Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?
Аутори
Vork, LisaPenders, John
Jalanka, Jonna
Bojic, Svetlana
van Kuijk, Sander M. J.
Salonen, Anne
de Vos, Willem M.
Rajilić-Stojanović, Mirjana
Weerts, Zsa Zsa R. M.
Masclee, Ad A. M.
Pozuelo, Marta
Manichanh, Chaysavanh
Jonkers, Daisy M. A. E.
Чланак у часопису (Објављена верзија)
Метаподаци
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Introduction Stool consistency has been associated with fecal microbial composition. Stool consistency often varies over time, in subjects with and without gastrointestinal disorders, raising the question whether variability in the microbial composition should be considered in microbiota studies. We evaluated within-subject day-to-day variability in stool consistency and the association with the fecal microbiota in irritable bowel syndrome (IBS) and healthy subjects, over seven days. Methods Twelve IBS patients and 12 healthy subjects collected fecal samples during seven consecutive days. Stool consistency was determined by the patient-reported Bristol Stool Scale (BSS) and fecal dry weight percentage. 16S rRNA V4 gene sequencing was performed and microbial richness (alpha diversity; Chao1 index, observed number of species, effective Shannon index) and microbial community structure (beta diversity; Bray-Curtis distance, generalized UniFrac, and taxa abundance on family level) were dete...rmined. Results Linear mixed-effects models showed significant associations between stool consistency and microbial richness, but no time effect. This implies that between-subject but not within-subject variation in microbiota over time can partially be explained by variation in stool consistency. Redundancy analysis showed a significant association between stool consistency and microbial community structure, but additional linear mixed-effects models did not demonstrate a time effect on this. Conclusion This study supports an association between stool consistency and fecal microbiota, but no effect of day-to-day fluctuations in stool consistency within seven days. This consolidates the importance of considering stool consistency in gut microbiota research, though confirms the validity of single fecal sampling to represent an individual's microbiota at a given time point. NCT00775060.
Кључне речи:
fecal microbiota / intestinal microbiome / stool consistency / irritable bowel syndrome / adultИзвор:
Frontiers in Cellular and Infection Microbiology, 2021, 11
DOI: 10.3389/fcimb.2021.639667
ISSN: 2235-2988
PubMed: 34458156
WoS: 000680709300001
Scopus: 2-s2.0-85111934785
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Vork, Lisa AU - Penders, John AU - Jalanka, Jonna AU - Bojic, Svetlana AU - van Kuijk, Sander M. J. AU - Salonen, Anne AU - de Vos, Willem M. AU - Rajilić-Stojanović, Mirjana AU - Weerts, Zsa Zsa R. M. AU - Masclee, Ad A. M. AU - Pozuelo, Marta AU - Manichanh, Chaysavanh AU - Jonkers, Daisy M. A. E. PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4854 AB - Introduction Stool consistency has been associated with fecal microbial composition. Stool consistency often varies over time, in subjects with and without gastrointestinal disorders, raising the question whether variability in the microbial composition should be considered in microbiota studies. We evaluated within-subject day-to-day variability in stool consistency and the association with the fecal microbiota in irritable bowel syndrome (IBS) and healthy subjects, over seven days. Methods Twelve IBS patients and 12 healthy subjects collected fecal samples during seven consecutive days. Stool consistency was determined by the patient-reported Bristol Stool Scale (BSS) and fecal dry weight percentage. 