Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves
Autori
Mijalković, JelenaŠekuljica, Nataša
Biçer, Furkan
Pavlović, Neda
Jakovetić Tanasković, Sonja
Knežević-Jugović, Zorica
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Proteins are an essential component of the human diet and effectively are provided from
both animal and plant sources. Sourcing ribulose-1,5-bisphosphate carboxylase/oxygenase
(RuBisCO) from green biomass such as pumpkin leaves, a by-product of the oil industry,
is a promising tool for obtaining protein with an excellent nutritional profile under the
green biorafinery concept, that can be used as a substitute for animal proteins. This study
provides the results regarding the implementation of ultrasound-assisted processing (UAP)
in the protein extraction from pumpkin leaves. UAP for recovery of RuBisCO proteins was
optimized by varing the solvent-to-sample ratio, solvent pH, ultrasound amplitudes and
time. UAP appears to have a strong potential for recovering RuBisCO protein from
pumpkin leaves, as it was highly influenced by the solvent-to-sample ratio along with UAP
amplitude and time.
Proteini životinjskog i biljnog porekla čine osnovne namirnice ljudske ishrane. Prema
konceptu zelene biorafinerije, izolovanje RuBisCO proteina (ribuloza-1,5-bisfosfat
karboksilaza/oksigenaze) iz zelene biomase kakva je lišće bundeve, nusproizvod industrije
prerade uljarica, je obećavajući pristup za dobijanje proteina sa visokom nutritivnom
vrednošću koje je moguće koristiti kao zamenu za proteine životinjskog porekla. U ovom
radu, ultrazvučno potpomognutim procesom ekstrakcije izolovani su proteini iz lišća
bundeve. Ultrazvučni proces ekstrakcije RuBisCO proteina optimizovan je variranjem
odnosa rastvarač-uzorak, pH rastvarača, vremena trajanja i amplitude ultrazvuka.
Ultrazvuk je ispoljio veliki pozitivan efekat na izolovanje RuBisCO proteina iz lišća
bundeve, na šta je u najvećoj meri tokom optimizacije procesa uticao odnos rastvaračuzorak, kao i amplituda i vreme ultrazvuka.
Ključne reči:
Ultrasound-assisted extraction / Plant-based protein / RuBisCO / Pumpkin leaves / Extraction optimizationIzvor:
Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade, 2022, 174-174Izdavač:
- Beograd : Srpsko hemijsko društvo = Serbian Chemical Society
Finansiranje / projekti:
- MultiPromis - Multifunctional leaf protein and assembled nanocarrier structures delivered by enzyme technology (RS-ScienceFundRS-Ideje-7751519)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-MESTD-inst-2020-200135)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200287 (Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (RS-MESTD-inst-2020-200287)
Kolekcije
Institucija/grupa
Tehnološko-metalurški fakultetTY - CONF AU - Mijalković, Jelena AU - Šekuljica, Nataša AU - Biçer, Furkan AU - Pavlović, Neda AU - Jakovetić Tanasković, Sonja AU - Knežević-Jugović, Zorica PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6252 AB - Proteins are an essential component of the human diet and effectively are provided from both animal and plant sources. Sourcing ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from green biomass such as pumpkin leaves, a by-product of the oil industry, is a promising tool for obtaining protein with an excellent nutritional profile under the green biorafinery concept, that can be used as a substitute for animal proteins. This study provides the results regarding the implementation of ultrasound-assisted processing (UAP) in the protein extraction from pumpkin leaves. UAP for recovery of RuBisCO proteins was optimized by varing the solvent-to-sample ratio, solvent pH, ultrasound amplitudes and time. UAP appears to have a strong potential for recovering RuBisCO protein from pumpkin leaves, as it was highly influenced by the solvent-to-sample ratio along with UAP amplitude and time. AB - Proteini životinjskog i biljnog porekla čine osnovne namirnice ljudske ishrane. Prema konceptu zelene biorafinerije, izolovanje RuBisCO proteina (ribuloza-1,5-bisfosfat karboksilaza/oksigenaze) iz zelene biomase kakva je lišće bundeve, nusproizvod industrije