Stabilization of alpha-glucosidase in organic solvents by immobilization on macroporous poly(GMA-co-EGDMA) with different surface characteristics
Stabilizacija α-glukozidaze u organskim rastvaračima imobilizacijom na makroporoznim (poli) glicidil metakrilatima različitih površinskih karakteristika
Autori
Prodanović, RadivojeMilosavic, Nenad B.
Jovanović, Slobodan
Ćirković-Veličković, Tanja
Vujčić, Zoran
Jankov, Ratko M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
alpha-Glucosidase from baker's yeast was immobilized on macroporous copolymers of ethylene glycol dimethacrylate and glycidyl methacrylate, poly(GMA-co-EGDMA), with various surface characteristics and pore sizes ranging from 44 nm to 270 nm. Immobilization was done by glutaraldehyde on the copolymer previously modified with 1,2-diaminoethane. The specific activity of the obtained immobilized enzyme varied from 27 to 81 U/g depending on the employed copolymer. The half lives of the immobilized enzyme in cosolvents were influenced by the surface characteristics of the copolymer, ranging from 60 to 150 min in 35 % methanol and from 10 to 44 min in 45 % dimethyl sulphoxide (DMSO). The best stabilities were obtained when the enzyme was immobilized onto a copolymer having a pore size of 48 rim in methanol and 270 nm in DMSO.
α-Glukozidaza izolovana iz pekarskog kvasca je imobilizovana na makroporoznim glicidil-metakrilatima različitih površinskih karakteristika i veličina pora od 44 do 270 nm. Imobilizacija je izvedena glutaraldehidom na polimeru prethodno modifikovanom sa 1,2-diaminoetanom. Specifična aktivnost dobijenog imobilizovanog enzima je varirala od 27 do 81U/g u zavisnosti od vrste korišćenog polimera. Poluživoti imobilizovanog enzima u korastvaračima su zavisili od površinskih karakteristika polimera i kretali su se u opsegu od 60 do 150 min u 35%(v/v) metanolu i od 10 do 44 min u 45 % (v/v) dimetilsufoksidu. Najveća stabilnost u metanolu je dobijena imobilizacijom enzima na polimeru sa veličinom pora od 48 nm a u dimetilsulfoksidu na polimeru sa veličinom pora od 270 nm.
Ključne reči:
cosolvent / eupergit / eupergit / maltase / maltase / immobilized / immobilized / stability / stabilityIzvor:
Journal of the Serbian Chemical Society, 2006, 71, 4, 339-347Izdavač:
- Serbian Chemical Soc, Belgrade
DOI: 10.2298/JSC0604339P
ISSN: 0352-5139
WoS: 000238365500003
Scopus: 2-s2.0-33746433457
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Prodanović, Radivoje AU - Milosavic, Nenad B. AU - Jovanović, Slobodan AU - Ćirković-Veličković, Tanja AU - Vujčić, Zoran AU - Jankov, Ratko M. PY - 2006 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5458 AB - alpha-Glucosidase from baker's yeast was immobilized on macroporous copolymers of ethylene glycol dimethacrylate and glycidyl methacrylate, poly(GMA-co-EGDMA), with various surface characteristics and pore sizes ranging from 44 nm to 270 nm. Immobilization was done by glutaraldehyde on the copolymer previously modified with 1,2-diaminoethane. The specific activity of the obtained immobilized enzyme varied from 27 to 81 U/g depending on the employed copolymer. The half lives of the immobilized enzyme in cosolvents were influenced by the surface characteristics of the copolymer, ranging from 60 to 150 min in 35 % methanol and from 10 to 44 min in 45 % dimethyl sulphoxide (DMSO). The best stabilities were obtained when the enzyme was immobilized onto a copolymer having a pore size of 48 rim in methanol and 270 nm in DMSO. AB - α-Glukozidaza izolovana iz pekarskog kvasca je imobilizovana na makroporoznim glicidil-metakrilatima različitih površinskih karakteristika i veličina pora od 44 do 270 nm. Imobilizacija je izvedena glutaraldehidom na polimeru prethodno modifikovanom sa 1,2-diaminoetanom. Specifična aktivnost dobijenog imobilizovanog enzima je varirala od 27 do 81U/g u zavisnosti od vrste korišćenog polimera. Poluživoti imobilizovanog enzima u korastvaračima su zavisili od površinskih karakteristika polimera i kretali su se u opsegu od 60 do 150 min u 