Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films
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2023
Authors
Lukić, IvanaMilovanović, Stoja
Krgović, Nemanja
Tadić, Vanja
Tyśkiewicz, Katarzyna
Konkol, Marcin
Conference object (Published version)
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Green tea is considered to be a plant with a variety of health benefits due to its antioxidative, antimicrobial,
anti-inflammatory, and anticancer activity. Besides the most important bioactive constituents, including
polyphenols, especially catechins, and flavonoids, green tea is rich in caffeine, and such complex composition
makes its extract suitable as a component of active food packaging or skin care systems [1,2]. The
technologies commonly proposed to obtain such materials involve some limitations, mainly related to the use
of liquid organic solvents that lead to low penetration of the active compound into the polymeric platform and,
consequently, limited loading efficiencies, non-unique dispersion, and solvent residue in the final material. An
excellent technique for direct loading of natural bioactive compounds from plant materials into polymeric
carriers that can overcome the aforementioned challenges is an integrated process accomplished by merging
supercritical flui...d extraction (SFE) and supercritical solvent impregnation (SSI). Therefore, in this study, the
preparation of two types of polymer films and their functionalization with green tea leaf extract using the SFESSI
process was tested.
Starch/chitosan (SC) films were prepared by mixing the 1% chitosan solution with the 1% starch solution in a
mass ratio of 1:1, while starch/chitosan/β-cyclodextrin (SCCD) films were prepared by addition of 1% β-
cyclodextrin to starch and chitosan to obtain the ratio of 1:1:1. After solvent casting and drying at room
temperature, the films were additionally dried in an oven overnight at 35 °C. Initially, supercritical CO2
(scCO2) extraction was investigated as a method for isolating the extract from green tea at temperatures of 35
and 70 °C and pressure of 30 MPa, with and without the ethanol as a co-solvent. The obtained extraction yields
ranged from 0.91 to 4.22%, being the highest at a higher temperature. The chemical composition of the
obtained extracts was determined, as well as the total phenolic content (TPC), total flavonoid content (TFC),
total chlorophyll, and total carotenoid content. HPLC analysis revealed that caffeine was the major constituent
of all extracts with the amount ranging from 24.1 to 202.9 mg/g. The IC50 values determined using the DPPH
assay showed that the extract obtained at 35 °C with the addition of ethanol exhibited the strongest antioxidant
activity. After determining the SFE conditions that gave the green tea extract with high content of bioactive
compounds, prepared films were impregnated using the integrated SFE-SSI process for 4 h at the same
conditions as scCO2 extraction. The loading of green tea extracts onto films was up to 7.7% showing that lower
temperature (35 °C) and the addition of ethanol had a favourable influence. The presence of the extract on the
surface of impregnated samples was confirmed by FTIR analysis.
Source:
Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023, 2023, 124-Publisher:
- Budapest : University of Technology and Economics
Funding / projects:
- Project No. E!13632 (GREENTECH, EUREKA Programme)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
Institution/Community
Tehnološko-metalurški fakultetTY - CONF AU - Lukić, Ivana AU - Milovanović, Stoja AU - Krgović, Nemanja AU - Tadić, Vanja AU - Tyśkiewicz, Katarzyna AU - Konkol, Marcin PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6907 AB - Green tea is considered to be a plant with a variety of health benefits due to its antioxidative, antimicrobial, anti-inflammatory, and anticancer activity. Besides the most important bioactive constituents, including polyphenols, especially catechins, and flavonoids, green tea is rich in caffeine, and such complex composition makes its extract suitable as a component of active food packaging or skin care systems [1,2]. The technologies commonly proposed to obtain such materials involve some limitations, mainly related to the use of liquid organic solvents that lead to low penetration of the active compound into the polymeric platform and, consequently, limited loading efficiencies, non-unique dispersion, and solvent residue in the final material. An excellent technique for direct loading of natural bioactive compounds from plant materials into polymeric carriers that can overcome the aforementioned challenges is an integrated process accomplished by merging supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). Therefore, in this study, the preparation of two types of polymer films and their functionalization with green tea leaf extract using the SFESSI process was tested. Starch/chitosan (SC) films were prepared by mixing the 1% chitosan solution with the 1% starch solution in a mass ratio of 1:1, while starch/chitosan/β-cyclodextrin (SCCD) films were prepared by addition of 1% β- cyclodextrin to starch and chitosan to obtain the ratio of 1:1:1. After solvent casting and drying at room temperature, the films were additionally dried in an oven overnight at 35 °C. Initially, supercritical CO2 (scCO2) extraction was investigated as a method for isolating the extract from green tea at temperatures of 35 and 70 °C and pressure of 30 MPa, with and