Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this work, functional characterization of biomaterials concerning potential application as articular cartilage implants was performed by using a biomimetic bioreactor with dynamic compression in the physiological regime (10% strain, 0.84 Hz frequency, 1 h on/1 h off). Specifically, two alginate types with low (LG) and high (HG) guluronic/mannuronic residue ratios with electrochemically synthesized silver nanoparticles (AgNPs) were evaluated. HG Ag/alginate hydrogels were clearly indicated as potential candidates due to better initial mechanical properties as compared to LG hydrogels (dynamic compression modulus of ~60 vs. ~40 kPa) as well as the mechanical stability displayed during 7 days of dynamic compression. Cytotoxicity studies in 3D bovine cartilage explant cultures under dynamic compression have shown negligible effects as compared to standard 2D monolayers of bovine chondrocytes where moderate cytotoxicity was observed. Finally, experimental and mathematical modeling studie...s revealed different mechanisms of AgNP release under physiological-like bioreactor conditions as compared to static conditions. Overall, the results clearly demonstrate bioreactor advantages in characterization and selection of candidate biomaterials as well as potentials to bridge the in vitro-in vivo gap.
Кључне речи:
3D systems / alginate types / biomimetic bioreactor / cytotoxicity / silver nanoparticlesИзвор:
Journal of Biomedical Materials Research - Part A, 2019, 107, 4, 755-768Издавач:
- John Wiley and Sons Inc.
Финансирање / пројекти:
- Синтеза, развој технологија добијања и примена наноструктурних мултифункционалних материјала дефинисаних својстава (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45019)
Напомена:
- Published version: http://technorep.tmf.bg.ac.rs/handle/123456789/4191
- This is the peer-reviewed version of the following article: Zvicer J, Mišković-Stanković V, Obradović B. Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants. in Journal of Biomedical Materials Research Part A. 2019;107(4):755-768. https://doi.org/10.1002/jbm.a.36590
Повезане информације:
- Верзија документа
http://technorep.tmf.bg.ac.rs/handle/123456789/4191 - Верзија документа
https://doi.org/10.1002/jbm.a.36590
DOI: 10.1002/jbm.a.36590
ISSN: 1549-3296
WoS: 000458824000005
Scopus: 2-s2.0-85058942586
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Zvicer, Jovana AU - Mišković-Stanković, Vesna AU - Obradović, Bojana PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5047 AB - In this work, functional characterization of biomaterials concerning potential application as articular cartilage implants was performed by using a biomimetic bioreactor with dynamic compression in the physiological regime (10% strain, 0.84 Hz frequency, 1 h on/1 h off). Specifically, two alginate types with low (LG) and high (HG) guluronic/mannuronic residue ratios with electrochemically synthesized silver nanoparticles (AgNPs) were evaluated. HG Ag/alginate hydrogels were clearly indicated as potential candidates due to better initial mechanical properties as compared to LG hydrogels (dynamic compression modulus of ~60 vs. ~40 kPa) as well as the mechanical stability displayed during 7 days of dynamic compression. Cytotoxicity studies in 3D bovine cartilage explant cultures under dynamic compression have shown negligible effects as compared to standard 2D monolayers of bovine chondrocytes where moderate cytotoxicity was observed. Finally, experimental and mathematical modeling studies revealed different mechanisms of AgNP release under physiological-like bioreactor conditions as compared to static conditions. Overall, the results clearly demonstrate bioreactor advantages in characterization and selection of candidate biomaterials as well as potentials to bridge the in vitro-in vivo gap. PB - John Wiley and Sons Inc. T2 - Journal of Biomedical Materials Research - Part A T1 - Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants EP - 768 IS - 4 SP - 755 VL - 107 DO - 10.1002/jbm.a.36590 ER -
@article{ author = "Zvicer, Jovana and Mišković-Stanković, Vesna and Obradović, Bojana", year = "2019", abstract = "In this work, functional characterization of biomaterials concerning potential application as articular cartilage implants was performed by using a biomimetic bioreactor with dynamic compression in the physiological regime (10% strain, 0.84 Hz frequency, 1 h on/1 h off). Specifically, two alginate types with low (LG) and high (HG) guluronic/mannuronic residue ratios with electrochemically synthesized silver nanoparticles (AgNPs) were evaluated. HG Ag/alginate hydrogels were clearly indicated as potential candidates due to better initial mechanical properties as compared to LG hydrogels (dynamic compression modulus of ~60 vs. ~40 kPa) as well as the mechanical stability displayed during 7 days of dynamic compression. Cytotoxicity studies in 3D bovine cartilage explant cultures under dynamic compression have shown negligible effects as compared to standard 2D monolayers of bovine chondrocytes where moderate cytotoxicity was observed. Finally, experimental and mathematical modeling studies revealed different mechanisms of AgNP release under physiological-like bioreactor conditions as compared to static conditions. Overall, the results clearly demonstrate bioreactor advantages in characterization and selection of candidate biomaterials as well as potentials to bridge the in vitro-in vivo gap.", publisher = "John Wiley and Sons Inc.", journal = "Journal of Biomedical Materials Research - Part A", title = "Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants", pages = "768-755", number = "4", volume = "107", doi = "10.1002/jbm.a.36590" }
Zvicer, J., Mišković-Stanković, V.,& Obradović, B.. (2019). Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants. in Journal of Biomedical Materials Research - Part A John Wiley and Sons Inc.., 107(4), 755-768. https://doi.org/10.1002/jbm.a.36590
Zvicer J, Mišković-Stanković V, Obradović B. Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants. in Journal of Biomedical Materials Research - Part A. 2019;107(4):755-768. doi:10.1002/jbm.a.36590 .
Zvicer, Jovana, Mišković-Stanković, Vesna, Obradović, Bojana, "Functional bioreactor characterization to assess potentials of nanocomposites based on different alginate types and silver nanoparticles for use as cartilage tissue implants" in Journal of Biomedical Materials Research - Part A, 107, no. 4 (2019):755-768, https://doi.org/10.1002/jbm.a.36590 . .