Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module
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2023
Authors
Veljković, MilicaSimović, Milica
Banjanac, Katarina
Milivojević, Ana
Ćorović, Marija
Jaćimovski, Darko
Bezbradica, Dejan
Conference object (Published version)
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Scientific breakthroughs in research of human microbiota revealed that significant number of
diseases related to modern food intake and sedentary lifestyles are caused by dysbiosis of human
microbiota composition, hence substantial increase in interest for products helping restore this balance
(such as probiotics and prebiotics) within food, cosmetic and health industry was observed. In recent
times, prebiotics have been recognized as attractive products due to their higher stability to processing
and storage conditions, lower susceptibility to interaction with other compounds in more complex
formulation and higher reproducibility of their physiological effects [1]. Consequential rapid growth of
market demand brought challenges for biochemical industry, such as requirements to increase
production capacity and decrease costs by development of more efficient enzymatic processes and
product separation.
Majority of prebiotic compounds are classified as oligosaccharides, which can... be obtained
either by enzymatic synthesis from compounds of lower degree of polymerization (DP) - for example:
galacto-oligosaccharides (GOS) from lactose and fructo-oligosaccharides (FOS) from sucrose - or by
enzymatic hydrolysis, for example pectic oligosaccharides (POS) from pectin or xylo-oligosaccharides
(XOS) from xylan [2]. In both cases, membrane separation is efficient tool for product purification,
because OS obtained by synthesis can be separated from lower DP compounds using nanofiltrations,
while those obtained by hydrolysis can be separated from compounds of higher DP using ultrafiltration.
Hence, it can be expected that selection of adequate membrane material, pore dimensions and
downstream processing conditions will have important role in development of novel prebiotic
technologies.
In this study nanofiltration membrane module was applied with a purpose of purification of
FOS obtained in enzymatic synthesis using sucrose as substrate. Two strategies were applied:
• purification of reaction mixture obtained after enzymatic synthesis,
• simultaneous enzymatic synthesis and purification in nanofiltration membrane module.
Keywords:
prebiotic / fructosyl transferase / nanofiltration / oligosaccharideSource:
Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain, 2023, 84-85Publisher:
- TECNALIA
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- PrIntPrEnzy - Prebiotics for functional food and bioactive cosmetics produced in intensified enzymatic processes (RS-ScienceFundRS-Ideje-7750109)
- Horizon Europe 2021- 2027 research and innovation programme under grant agreement ID 101060130 (TwinPrebioEnz)
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Institution/Community
Inovacioni centarTY - CONF AU - Veljković, Milica AU - Simović, Milica AU - Banjanac, Katarina AU - Milivojević, Ana AU - Ćorović, Marija AU - Jaćimovski, Darko AU - Bezbradica, Dejan PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6871 AB - Scientific breakthroughs in research of human microbiota revealed that significant number of diseases related to modern food intake and sedentary lifestyles are caused by dysbiosis of human microbiota composition, hence substantial increase in interest for products helping restore this balance (such as probiotics and prebiotics) within food, cosmetic and health industry was observed. In recent times, prebiotics have been recognized as attractive products due to their higher stability to processing and storage conditions, lower susceptibility to interaction with other compounds in more complex formulation and higher reproducibility of their physiological effects [1]. Consequential rapid growth of market demand brought challenges for biochemical industry, such as requirements to increase production capacity and decrease costs by development of more efficient enzymatic processes and product separation. Majority of prebiotic compounds are classified as oligosaccharides, which can be obtained either by enzymatic synthesis from compounds of lower degree of polymerization (DP) - for example: galacto-oligosaccharides (GOS) from lactose and fructo-oligosaccharides (FOS) from sucrose - or by enzymatic hydrolysis, for example pectic oligosaccharides (POS) from pectin or xylo-oligosaccharides (XOS) from xylan [2]. In both cases, membrane separation is efficient tool for product purification, because OS obtained by synthesis can be separated from lower DP compounds using nanofiltrations, while those obtained by hydrolysis can be separated from compounds of higher DP using ultrafiltration. Hence, it can be expected that selection of adequate membrane material, pore dimensions and downstream processing conditions will have important role in development of novel prebiotic technologies. In this study nanofiltration membrane module was applied with a purpose of purification of FOS obtained in enzymatic synthesis using sucrose as substrate. Two strategies were applied: • purification of reaction mixture obtained after enzymatic synthesis, • simultaneous enzymatic synthesis and purification in nanofiltration membrane module. PB - TECNALIA C3 - Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain T1 - Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module EP - 85 EP - SP - 84 UR - https://hdl.handle.net/21.15107/rcub_technorep_6871 ER -
@conference{ author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Milivojević, Ana and Ćorović, Marija and Jaćimovski, Darko and Bezbradica, Dejan", year = "2023", abstract = "Scientific breakthroughs in research of human microbiota revealed that significant number of diseases related to modern food intake and sedentary lifestyles are caused by dysbiosis of human microbiota composition, hence substantial increase in interest for products helping restore this balance (such as probiotics and prebiotics) within food, cosmetic and health industry was observed. In recent times, prebiotics have been recognized as attractive products due to their higher stability to processing and storage conditions, lower susceptibility to interaction with other compounds in more complex formulation and higher reproducibility of their physiological effects [1]. Consequential rapid growth of market demand brought challenges for biochemical industry, such as requirements to increase production capacity and decrease costs by development of more efficient enzymatic processes and product separation. Majority of prebiotic compounds are classified as oligosaccharides, which can be obtained either by enzymatic synthesis from compounds of lower degree of polymerization (DP) - for example: galacto-oligosaccharides (GOS) from lactose and fructo-oligosaccharides (FOS) from sucrose - or by enzymatic hydrolysis, for example pectic oligosaccharides (POS) from pectin or xylo-oligosaccharides (XOS) from xylan [2]. In both cases, membrane separation is efficient tool for product purification, because OS obtained by synthesis can be separated from lower DP compounds using nanofiltrations, while those obtained by hydrolysis can be separated from compounds of higher DP using ultrafiltration. Hence, it can be expected that selection of adequate membrane material, pore dimensions and downstream processing conditions will have important role in development of novel prebiotic technologies. In this study nanofiltration membrane module was applied with a purpose of purification of FOS obtained in enzymatic synthesis using sucrose as substrate. Two strategies were applied: • purification of reaction mixture obtained after enzymatic synthesis, • simultaneous enzymatic synthesis and purification in nanofiltration membrane module.", publisher = "TECNALIA", journal = "Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain", title = "Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module", pages = "85--84", url = "https://hdl.handle.net/21.15107/rcub_technorep_6871" }
Veljković, M., Simović, M., Banjanac, K., Milivojević, A., Ćorović, M., Jaćimovski, D.,& Bezbradica, D.. (2023). Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module. in Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain TECNALIA., 84-85. https://hdl.handle.net/21.15107/rcub_technorep_6871
Veljković M, Simović M, Banjanac K, Milivojević A, Ćorović M, Jaćimovski D, Bezbradica D. Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module. in Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain. 2023;:84-85. https://hdl.handle.net/21.15107/rcub_technorep_6871 .
Veljković, Milica, Simović, Milica, Banjanac, Katarina, Milivojević, Ana, Ćorović, Marija, Jaćimovski, Darko, Bezbradica, Dejan, "Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module" in Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain (2023):84-85, https://hdl.handle.net/21.15107/rcub_technorep_6871 .