Biodegradation of poly(ε-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil
Аутори
Mandić, MinaSpasić, Jelena
Ponjavić, Marijana
Nikolić, Marija
Ćosović, Vladan
O'Connor, Kevin E
Nikodinović-Runić, Jasmina
Đokić, Lidija
Jeremić, Sanja
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly(ε-caprolactone) (PCL) or polyhydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 μm) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 μm) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chlororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, P...CL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis B-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers.
Кључне речи:
Biodegradation / Biopolymers / Compost / Enzymes / Pseudomonas / StreptomycesИзвор:
Polymer Degradation and Stability, 2019, 162, 160-168Издавач:
- Elsevier Ltd
Финансирање / пројекти:
- Синтеза и карактеризација нових функционалних полимера и полимерних нанокомпозита (RS-MESTD-Basic Research (BR or ON)-172062)
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
Напомена:
- Published version: http://technorep.tmf.bg.ac.rs/handle/123456789/4250
- This is the peer-reviewed version of the following article: Mandić M, Spasić J, Ponjavić M, Nikolić M, Ćosović V, O'Connor KE, Nikodinović-Runić J, Đokić L, Jeremić S. Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil. in Polymer Degradation and Stability. 2019;162:160-168. doi:10.1016/j.polymdegradstab.2019.02.012
Повезане информације:
- Верзија документа
http://technorep.tmf.bg.ac.rs/handle/123456789/4250 - Верзија документа
https://doi.org/10.1016/j.polymdegradstab.2019.02.012
DOI: 10.1016/j.polymdegradstab.2019.02.012
ISSN: 0141-3910
WoS: 000465055500019
Scopus: 2-s2.0-85062047760
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Mandić, Mina AU - Spasić, Jelena AU - Ponjavić, Marijana AU - Nikolić, Marija AU - Ćosović, Vladan AU - O'Connor, Kevin E AU - Nikodinović-Runić, Jasmina AU - Đokić, Lidija AU - Jeremić, Sanja PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5040 AB - Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly(ε-caprolactone) (PCL) or polyhydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 μm) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 μm) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chlororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, PCL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis B-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers. PB - Elsevier Ltd T2 - Polymer Degradation and Stability T1 - Biodegradation of poly(ε-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil EP - 168 SP - 160 VL - 162 DO - 10.1016/j.polymdegradstab.2019.02.012 ER -
@article{ author = "Mandić, Mina and Spasić, Jelena and Ponjavić, Marijana and Nikolić, Marija and Ćosović, Vladan and O'Connor, Kevin E and Nikodinović-Runić, Jasmina and Đokić, Lidija and Jeremić, Sanja", year = "2019", abstract = "Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly(ε-caprolactone) (PCL) or polyhydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 μm) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 μm) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chlororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, PCL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis B-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers.", publisher = "Elsevier Ltd", journal = "Polymer Degradation and Stability", title = "Biodegradation of poly(ε-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil", pages = "168-160", volume = "162", doi = "10.1016/j.polymdegradstab.2019.02.012" }
Mandić, M., Spasić, J., Ponjavić, M., Nikolić, M., Ćosović, V., O'Connor, K. E., Nikodinović-Runić, J., Đokić, L.,& Jeremić, S.. (2019). Biodegradation of poly(ε-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil. in Polymer Degradation and Stability Elsevier Ltd., 162, 160-168. https://doi.org/10.1016/j.polymdegradstab.2019.02.012
Mandić M, Spasić J, Ponjavić M, Nikolić M, Ćosović V, O'Connor KE, Nikodinović-Runić J, Đokić L, Jeremić S. Biodegradation of poly(ε-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil. in Polymer Degradation and Stability. 2019;162:160-168. doi:10.1016/j.polymdegradstab.2019.02.012 .
Mandić, Mina, Spasić, Jelena, Ponjavić, Marijana, Nikolić, Marija, Ćosović, Vladan, O'Connor, Kevin E, Nikodinović-Runić, Jasmina, Đokić, Lidija, Jeremić, Sanja, "Biodegradation of poly(ε-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil" in Polymer Degradation and Stability, 162 (2019):160-168, https://doi.org/10.1016/j.polymdegradstab.2019.02.012 . .