Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate
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2021
Authors
Katić, KatarinaBanjanac, Katarina
Simović, Milica
Ćorović, Marija
Milivojević, Ana
Marinković, Aleksandar
Bezbradica, Dejan
Article (Published version)
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In this study, the suitability of fumed silica nanoparticles (FNS) and its derivatives (amino-modified FNS (AFNS), cyanuric chloride-activated AFNS (CCAFNS) and epoxy-modified FNS (GFNS)), for covalent immobilisation of two commercial protease preparations Alcalase(R) and Flavourzyme(R) was investigated. The highest hydrolytic activities of immobilised preparations were 25 IU g(-1) support (Alcalase-GFNS) and 2.95 IU g(-1) support (Flavourzyme-CCAFNS). Furthermore, the immobilised preparations showed 43% and 20% of initial specific activities of commercial protease preparations, respectively. Flavourzyme-CCAFNS also exhibited the highest exopeptidase activity of 22.83 L-pNAU g(-1) support. Finally, these two nanobiocatalysts were successfully applied for hydrolysis of sunflower meal protein isolate (SMPI), providing two times higher hydrolysis yields in comparison to free enzymes, justifying the applied immobilisation process. Namely, the highest hydrolysis yield (30%) was gained by th...e sequential hydrolysis with Alcalase-GFNS and Flavourzyme-CCAFNS, which resulted in the formation of small hydrophobic and hydrophilic peptides, lt = 5 kDa, confirmed by HPLC analysis and electrophoretic separation.
Keywords:
Alcalase / covalent immobilisation / Flavourzyme / nanobiocatalyst / protein hydrolysates / sunflower mealSource:
International Journal of Food Science and Technology, 2021, 56, 9, 4287-4297Publisher:
- John Wiley and Sons Inc.
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
DOI: 10.1111/ijfs.15189
ISSN: 0950-5423
WoS: 000672015400001
Scopus: 2-s2.0-85109742602
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Inovacioni centarTY - JOUR AU - Katić, Katarina AU - Banjanac, Katarina AU - Simović, Milica AU - Ćorović, Marija AU - Milivojević, Ana AU - Marinković, Aleksandar AU - Bezbradica, Dejan PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4864 AB - In this study, the suitability of fumed silica nanoparticles (FNS) and its derivatives (amino-modified FNS (AFNS), cyanuric chloride-activated AFNS (CCAFNS) and epoxy-modified FNS (GFNS)), for covalent immobilisation of two commercial protease preparations Alcalase(R) and Flavourzyme(R) was investigated. The highest hydrolytic activities of immobilised preparations were 25 IU g(-1) support (Alcalase-GFNS) and 2.95 IU g(-1) support (Flavourzyme-CCAFNS). Furthermore, the immobilised preparations showed 43% and 20% of initial specific activities of commercial protease preparations, respectively. Flavourzyme-CCAFNS also exhibited the highest exopeptidase activity of 22.83 L-pNAU g(-1) support. Finally, these two nanobiocatalysts were successfully applied for hydrolysis of sunflower meal protein isolate (SMPI), providing two times higher hydrolysis yields in comparison to free enzymes, justifying the applied immobilisation process. Namely, the highest hydrolysis yield (30%) was gained by the sequential hydrolysis with Alcalase-GFNS and Flavourzyme-CCAFNS, which resulted in the formation of small hydrophobic and hydrophilic peptides, lt = 5 kDa, confirmed by HPLC analysis and electrophoretic separation. PB - John Wiley and Sons Inc. T2 - International Journal of Food Science and Technology T1 - Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate EP - 4297 IS - 9 SP - 4287 VL - 56 DO - 10.1111/ijfs.15189 ER -
@article{ author = "Katić, Katarina and Banjanac, Katarina and Simović, Milica and Ćorović, Marija and Milivojević, Ana and Marinković, Aleksandar and Bezbradica, Dejan", year = "2021", abstract = "In this study, the suitability of fumed silica nanoparticles (FNS) and its derivatives (amino-modified FNS (AFNS), cyanuric chloride-activated AFNS (CCAFNS) and epoxy-modified FNS (GFNS)), for covalent immobilisation of two commercial protease preparations Alcalase(R) and Flavourzyme(R) was investigated. The highest hydrolytic activities of immobilised preparations were 25 IU g(-1) support (Alcalase-GFNS) and 2.95 IU g(-1) support (Flavourzyme-CCAFNS). Furthermore, the immobilised preparations showed 43% and 20% of initial specific activities of commercial protease preparations, respectively. Flavourzyme-CCAFNS also exhibited the highest exopeptidase activity of 22.83 L-pNAU g(-1) support. Finally, these two nanobiocatalysts were successfully applied for hydrolysis of sunflower meal protein isolate (SMPI), providing two times higher hydrolysis yields in comparison to free enzymes, justifying the applied immobilisation process. Namely, the highest hydrolysis yield (30%) was gained by the sequential hydrolysis with Alcalase-GFNS and Flavourzyme-CCAFNS, which resulted in the formation of small hydrophobic and hydrophilic peptides, lt = 5 kDa, confirmed by HPLC analysis and electrophoretic separation.", publisher = "John Wiley and Sons Inc.", journal = "International Journal of Food Science and Technology", title = "Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate", pages = "4297-4287", number = "9", volume = "56", doi = "10.1111/ijfs.15189" }
Katić, K., Banjanac, K., Simović, M., Ćorović, M., Milivojević, A., Marinković, A.,& Bezbradica, D.. (2021). Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate. in International Journal of Food Science and Technology John Wiley and Sons Inc.., 56(9), 4287-4297. https://doi.org/10.1111/ijfs.15189
Katić K, Banjanac K, Simović M, Ćorović M, Milivojević A, Marinković A, Bezbradica D. Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate. in International Journal of Food Science and Technology. 2021;56(9):4287-4297. doi:10.1111/ijfs.15189 .
Katić, Katarina, Banjanac, Katarina, Simović, Milica, Ćorović, Marija, Milivojević, Ana, Marinković, Aleksandar, Bezbradica, Dejan, "Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate" in International Journal of Food Science and Technology, 56, no. 9 (2021):4287-4297, https://doi.org/10.1111/ijfs.15189 . .