35%(v/v) metanolu i od 10 do 44 min u 45 % (v/v) dimetilsufoksidu. Najveća stabilnost u metanolu je dobijena imobilizacijom enzima na polimeru sa veličinom pora od 48 nm a u dimetilsulfoksidu na polimeru sa veličinom pora od 270 nm. PB - Serbian Chemical Soc, Belgrade T2 - Journal of the Serbian Chemical Society T1 - Stabilization of alpha-glucosidase in organic solvents by immobilization on macroporous poly(GMA-co-EGDMA) with different surface characteristics T1 - Stabilizacija α-glukozidaze u organskim rastvaračima imobilizacijom na makroporoznim (poli) glicidil metakrilatima različitih površinskih karakteristika EP - 347 IS - 4 SP - 339 VL - 71 DO - 10.2298/JSC0604339P ER -
@article{ author = "Prodanović, Radivoje and Milosavic, Nenad B. and Jovanović, Slobodan and Ćirković-Veličković, Tanja and Vujčić, Zoran and Jankov, Ratko M.", year = "2006", abstract = "alpha-Glucosidase from baker's yeast was immobilized on macroporous copolymers of ethylene glycol dimethacrylate and glycidyl methacrylate, poly(GMA-co-EGDMA), with various surface characteristics and pore sizes ranging from 44 nm to 270 nm. Immobilization was done by glutaraldehyde on the copolymer previously modified with 1,2-diaminoethane. The specific activity of the obtained immobilized enzyme varied from 27 to 81 U/g depending on the employed copolymer. The half lives of the immobilized enzyme in cosolvents were influenced by the surface characteristics of the copolymer, ranging from 60 to 150 min in 35 % methanol and from 10 to 44 min in 45 % dimethyl sulphoxide (DMSO). The best stabilities were obtained when the enzyme was immobilized onto a copolymer having a pore size of 48 rim in methanol and 270 nm in DMSO., α-Glukozidaza izolovana iz pekarskog kvasca je imobilizovana na makroporoznim glicidil-metakrilatima različitih površinskih karakteristika i veličina pora od 44 do 270 nm. Imobilizacija je izvedena glutaraldehidom na polimeru prethodno modifikovanom sa 1,2-diaminoetanom. Specifična aktivnost dobijenog imobilizovanog enzima je varirala od 27 do 81U/g u zavisnosti od vrste korišćenog polimera. Poluživoti imobilizovanog enzima u korastvaračima su zavisili od površinskih karakteristika polimera i kretali su se u opsegu od 60 do 150 min u 35%(v/v) metanolu i od 10 do 44 min u 45 % (v/v) dimetilsufoksidu. Najveća stabilnost u metanolu je dobijena imobilizacijom enzima na polimeru sa veličinom pora od 48 nm a u dimetilsulfoksidu na polimeru sa veličinom pora od 270 nm.", publisher = "Serbian Chemical Soc, Belgrade", journal = "Journal of the Serbian Chemical Society", title = "Stabilization of alpha-glucosidase in organic solvents by immobilization on macroporous poly(GMA-co-EGDMA) with different surface characteristics, Stabilizacija α-glukozidaze u organskim rastvaračima imobilizacijom na makroporoznim (poli) glicidil metakrilatima različitih površinskih karakteristika", pages = "347-339", number = "4", volume = "71", doi = "10.2298/JSC0604339P" }
Prodanović, R., Milosavic, N. B., Jovanović, S., Ćirković-Veličković, T., Vujčić, Z.,& Jankov, R. M.. (2006). Stabilization of alpha-glucosidase in organic solvents by immobilization on macroporous poly(GMA-co-EGDMA) with different surface characteristics. in Journal of the Serbian Chemical Society Serbian Chemical Soc, Belgrade., 71(4), 339-347. https://doi.org/10.2298/JSC0604339P
Prodanović R, Milosavic NB, Jovanović S, Ćirković-Veličković T, Vujčić Z, Jankov RM. Stabilization of alpha-glucosidase in organic solvents by immobilization on macroporous poly(GMA-co-EGDMA) with different surface characteristics. in Journal of the Serbian Chemical Society. 2006;71(4):339-347. doi:10.2298/JSC0604339P .
Prodanović, Radivoje, Milosavic, Nenad B., Jovanović, Slobodan, Ćirković-Veličković, Tanja, Vujčić, Zoran, Jankov, Ratko M., "Stabilization of alpha-glucosidase in organic solvents by immobilization on macroporous poly(GMA-co-EGDMA) with different surface characteristics" in Journal of the Serbian Chemical Society, 71, no. 4 (2006):339-347, https://doi.org/10.2298/JSC0604339P . .