Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti
Authors
Buntić, AnetaStajković-Srbinović, Olivera
Milić, Marija
Dubljanin, Tijana
Kuzmanović, Đorđe
Knežević, Magdalena
Delić, Dušica
Conference object (Published version)
Metadata
Show full item recordAbstract
Miscanthus × giganteus, as a lignocellulosic material, consists of about 30%
xylan and represents a good basis for research as a possible raw material in
the production of xylanases. Among the soil bacteria, rhizobium is rarely
investigated as an enzyme producer. For the first time, Sinorhizobium
meliloti strain 207 was used to obtain xylanase during submerged and solid
fermentation using miscanthus biomass as a substrate. During submerged
fermentation (28°C), the maximum xylanase activity was achieved after 48 h
with 10% inoculum and H2SO4 substrate modification. The maximum
xylanase activity of 1.215 U/mL was obtained during solid-state
fermentation (28°C) by using also H2SO4 modified miscanthus biomass
which was moistened with distillate water. The crude enzyme, produced by
strain 207, could be further used in eco-friendly processes of lignocellulose
material bioconversion to useful products.
Keywords:
agroindustrial waste / xylanase activity / solid-state fermentation / Sinorhizobium melilotiSource:
1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection, 2019, 151-163Publisher:
- The Balkans Scientific Center of the Russian Academy of Natural Sciences
Funding / projects:
- Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-MESTD-Technological Development (TD or TR)-31035)
- Study of the effects of soil and irrigation water quality on more efficient agricultural crop production and environment protection (RS-MESTD-Technological Development (TD or TR)-37006)
Institution/Community
Tehnološko-metalurški fakultetTY - CONF AU - Buntić, Aneta AU - Stajković-Srbinović, Olivera AU - Milić, Marija AU - Dubljanin, Tijana AU - Kuzmanović, Đorđe AU - Knežević, Magdalena AU - Delić, Dušica PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6415 AB - Miscanthus × giganteus, as a lignocellulosic material, consists of about 30% xylan and represents a good basis for research as a possible raw material in the production of xylanases. Among the soil bacteria, rhizobium is rarely investigated as an enzyme producer. For the first time, Sinorhizobium meliloti strain 207 was used to obtain xylanase during submerged and solid fermentation using miscanthus biomass as a substrate. During submerged fermentation (28°C), the maximum xylanase activity was achieved after 48 h with 10% inoculum and H2SO4 substrate modification. The maximum xylanase activity of 1.215 U/mL was obtained during solid-state fermentation (28°C) by using also H2SO4 modified miscanthus biomass which was moistened with distillate water. The crude enzyme, produced by strain 207, could be further used in eco-friendly processes of lignocellulose material bioconversion to useful products. PB - The Balkans Scientific Center of the Russian Academy of Natural Sciences C3 - 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection T1 - Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti EP - 163 SP - 151 UR - https://hdl.handle.net/21.15107/rcub_technorep_6415 ER -
@conference{ author = "Buntić, Aneta and Stajković-Srbinović, Olivera and Milić, Marija and Dubljanin, Tijana and Kuzmanović, Đorđe and Knežević, Magdalena and Delić, Dušica", year = "2019", abstract = "Miscanthus × giganteus, as a lignocellulosic material, consists of about 30% xylan and represents a good basis for research as a possible raw material in the production of xylanases. Among the soil bacteria, rhizobium is rarely investigated as an enzyme producer. For the first time, Sinorhizobium meliloti strain 207 was used to obtain xylanase during submerged and solid fermentation using miscanthus biomass as a substrate. During submerged fermentation (28°C), the maximum xylanase activity was achieved after 48 h with 10% inoculum and H2SO4 substrate modification. The maximum xylanase activity of 1.215 U/mL was obtained during solid-state fermentation (28°C) by using also H2SO4 modified miscanthus biomass which was moistened with distillate water. The crude enzyme, produced by strain 207, could be further used in eco-friendly processes of lignocellulose material bioconversion to useful products.", publisher = "The Balkans Scientific Center of the Russian Academy of Natural Sciences", journal = "1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection", title = "Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti", pages = "163-151", url = "https://hdl.handle.net/21.15107/rcub_technorep_6415" }
Buntić, A., Stajković-Srbinović, O., Milić, M., Dubljanin, T., Kuzmanović, Đ., Knežević, M.,& Delić, D.. (2019). Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti. in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection The Balkans Scientific Center of the Russian Academy of Natural Sciences., 151-163. https://hdl.handle.net/21.15107/rcub_technorep_6415
Buntić A, Stajković-Srbinović O, Milić M, Dubljanin T, Kuzmanović Đ, Knežević M, Delić D. Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti. in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection. 2019;:151-163. https://hdl.handle.net/21.15107/rcub_technorep_6415 .
Buntić, Aneta, Stajković-Srbinović, Olivera, Milić, Marija, Dubljanin, Tijana, Kuzmanović, Đorđe, Knežević, Magdalena, Delić, Dušica, "Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti" in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection (2019):151-163, https://hdl.handle.net/21.15107/rcub_technorep_6415 .