Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides
Samo za registrovane korisnike
2022
Autori
Veljković, MilicaSimović, Milica
Banjanac, Katarina
Ćorović, Marija
Milivojević, Ana
Milivojević, Milan
Bezbradica, Dejan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study, a heterofunctional carrier was obtained by modification of a macroporous polystyrene ion exchange resin with primary amino groups (Purolite® A109) with epichlorohydrin, in view of achieving covalent immobilization of fructosyltransferase (FTase) from a complex enzyme mixture, Pectinex® Ultra SP-L, that is responsible for the synthesis of functionally active fructo-oligosaccharides (FOS). A two-step immobilization protocol, comprising the physical adsorption of FTase performed at pH 4 and subsequent buffer exchange to promote the establishment of covalent bonds at pH 9, was proposed for obtaining a highly active immobilized preparation (243 IU g−1 of support). Additionally, this protocol provided development of a preparation with 20 times more prominent expressed activity of FTase compared to the commercial preparation with predominant pectinase activity. The obtained immobilized preparation was further tested in batch and air-lift reactor systems for FOS synthesis, yield...ing 52.8% and 54.7% of FOS in total carbohydrates, respectively. Finally, the continuous production of FOS in the air-lift reactor was established for 7 days, with an average FOS yield of 52.5%. Accordingly, it is demonstrated that the immobilization process enabled the development of preparations with exceptional potential for industrial implementation.
Izvor:
Reaction Chemistry and Engineering, 2022, 7, 12, 2518-2526Izdavač:
- Royal Society of Chemistry
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200287 (Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (RS-MESTD-inst-2020-200287)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-MESTD-inst-2020-200135)
- PrIntPrEnzy - Prebiotics for functional food and bioactive cosmetics produced in intensified enzymatic processes (RS-ScienceFundRS-Ideje-7750109)
DOI: 10.1039/d2re00182a
ISSN: 2058-9883
WoS: 00084465910000
Scopus: 2-s2.0-85143631792
Kolekcije
Institucija/grupa
Inovacioni centarTY - JOUR AU - Veljković, Milica AU - Simović, Milica AU - Banjanac, Katarina AU - Ćorović, Marija AU - Milivojević, Ana AU - Milivojević, Milan AU - Bezbradica, Dejan PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5281 AB - In this study, a heterofunctional carrier was obtained by modification of a macroporous polystyrene ion exchange resin with primary amino groups (Purolite® A109) with epichlorohydrin, in view of achieving covalent immobilization of fructosyltransferase (FTase) from a complex enzyme mixture, Pectinex® Ultra SP-L, that is responsible for the synthesis of functionally active fructo-oligosaccharides (FOS). A two-step immobilization protocol, comprising the physical adsorption of FTase performed at pH 4 and subsequent buffer exchange to promote the establishment of covalent bonds at pH 9, was proposed for obtaining a highly active immobilized preparation (243 IU g−1 of support). Additionally, this protocol provided development of a preparation with 20 times more prominent expressed activity of FTase compared to the commercial preparation with predominant pectinase activity. The obtained immobilized preparation was further tested in batch and air-lift reactor systems for FOS synthesis, yielding 52.8% and 54.7% of FOS in total carbohydrates, respectively. Finally, the continuous production of FOS in the air-lift reactor was established for 7 days, with an average FOS yield of 52.5%. Accordingly, it is demonstrated that the immobilization process enabled the development of preparations with exceptional potential for industrial implementation. PB - Royal Society of Chemistry T2 - Reaction Chemistry and Engineering T1 - Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides EP - 2526 IS - 12 SP - 2518 VL - 7 DO - 10.1039/d2re00182a ER -
@article{ author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Milivojević, Milan and Bezbradica, Dejan", year = "2022", abstract = "In this study, a heterofunctional carrier was obtained by modification of a macroporous polystyrene ion exchange resin with primary amino groups (Purolite® A109) with epichlorohydrin, in view of achieving covalent immobilization of fructosyltransferase (FTase) from a complex enzyme mixture, Pectinex® Ultra SP-L, that is responsible for the synthesis of functionally active fructo-oligosaccharides (FOS). A two-step immobilization protocol, comprising the physical adsorption of FTase performed at pH 4 and subsequent buffer exchange to promote the establishment of covalent bonds at pH 9, was proposed for obtaining a highly active immobilized preparation (243 IU g−1 of support). Additionally, this protocol provided development of a preparation with 20 times more prominent expressed activity of FTase compared to the commercial preparation with predominant pectinase activity. The obtained immobilized preparation was further tested in batch and air-lift reactor systems for FOS synthesis, yielding 52.8% and 54.7% of FOS in total carbohydrates, respectively. Finally, the continuous production of FOS in the air-lift reactor was established for 7 days, with an average FOS yield of 52.5%. Accordingly, it is demonstrated that the immobilization process enabled the development of preparations with exceptional potential for industrial implementation.", publisher = "Royal Society of Chemistry", journal = "Reaction Chemistry and Engineering", title = "Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides", pages = "2526-2518", number = "12", volume = "7", doi = "10.1039/d2re00182a" }
Veljković, M., Simović, M., Banjanac, K., Ćorović, M., Milivojević, A., Milivojević, M.,& Bezbradica, D.. (2022). Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides. in Reaction Chemistry and Engineering Royal Society of Chemistry., 7(12), 2518-2526. https://doi.org/10.1039/d2re00182a
Veljković M, Simović M, Banjanac K, Ćorović M, Milivojević A, Milivojević M, Bezbradica D. Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides. in Reaction Chemistry and Engineering. 2022;7(12):2518-2526. doi:10.1039/d2re00182a .
Veljković, Milica, Simović, Milica, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Milivojević, Milan, Bezbradica, Dejan, "Heterofunctional epoxy support development for immobilization of fructosyltransferase from Pectinex® Ultra SP-L: batch and continuous production of fructo-oligosaccharides" in Reaction Chemistry and Engineering, 7, no. 12 (2022):2518-2526, https://doi.org/10.1039/d2re00182a . .