Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
Authorized Users Only
2013
Authors
Fanovich, M.A.Ivanović, J.
Mišić, D.
Alvarez, M.V.
Jaeger, P.
Žižović, Irena
Eggers, Rudolf
Article (Published version)
Metadata
Show full item recordAbstract
The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon ...dioxide at 35 C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed.
Keywords:
Antibacterial properties / Polycaprolactone scaffold / Supercritical extraction impregnation / Usnea extractSource:
Journal of Supercritical Fluids, 2013, 78, 42-53Publisher:
- Elsevier
Funding / projects:
- Functional physiologically active plant materials with additional values for application in pharmaceutical and food industry (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45017)
DOI: 10.1016/j.supflu.2013.03.017
ISSN: 0896-8446
WoS: 000319854000007
Scopus: 2-s2.0-84876222754
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Fanovich, M.A. AU - Ivanović, J. AU - Mišić, D. AU - Alvarez, M.V. AU - Jaeger, P. AU - Žižović, Irena AU - Eggers, Rudolf PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5698 AB - The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon dioxide at 35 C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed. PB - Elsevier T2 - Journal of Supercritical Fluids T1 - Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process EP - 53 SP - 42 VL - 78 DO - 10.1016/j.supflu.2013.03.017 ER -
@article{ author = "Fanovich, M.A. and Ivanović, J. and Mišić, D. and Alvarez, M.V. and Jaeger, P. and Žižović, Irena and Eggers, Rudolf", year = "2013", abstract = "The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 μg/mL to 320 μg/mL against Listeria innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 μm can be obtained when PCL is exposed to carbon dioxide at 35 C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L. innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 × 104 Colony-forming Units/mL [CFU/mL]) was assayed.", publisher = "Elsevier", journal = "Journal of Supercritical Fluids", title = "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process", pages = "53-42", volume = "78", doi = "10.1016/j.supflu.2013.03.017" }
Fanovich, M.A., Ivanović, J., Mišić, D., Alvarez, M.V., Jaeger, P., Žižović, I.,& Eggers, R.. (2013). Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids Elsevier., 78, 42-53. https://doi.org/10.1016/j.supflu.2013.03.017
Fanovich M, Ivanović J, Mišić D, Alvarez M, Jaeger P, Žižović I, Eggers R. Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids. 2013;78:42-53. doi:10.1016/j.supflu.2013.03.017 .
Fanovich, M.A., Ivanović, J., Mišić, D., Alvarez, M.V., Jaeger, P., Žižović, Irena, Eggers, Rudolf, "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process" in Journal of Supercritical Fluids, 78 (2013):42-53, https://doi.org/10.1016/j.supflu.2013.03.017 . .