Peptides with improved antimicrobial activity screened by membrane ultrafiltration from egg white protein hydrolysates
Authors
Jovanović, JelenaStefanović, Andrea
Grbavčić, Sanja
Šekuljica, Nataša
Elmalimadi, Mohamed B.
Bugarski, Branko
Knežević-Jugović, Zorica
Conference object (Published version)
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This contribution was aimed at the fractionation and identification of peptides with improved
antimicrobial activity from egg white protein hydrolysates, obtained by membrane ultrafiltration. For
this purpose, the thermal treated egg white proteins were intensively hydrolysed with a commercial
food-grade bacterial endopeptidase from Bacillus licheniformis, namely Alcalase. Thus, obtained
hydrolysates were further separated by sequential ultrafiltration into four peptide fractions viz. fraction I (> 30kDa), II (10 - 30 kDa), III (1 - 10 kDa) and IV (< 1kDa) which were investigated in terms of their antimicrobial activity. The antimicrobial activity was tested against Gram-positive bacteria (Staphylococcus aureus ATCC 25923), Gram-negative bacteria (Escherichia coli ATCC 25922) and against yeast Candida albicans (ATCC 24433) by fractions' susceptibility of agar diffusion. Our results showed that these peptide fractions have an intense inhibitory activity on Gram-positive bacteria, po...or on Gram-negative bacteria and none inhibitory activity on growth of C. albicans. The results showed interesting antimicrobial potentials versus the tested microorganisms, especially fractions with peptides molecular weight of 10-30 kDa and 1-10 kDa. It can be concluded, that the controlled enzymatic hydrolysis of egg white proteins and their subsequent membrane ultrafiltration is considered to be a suitable way for production of biocative peptides with exhibit antimicrobial efficiency.
Keywords:
Antimicrobial derived egg white peptides / Radial diffusion assay / Membrane ultrafiltration / Hydrolysis / AlcalaseSource:
Proceedings / 42nd International Conference of Slovak Society of Chemical Engineering, 2015, 732-739Publisher:
- Bratislava, SK : Slovak Society of Chemical Engineering
Funding / projects:
- EUREKA project / ENZEGG E!6750 - Development of enzyme processes for production of egg white protein hydrolysates
- Innovation Project / Production of new dietary formulations based on natural protein with antioxidant and antitumor effect
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
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Institution/Community
Tehnološko-metalurški fakultetTY - CONF AU - Jovanović, Jelena AU - Stefanović, Andrea AU - Grbavčić, Sanja AU - Šekuljica, Nataša AU - Elmalimadi, Mohamed B. AU - Bugarski, Branko AU - Knežević-Jugović, Zorica PY - 2015 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6275 AB - This contribution was aimed at the fractionation and identification of peptides with improved antimicrobial activity from egg white protein hydrolysates, obtained by membrane ultrafiltration. For this purpose, the thermal treated egg white proteins were intensively hydrolysed with a commercial food-grade bacterial endopeptidase from Bacillus licheniformis, namely Alcalase. Thus, obtained hydrolysates were further separated by sequential ultrafiltration into four peptide fractions viz. fraction I (> 30kDa), II (10 - 30 kDa), III (1 - 10 kDa) and IV (< 1kDa) which were investigated in terms of their antimicrobial activity. The antimicrobial activity was tested against Gram-positive bacteria (Staphylococcus aureus ATCC 25923), Gram-negative bacteria (Escherichia coli ATCC 25922) and against yeast Candida albicans (ATCC 24433) by fractions' susceptibility of agar diffusion. Our results showed that these peptide fractions have an intense inhibitory activity on Gram-positive bacteria, poor on Gram-negative bacteria and none inhibitory activity on growth of C. albicans. The results showed interesting antimicrobial potentials versus the tested microorganisms, especially