Protein-repellent and antioxidative properties of bioactive coatings based on TEMPO oxidized cellulose nanofibrils and chitosan
Protein-odbijajuća i antioksidativna svojstva bioaktivnih prevlaka na bazi TEMPO oksidisanih celuloznih nanofibrila i hitozana
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In this work, regenerated cellulose (RC) films was coated with
TEMPO oxidized cellulose nanofibrils (TOCN) and chitosan (CS) by means of
subsequent spin-coated deposition. The bioactivity of coatings was
achieved by addition of chitosan. The chitosan was either mixed
with the TOCN (TOCN+CS) and deposited on the RC film by spin-coating or
deposited on the RC/TOCN bilayer film by pumping its aqueous solution at
pH 5.5 over the surface of the film. The pH dependant charging behaviour
of the TOCN, TOCN+CS, and CS were evaluated by pH-potentiometric
titrations. The protein-repellent properties of investigated coatings were
evaluated in situ using a continuous flow of bovine serum albumin (BSA) by
means of
quartz crystal microbalance with dissipation (QCM-D). Antioxidative
activity of TOCN, CS and their amphoteric mixture was determined using the
2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) radical scavenging
assay. Coating with improved protein repellent properties ...was obtained
using TOCN+CS amphoteric mixture, but on the other hand TOCN+CS amphoteric
mixture showed weaker antioxidative properties in comparison to TOCN and
CS.
U ovom radu, filmovi od regenerisane celuloze (RC) su naslojeni
sa TEMPO oksidisanim celuloznim nanofibrilima (TOCN) i hitozanom (CS)
metodom rotirajućeg diska. Bioaktivnost prevlaka postignuta je dodavanjem
hitozana. Hitozan je ili pomešan sa TOCN (TOCN+CS) i naslojen na RC film
tehnikom rotirajućeg diska ili nanesen na RC/TOCN dvoslojni film
propumpavanjem njegovog vodenog rastvora, pH vrednosti 5,5, preko površine
filma.
Naelektrisanje TOCN, TOCN+CS i CS u zavisnosti od pH je okarakterisano
pH-potenciometrijskim titracijama. Protein-odbijajuća svojstva ispitivanih
prevlaka određena su in situ, u kontinualnom toku goveđeg serumskog
albumina (BSA) primenom kvarc-kristal mikrovage sa praćenjem disipacije
(QCM-D). Antioksidativna svojstva TOCN, CS i TOCN+CS amfoterne mešavine su
određena metodom inhibicije radikala
2,2′-azino-bis(3-etil benzotiazolin-6-sulfonske kiseline). Prevlaka sa
poboljšanim protein-odbijajućim svojstvima
je dobijena upotrebom TOCN+CS amfoterne meša...vine, ali sa druge strane
TOCN+CS amfoterna mešavina je pokazala slabija antioksidativna svojstva u
poređenju sa TOCN i CS.
Source:
Proceedings / 55th Meeting of the Serbian Chemical Society, Novi Sad, Serbia, June 8-9, 2018, 2018, 66-76Publisher:
- Beograd : Serbian Chemical Society
Funding / projects:
- Functionalization, characterization and application of cellulose and cellulose derivatives (RS-MESTD-Basic Research (BR or ON)-172029)
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Inovacioni centarTY - CONF AU - Korica, Matea AU - Fras Zemljič, Lidija AU - Bračić, Matej AU - Kargl, Rupert AU - Kostić, Mirjana PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6831 AB - In this work, regenerated cellulose (RC) films was coated with TEMPO oxidized cellulose nanofibrils (TOCN) and chitosan (CS) by means of subsequent spin-coated deposition. The bioactivity of coatings was achieved by addition of chitosan. The chitosan was either mixed with the TOCN (TOCN+CS) and deposited on the RC film by spin-coating or deposited on the RC/TOCN bilayer film by pumping its aqueous solution at pH 5.5 over the surface of the film. The pH dependant charging behaviour of the TOCN, TOCN+CS, and CS were evaluated by pH-potentiometric titrations. The protein-repellent properties of investigated coatings were evaluated in situ using a continuous flow of bovine serum albumin (BSA) by means of quartz crystal microbalance with dissipation (QCM-D). Antioxidative activity of TOCN, CS and their amphoteric mixture was determined using the 2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) radical scavenging assay. Coating with improved protein repellent properties was obtained using TOCN+CS amphoteric mixture, but on the other hand TOCN+CS amphoteric mixture showed weaker antioxidative properties in comparison to TOCN and CS. AB - U ovom radu, filmovi od regenerisane celuloze (RC) su naslojeni sa TEMPO oksidisanim celuloznim nanofibrilima (TOCN) i hitozanom (CS) metodom rotirajućeg diska. Bioaktivnost prevlaka postignuta je dodavanjem hitozana. Hitozan je ili pomešan sa TOCN (TOCN+CS) i naslojen na RC film tehnikom rotirajućeg diska ili nanesen na RC/TOCN dvoslojni film propumpavanjem njegovog vodenog rastvora, pH vrednosti 5,5, preko površine filma. Naelektrisanje TOCN, TOCN+CS i CS u zavisnosti od pH je okarakterisano pH-potenciometrijskim titracijama. Protein-odbijajuća svojstva ispitivanih prevlaka određena su in situ, u kontinualnom toku goveđeg serumskog albumina (BSA) primenom