Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions
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2021
Authors
Stojkovska, JasminaZvicer, Jovana
Andrejevic, Milica
Janaćković, Đorđe
Obradović, Bojana
Veljović, Đorđe
Article (Published version)
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In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure. HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and beta-tricalcium phosphate, respectively. Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy. Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased. HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution w...ith [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor. The continuous SBF flow (superficial velocity of 400 mu m/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds. These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.
Keywords:
biomimetic perfusion bioreactor / bone tissue engineering / macroporous scaffolds / tricalcium phosphateSource:
Journal of Biomedical Materials Research Part B-Applied Biomaterials, 2021, 109, 12, 2079-2090Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
DOI: 10.1002/jbm.b.34856
ISSN: 1552-4973
PubMed: 33955159
WoS: 000647263300001
Scopus: 2-s2.0-85105109481
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Tehnološko-metalurški fakultetTY - JOUR AU - Stojkovska, Jasmina AU - Zvicer, Jovana AU - Andrejevic, Milica AU - Janaćković, Đorđe AU - Obradović, Bojana AU - Veljović, Đorđe PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4934 AB - In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure. HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and beta-tricalcium phosphate, respectively. Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy. Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased. HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor. The continuous SBF flow (superficial velocity of 400 mu m/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds. These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering. T2 - Journal of Biomedical Materials Research Part B-Applied Biomaterials T1 - Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions EP - 2090 IS - 12 SP - 2079 VL - 109 DO - 10.1002/jbm.b.34856 ER -
@article{ author = "Stojkovska, Jasmina and Zvicer, Jovana and Andrejevic, Milica and Janaćković, Đorđe and Obradović, Bojana and Veljović, Đorđe", year = "2021", abstract = "In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure. HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and beta-tricalcium phosphate, respectively. Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy. Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased. HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor. The continuous SBF flow (superficial velocity of 400 mu m/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds. These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.", journal = "Journal of Biomedical Materials Research Part B-Applied Biomaterials", title = "Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions", pages = "2090-2079", number = "12", volume = "109", doi = "10.1002/jbm.b.34856" }
Stojkovska, J., Zvicer, J., Andrejevic, M., Janaćković, Đ., Obradović, B.,& Veljović, Đ.. (2021). Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions. in Journal of Biomedical Materials Research Part B-Applied Biomaterials, 109(12), 2079-2090. https://doi.org/10.1002/jbm.b.34856
Stojkovska J, Zvicer J, Andrejevic M, Janaćković Đ, Obradović B, Veljović Đ. Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions. in Journal of Biomedical Materials Research Part B-Applied Biomaterials. 2021;109(12):2079-2090. doi:10.1002/jbm.b.34856 .
Stojkovska, Jasmina, Zvicer, Jovana, Andrejevic, Milica, Janaćković, Đorđe, Obradović, Bojana, Veljović, Đorđe, "Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions" in Journal of Biomedical Materials Research Part B-Applied Biomaterials, 109, no. 12 (2021):2079-2090, https://doi.org/10.1002/jbm.b.34856 . .