16S rRNA V4 gene sequencing was performed and microbial richness (alpha diversity; Chao1 index, observed number of species, effective Shannon index) and microbial community structure (beta diversity; Bray-Curtis distance, generalized UniFrac, and taxa abundance on family level) were determined. Results Linear mixed-effects models showed significant associations between stool consistency and microbial richness, but no time effect. This implies that between-subject but not within-subject variation in microbiota over time can partially be explained by variation in stool consistency. Redundancy analysis showed a significant association between stool consistency and microbial community structure, but additional linear mixed-effects models did not demonstrate a time effect on this. Conclusion This study supports an association between stool consistency and fecal microbiota, but no effect of day-to-day fluctuations in stool consistency within seven days. This consolidates the importance of considering stool consistency in gut microbiota research, though confirms the validity of single fecal sampling to represent an individual's microbiota at a given time point. NCT00775060. T2 - Frontiers in Cellular and Infection Microbiology T1 - Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition? VL - 11 DO - 10.3389/fcimb.2021.639667 ER -
@article{ author = "Vork, Lisa and Penders, John and Jalanka, Jonna and Bojic, Svetlana and van Kuijk, Sander M. J. and Salonen, Anne and de Vos, Willem M. and Rajilić-Stojanović, Mirjana and Weerts, Zsa Zsa R. M. and Masclee, Ad A. M. and Pozuelo, Marta and Manichanh, Chaysavanh and Jonkers, Daisy M. A. E.", year = "2021", abstract = "Introduction Stool consistency has been associated with fecal microbial composition. Stool consistency often varies over time, in subjects with and without gastrointestinal disorders, raising the question whether variability in the microbial composition should be considered in microbiota studies. We evaluated within-subject day-to-day variability in stool consistency and the association with the fecal microbiota in irritable bowel syndrome (IBS) and healthy subjects, over seven days. Methods Twelve IBS patients and 12 healthy subjects collected fecal samples during seven consecutive days. Stool consistency was determined by the patient-reported Bristol Stool Scale (BSS) and fecal dry weight percentage. 16S rRNA V4 gene sequencing was performed and microbial richness (alpha diversity; Chao1 index, observed number of species, effective Shannon index) and microbial community structure (beta diversity; Bray-Curtis distance, generalized UniFrac, and taxa abundance on family level) were determined. Results Linear mixed-effects models showed significant associations between stool consistency and microbial richness, but no time effect. This implies that between-subject but not within-subject variation in microbiota over time can partially be explained by variation in stool consistency. Redundancy analysis showed a significant association between stool consistency and microbial community structure, but additional linear mixed-effects models did not demonstrate a time effect on this. Conclusion This study supports an association between stool consistency and fecal microbiota, but no effect of day-to-day fluctuations in stool consistency within seven days. This consolidates the importance of considering stool consistency in gut microbiota research, though confirms the validity of single fecal sampling to represent an individual's microbiota at a given time point. NCT00775060.", journal = "Frontiers in Cellular and Infection Microbiology", title = "Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?", volume = "11", doi = "10.3389/fcimb.2021.639667" }
Vork, L., Penders, J., Jalanka, J., Bojic, S., van Kuijk, S. M. J., Salonen, A., de Vos, W. M., Rajilić-Stojanović, M., Weerts, Z. Z. R. M., Masclee, A. A. M., Pozuelo, M., Manichanh, C.,& Jonkers, D. M. A. E.. (2021). Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?. in Frontiers in Cellular and Infection Microbiology, 11. https://doi.org/10.3389/fcimb.2021.639667
Vork L, Penders J, Jalanka J, Bojic S, van Kuijk SMJ, Salonen A, de Vos WM, Rajilić-Stojanović M, Weerts ZZRM, Masclee AAM, Pozuelo M, Manichanh C, Jonkers DMAE. Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?. in Frontiers in Cellular and Infection Microbiology. 2021;11. doi:10.3389/fcimb.2021.639667 .
Vork, Lisa, Penders, John, Jalanka, Jonna, Bojic, Svetlana, van Kuijk, Sander M. J., Salonen, Anne, de Vos, Willem M., Rajilić-Stojanović, Mirjana, Weerts, Zsa Zsa R. M., Masclee, Ad A. M., Pozuelo, Marta, Manichanh, Chaysavanh, Jonkers, Daisy M. A. E., "Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?" in Frontiers in Cellular and Infection Microbiology, 11 (2021), https://doi.org/10.3389/fcimb.2021.639667 . .