Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage
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This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses stillage. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g-1 substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g-1 substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 °C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differ...ed depending on the enzyme type (for laccase it was <5 %, manganese peroxidase ≈12 %, cellulase ≈21 % and xylanase ≈16 %), which should be taken into consideration when optimizing the desired process.
Keywords:
biomass / enzymes / fungi / genetic algorithm / response surface methodSource:
Journal of the Serbian Chemical Society, 2022, 87, 4, 437-450Publisher:
- Serbian Chemical Society
Funding / projects:
- The Research presented in this paper was funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Project #I-1). It is a part of Scientific and Technological Collaboration of Republic of Serbia and the People’s Republic of China
DOI: 10.2298/JSC200601075J
ISSN: 0352-5139
WoS: 000720102300001
Scopus: 2-s2.0-85133977154
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Jović, Jelena AU - Hao, Jian AU - Mojović, Ljiljana PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5182 AB - This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses stillage. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g-1 substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g-1 substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 °C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was <5 %, manganese peroxidase ≈12 %, cellulase ≈21 % and xylanase ≈16 %), which should be taken into consideration when optimizing the desired process. PB - Serbian Chemical Society T2 - Journal of the Serbian Chemical Society T1 - Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage EP - 450 IS - 4 SP - 437 VL - 87 DO - 10.2298/JSC200601075J ER -
@article{ author = "Jović, Jelena and Hao, Jian and Mojović, Ljiljana", year = "2022", abstract = "This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses stillage. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g-1 substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g-1 substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 °C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was <5 %, manganese peroxidase ≈12 %, cellulase ≈21 % and xylanase ≈16 %), which should be taken into consideration when optimizing the desired process.", publisher = "Serbian Chemical Society", journal = "Journal of the Serbian Chemical Society", title = "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage", pages = "450-437", number = "4", volume = "87", doi = "10.2298/JSC200601075J" }
Jović, J., Hao, J.,& Mojović, L.. (2022). Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society Serbian Chemical Society., 87(4), 437-450. https://doi.org/10.2298/JSC200601075J
Jović J, Hao J, Mojović L. Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society. 2022;87(4):437-450. doi:10.2298/JSC200601075J .
Jović, Jelena, Hao, Jian, Mojović, Ljiljana, "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage" in Journal of the Serbian Chemical Society, 87, no. 4 (2022):437-450, https://doi.org/10.2298/JSC200601075J . .