fractions with peptides molecular weight of 10-30 kDa and 1-10 kDa. It can be concluded, that the controlled enzymatic hydrolysis of egg white proteins and their subsequent membrane ultrafiltration is considered to be a suitable way for production of biocative peptides with exhibit antimicrobial efficiency. PB - Bratislava, SK : Slovak Society of Chemical Engineering C3 - Proceedings / 42nd International Conference of Slovak Society of Chemical Engineering T1 - Peptides with improved antimicrobial activity screened by membrane ultrafiltration from egg white protein hydrolysates EP - 739 SP - 732 UR - https://hdl.handle.net/21.15107/rcub_technorep_6275 ER -
@conference{ author = "Jovanović, Jelena and Stefanović, Andrea and Grbavčić, Sanja and Šekuljica, Nataša and Elmalimadi, Mohamed B. and Bugarski, Branko and Knežević-Jugović, Zorica", year = "2015", abstract = "This contribution was aimed at the fractionation and identification of peptides with improved antimicrobial activity from egg white protein hydrolysates, obtained by membrane ultrafiltration. For this purpose, the thermal treated egg white proteins were intensively hydrolysed with a commercial food-grade bacterial endopeptidase from Bacillus licheniformis, namely Alcalase. Thus, obtained hydrolysates were further separated by sequential ultrafiltration into four peptide fractions viz. fraction I (> 30kDa), II (10 - 30 kDa), III (1 - 10 kDa) and IV (< 1kDa) which were investigated in terms of their antimicrobial activity. The antimicrobial activity was tested against Gram-positive bacteria (Staphylococcus aureus ATCC 25923), Gram-negative bacteria (Escherichia coli ATCC 25922) and against yeast Candida albicans (ATCC 24433) by fractions' susceptibility of agar diffusion. Our results showed that these peptide fractions have an intense inhibitory activity on Gram-positive bacteria, poor on Gram-negative bacteria and none inhibitory activity on growth of C. albicans. The results showed interesting antimicrobial potentials versus the tested microorganisms, especially fractions with peptides molecular weight of 10-30 kDa and 1-10 kDa. It can be concluded, that the controlled enzymatic hydrolysis of egg white proteins and their subsequent membrane ultrafiltration is considered to be a suitable way for production of biocative peptides with exhibit antimicrobial efficiency.", publisher = "Bratislava, SK : Slovak Society of Chemical Engineering", journal = "Proceedings / 42nd International Conference of Slovak Society of Chemical Engineering", title = "Peptides with improved antimicrobial activity screened by membrane ultrafiltration from egg white protein hydrolysates", pages = "739-732", url = "https://hdl.handle.net/21.15107/rcub_technorep_6275" }
Jovanović, J., Stefanović, A., Grbavčić, S., Šekuljica, N., Elmalimadi, M. B., Bugarski, B.,& Knežević-Jugović, Z.. (2015). Peptides with improved antimicrobial activity screened by membrane ultrafiltration from egg white protein hydrolysates. in Proceedings / 42nd International Conference of Slovak Society of Chemical Engineering Bratislava, SK : Slovak Society of Chemical Engineering., 732-739. https://hdl.handle.net/21.15107/rcub_technorep_6275
Jovanović J, Stefanović A, Grbavčić S, Šekuljica N, Elmalimadi MB, Bugarski B, Knežević-Jugović Z. Peptides with improved antimicrobial activity screened by membrane ultrafiltration from egg white protein hydrolysates. in Proceedings / 42nd International Conference of Slovak Society of Chemical Engineering. 2015;:732-739. https://hdl.handle.net/21.15107/rcub_technorep_6275 .
Jovanović, Jelena, Stefanović, Andrea, Grbavčić, Sanja, Šekuljica, Nataša, Elmalimadi, Mohamed B., Bugarski, Branko, Knežević-Jugović, Zorica, "Peptides with improved antimicrobial activity screened by membrane ultrafiltration from egg white protein hydrolysates" in Proceedings / 42nd International Conference of Slovak Society of Chemical Engineering (2015):732-739, https://hdl.handle.net/21.15107/rcub_technorep_6275 .