kvarc-kristal mikrovage sa praćenjem disipacije (QCM-D). Antioksidativna svojstva TOCN, CS i TOCN+CS amfoterne mešavine su određena metodom inhibicije radikala 2,2′-azino-bis(3-etil benzotiazolin-6-sulfonske kiseline). Prevlaka sa poboljšanim protein-odbijajućim svojstvima je dobijena upotrebom TOCN+CS amfoterne mešavine, ali sa druge strane TOCN+CS amfoterna mešavina je pokazala slabija antioksidativna svojstva u poređenju sa TOCN i CS. PB - Beograd : Serbian Chemical Society C3 - Proceedings / 55th Meeting of the Serbian Chemical Society, Novi Sad, Serbia, June 8-9, 2018 T1 - Protein-repellent and antioxidative properties of bioactive coatings based on TEMPO oxidized cellulose nanofibrils and chitosan T1 - Protein-odbijajuća i antioksidativna svojstva bioaktivnih prevlaka na bazi TEMPO oksidisanih celuloznih nanofibrila i hitozana EP - 76 SP - 66 UR - https://hdl.handle.net/21.15107/rcub_technorep_6831 ER -
@conference{ author = "Korica, Matea and Fras Zemljič, Lidija and Bračić, Matej and Kargl, Rupert and Kostić, Mirjana", year = "2018", abstract = "In this work, regenerated cellulose (RC) films was coated with TEMPO oxidized cellulose nanofibrils (TOCN) and chitosan (CS) by means of subsequent spin-coated deposition. The bioactivity of coatings was achieved by addition of chitosan. The chitosan was either mixed with the TOCN (TOCN+CS) and deposited on the RC film by spin-coating or deposited on the RC/TOCN bilayer film by pumping its aqueous solution at pH 5.5 over the surface of the film. The pH dependant charging behaviour of the TOCN, TOCN+CS, and CS were evaluated by pH-potentiometric titrations. The protein-repellent properties of investigated coatings were evaluated in situ using a continuous flow of bovine serum albumin (BSA) by means of quartz crystal microbalance with dissipation (QCM-D). Antioxidative activity of TOCN, CS and their amphoteric mixture was determined using the 2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) radical scavenging assay. Coating with improved protein repellent properties was obtained using TOCN+CS amphoteric mixture, but on the other hand TOCN+CS amphoteric mixture showed weaker antioxidative properties in comparison to TOCN and CS., U ovom radu, filmovi od regenerisane celuloze (RC) su naslojeni sa TEMPO oksidisanim celuloznim nanofibrilima (TOCN) i hitozanom (CS) metodom rotirajućeg diska. Bioaktivnost prevlaka postignuta je dodavanjem hitozana. Hitozan je ili pomešan sa TOCN (TOCN+CS) i naslojen na RC film tehnikom rotirajućeg diska ili nanesen na RC/TOCN dvoslojni film propumpavanjem njegovog vodenog rastvora, pH vrednosti 5,5, preko površine filma. Naelektrisanje TOCN, TOCN+CS i CS u zavisnosti od pH je okarakterisano pH-potenciometrijskim titracijama. Protein-odbijajuća svojstva ispitivanih prevlaka određena su in situ, u kontinualnom toku goveđeg serumskog albumina (BSA) primenom kvarc-kristal mikrovage sa praćenjem disipacije (QCM-D). Antioksidativna svojstva TOCN, CS i TOCN+CS amfoterne mešavine su određena metodom inhibicije radikala 2,2′-azino-bis(3-etil benzotiazolin-6-sulfonske kiseline). Prevlaka sa poboljšanim protein-odbijajućim svojstvima je dobijena upotrebom TOCN+CS amfoterne mešavine, ali sa druge strane TOCN+CS amfoterna mešavina je pokazala slabija antioksidativna svojstva u poređenju sa TOCN i CS.", publisher = "Beograd : Serbian Chemical Society", journal = "Proceedings / 55th Meeting of the Serbian Chemical Society, Novi Sad, Serbia, June 8-9, 2018", title = "Protein-repellent and antioxidative properties of bioactive coatings based on TEMPO oxidized cellulose nanofibrils and chitosan, Protein-odbijajuća i antioksidativna svojstva bioaktivnih prevlaka na bazi TEMPO oksidisanih celuloznih nanofibrila i hitozana", pages = "76-66", url = "https://hdl.handle.net/21.15107/rcub_technorep_6831" }
Korica, M., Fras Zemljič, L., Bračić, M., Kargl, R.,& Kostić, M.. (2018). Protein-repellent and antioxidative properties of bioactive coatings based on TEMPO oxidized cellulose nanofibrils and chitosan. in Proceedings / 55th Meeting of the Serbian Chemical Society, Novi Sad, Serbia, June 8-9, 2018 Beograd : Serbian Chemical Society., 66-76. https://hdl.handle.net/21.15107/rcub_technorep_6831
Korica M, Fras Zemljič L, Bračić M, Kargl R, Kostić M. Protein-repellent and antioxidative properties of bioactive coatings based on TEMPO oxidized cellulose nanofibrils and chitosan. in Proceedings / 55th Meeting of the Serbian Chemical Society, Novi Sad, Serbia, June 8-9, 2018. 2018;:66-76. https://hdl.handle.net/21.15107/rcub_technorep_6831 .
Korica, Matea, Fras Zemljič, Lidija, Bračić, Matej, Kargl, Rupert, Kostić, Mirjana, "Protein-repellent and antioxidative properties of bioactive coatings based on TEMPO oxidized cellulose nanofibrils and chitosan" in Proceedings / 55th Meeting of the Serbian Chemical Society, Novi Sad, Serbia, June 8-9, 2018 (2018):66-76, https://hdl.handle.net/21.15107/rcub_technorep_6831 .