prerade uljarica, je obećavajući pristup za dobijanje proteina sa visokom nutritivnom vrednošću koje je moguće koristiti kao zamenu za proteine životinjskog porekla. U ovom radu, ultrazvučno potpomognutim procesom ekstrakcije izolovani su proteini iz lišća bundeve. Ultrazvučni proces ekstrakcije RuBisCO proteina optimizovan je variranjem odnosa rastvarač-uzorak, pH rastvarača, vremena trajanja i amplitude ultrazvuka. Ultrazvuk je ispoljio veliki pozitivan efekat na izolovanje RuBisCO proteina iz lišća bundeve, na šta je u najvećoj meri tokom optimizacije procesa uticao odnos rastvaračuzorak, kao i amplituda i vreme ultrazvuka. PB - Beograd : Srpsko hemijsko društvo = Serbian Chemical Society C3 - Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade T1 - Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves EP - 174 SP - 174 UR - https://hdl.handle.net/21.15107/rcub_technorep_6252 ER -
@conference{ author = "Mijalković, Jelena and Šekuljica, Nataša and Biçer, Furkan and Pavlović, Neda and Jakovetić Tanasković, Sonja and Knežević-Jugović, Zorica", year = "2022", abstract = "Proteins are an essential component of the human diet and effectively are provided from both animal and plant sources. Sourcing ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from green biomass such as pumpkin leaves, a by-product of the oil industry, is a promising tool for obtaining protein with an excellent nutritional profile under the green biorafinery concept, that can be used as a substitute for animal proteins. This study provides the results regarding the implementation of ultrasound-assisted processing (UAP) in the protein extraction from pumpkin leaves. UAP for recovery of RuBisCO proteins was optimized by varing the solvent-to-sample ratio, solvent pH, ultrasound amplitudes and time. UAP appears to have a strong potential for recovering RuBisCO protein from pumpkin leaves, as it was highly influenced by the solvent-to-sample ratio along with UAP amplitude and time., Proteini životinjskog i biljnog porekla čine osnovne namirnice ljudske ishrane. Prema konceptu zelene biorafinerije, izolovanje RuBisCO proteina (ribuloza-1,5-bisfosfat karboksilaza/oksigenaze) iz zelene biomase kakva je lišće bundeve, nusproizvod industrije prerade uljarica, je obećavajući pristup za dobijanje proteina sa visokom nutritivnom vrednošću koje je moguće koristiti kao zamenu za proteine životinjskog porekla. U ovom radu, ultrazvučno potpomognutim procesom ekstrakcije izolovani su proteini iz lišća bundeve. Ultrazvučni proces ekstrakcije RuBisCO proteina optimizovan je variranjem odnosa rastvarač-uzorak, pH rastvarača, vremena trajanja i amplitude ultrazvuka. Ultrazvuk je ispoljio veliki pozitivan efekat na izolovanje RuBisCO proteina iz lišća bundeve, na šta je u najvećoj meri tokom optimizacije procesa uticao odnos rastvaračuzorak, kao i amplituda i vreme ultrazvuka.", publisher = "Beograd : Srpsko hemijsko društvo = Serbian Chemical Society", journal = "Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade", title = "Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves", pages = "174-174", url = "https://hdl.handle.net/21.15107/rcub_technorep_6252" }
Mijalković, J., Šekuljica, N., Biçer, F., Pavlović, N., Jakovetić Tanasković, S.,& Knežević-Jugović, Z.. (2022). Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves. in Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade Beograd : Srpsko hemijsko društvo = Serbian Chemical Society., 174-174. https://hdl.handle.net/21.15107/rcub_technorep_6252
Mijalković J, Šekuljica N, Biçer F, Pavlović N, Jakovetić Tanasković S, Knežević-Jugović Z. Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves. in Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade. 2022;:174-174. https://hdl.handle.net/21.15107/rcub_technorep_6252 .
Mijalković, Jelena, Šekuljica, Nataša, Biçer, Furkan, Pavlović, Neda, Jakovetić Tanasković, Sonja, Knežević-Jugović, Zorica, "Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves" in Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade (2022):174-174, https://hdl.handle.net/21.15107/rcub_technorep_6252 .