of Materials Research and Technology T1 - The chitosan-based bioactive composite coating on titanium EP - 4474 SP - 4461 VL - 15 DO - 10.1016/j.jmrt.2021.10.072 ER -
@article{ author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna", year = "2021", abstract = "This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.", publisher = "Elsevier B.V.", journal = "Journal of Materials Research and Technology", title = "The chitosan-based bioactive composite coating on titanium", pages = "4474-4461", volume = "15", doi = "10.1016/j.jmrt.2021.10.072" }
Stevanović, M., Đošić, M., Janković, A., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2021). The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology Elsevier B.V.., 15, 4461-4474. https://doi.org/10.1016/j.jmrt.2021.10.072
Stevanović M, Đošić M, Janković A, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology. 2021;15:4461-4474. doi:10.1016/j.jmrt.2021.10.072 .
Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "The chitosan-based bioactive composite coating on titanium" in Journal of Materials Research and Technology, 15 (2021):4461-4474, https://doi.org/10.1016/j.jmrt.2021.10.072 . .