Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis
Autori
Mandić, MarijaMitić, Katarina
Nedeljković, Predrag
Perić, Mina
Božić, Bojan
Lunić, Tanja
Bačić, Ana
Rajilić-Stojanović, Mirjana
Peković, Sanja
Nedeljković Božić, Biljana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12—VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC t...reatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.
Ključne reči:
Experimental autoimmune encephalomyelitis / Gut microbiota / Nerve/muscle nuclear density / Neuroprotection / Popliteal lymph nodes / Vitamin B complexIzvor:
Nutrients, 2022, 14, 6, 1273-Izdavač:
- MDPI
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200178 (Univerzitet u Beogradu, Biološki fakultet) (RS-MESTD-inst-2020-200178)
DOI: 10.3390/nu14061273
ISSN: 2072-6643
WoS: 000774457300001
Scopus: 2-s2.0-85126446654
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Mandić, Marija AU - Mitić, Katarina AU - Nedeljković, Predrag AU - Perić, Mina AU - Božić, Bojan AU - Lunić, Tanja AU - Bačić, Ana AU - Rajilić-Stojanović, Mirjana AU - Peković, Sanja AU - Nedeljković Božić, Biljana PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5097 AB - The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12—VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE. PB - MDPI T2 - Nutrients T1 - Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis IS - 6 SP - 1273 VL - 14 DO - 10.3390/nu14061273 ER -
@article{ author = "Mandić, Marija and Mitić, Katarina and Nedeljković, Predrag and Perić, Mina and Božić, Bojan and Lunić, Tanja and Bačić, Ana and Rajilić-Stojanović, Mirjana and Peković, Sanja and Nedeljković Božić, Biljana", year = "2022", abstract = "The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12—VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.", publisher = "MDPI", journal = "Nutrients", title = "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis", number = "6", pages = "1273", volume = "14", doi = "10.3390/nu14061273" }
Mandić, M., Mitić, K., Nedeljković, P., Perić, M., Božić, B., Lunić, T., Bačić, A., Rajilić-Stojanović, M., Peković, S.,& Nedeljković Božić, B.. (2022). Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis. in Nutrients MDPI., 14(6), 1273. https://doi.org/10.3390/nu14061273
Mandić M, Mitić K, Nedeljković P, Perić M, Božić B, Lunić T, Bačić A, Rajilić-Stojanović M, Peković S, Nedeljković Božić B. Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis. in Nutrients. 2022;14(6):1273. doi:10.3390/nu14061273 .
Mandić, Marija, Mitić, Katarina, Nedeljković, Predrag, Perić, Mina, Božić, Bojan, Lunić, Tanja, Bačić, Ana, Rajilić-Stojanović, Mirjana, Peković, Sanja, Nedeljković Božić, Biljana, "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis" in Nutrients, 14, no. 6 (2022):1273, https://doi.org/10.3390/nu14061273 . .