without the ethanol as a co-solvent. The obtained extraction yields ranged from 0.91 to 4.22%, being the highest at a higher temperature. The chemical composition of the obtained extracts was determined, as well as the total phenolic content (TPC), total flavonoid content (TFC), total chlorophyll, and total carotenoid content. HPLC analysis revealed that caffeine was the major constituent of all extracts with the amount ranging from 24.1 to 202.9 mg/g. The IC50 values determined using the DPPH assay showed that the extract obtained at 35 °C with the addition of ethanol exhibited the strongest antioxidant activity. After determining the SFE conditions that gave the green tea extract with high content of bioactive compounds, prepared films were impregnated using the integrated SFE-SSI process for 4 h at the same conditions as scCO2 extraction. The loading of green tea extracts onto films was up to 7.7% showing that lower temperature (35 °C) and the addition of ethanol had a favourable influence. The presence of the extract on the surface of impregnated samples was confirmed by FTIR analysis. PB - Budapest : University of Technology and Economics C3 - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 T1 - Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films SP - 124 UR - https://hdl.handle.net/21.15107/rcub_technorep_6907 ER -
@conference{ author = "Lukić, Ivana and Milovanović, Stoja and Krgović, Nemanja and Tadić, Vanja and Tyśkiewicz, Katarzyna and Konkol, Marcin", year = "2023", abstract = "Green tea is considered to be a plant with a variety of health benefits due to its antioxidative, antimicrobial, anti-inflammatory, and anticancer activity. Besides the most important bioactive constituents, including polyphenols, especially catechins, and flavonoids, green tea is rich in caffeine, and such complex composition makes its extract suitable as a component of active food packaging or skin care systems [1,2]. The technologies commonly proposed to obtain such materials involve some limitations, mainly related to the use of liquid organic solvents that lead to low penetration of the active compound into the polymeric platform and, consequently, limited loading efficiencies, non-unique dispersion, and solvent residue in the final material. An excellent technique for direct loading of natural bioactive compounds from plant materials into polymeric carriers that can overcome the aforementioned challenges is an integrated process accomplished by merging supercritical fluid extraction (SFE) and supercritical solvent impregnation (SSI). Therefore, in this study, the preparation of two types of polymer films and their functionalization with green tea leaf extract using the SFESSI process was tested. Starch/chitosan (SC) films were prepared by mixing the 1% chitosan solution with the 1% starch solution in a mass ratio of 1:1, while starch/chitosan/β-cyclodextrin (SCCD) films were prepared by addition of 1% β- cyclodextrin to starch and chitosan to obtain the ratio of 1:1:1. After solvent casting and drying at room temperature, the films were additionally dried in an oven overnight at 35 °C. Initially, supercritical CO2 (scCO2) extraction was investigated as a method for isolating the extract from green tea at temperatures of 35 and 70 °C and pressure of 30 MPa, with and without the ethanol as a co-solvent. The obtained extraction yields ranged from 0.91 to 4.22%, being the highest at a higher temperature. The chemical composition of the obtained extracts was determined, as well as the total phenolic content (TPC), total flavonoid content (TFC), total chlorophyll, and total carotenoid content. HPLC analysis revealed that caffeine was the major constituent of all extracts with the amount ranging from 24.1 to 202.9 mg/g. The IC50 values determined using the DPPH assay showed that the extract obtained at 35 °C with the addition of ethanol exhibited the strongest antioxidant activity. After determining the SFE conditions that gave the green tea extract with high content of bioactive compounds, prepared films were impregnated using the integrated SFE-SSI process for 4 h at the same conditions as scCO2 extraction. The loading of green tea extracts onto films was up to 7.7% showing that lower temperature (35 °C) and the addition of ethanol had a favourable influence. The presence of the extract on the surface of impregnated samples was confirmed by FTIR analysis.", publisher = "Budapest : University of Technology and Economics", journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023", title = "Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films", pages = "124", url = "https://hdl.handle.net/21.15107/rcub_technorep_6907" }
Lukić, I., Milovanović, S., Krgović, N., Tadić, V., Tyśkiewicz, K.,& Konkol, M.. (2023). Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 Budapest : University of Technology and Economics., 124. https://hdl.handle.net/21.15107/rcub_technorep_6907
Lukić I, Milovanović S, Krgović N, Tadić V, Tyśkiewicz K, Konkol M. Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:124. https://hdl.handle.net/21.15107/rcub_technorep_6907 .
Lukić, Ivana, Milovanović, Stoja, Krgović, Nemanja, Tadić, Vanja, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Selection of process conditions for the integrated process of supercritical CO2 extraction from green tea leaves and impregnation onto polymer films" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):124, https://hdl.handle.net/21.15107/rcub_technorep_6907 .