IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS
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2023
Authors
dos Santos, Leandro AlvesBanjanac, Katarina
Veljković, Milica
Simović, Milica
Mateo, Cesar
Bezbradica, Dejan
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A food-grade bacterial xylanase enzyme preparation, ROHALASE® SEP-VISCO,
was immobilized by adsorption onto polyethyleneimine (PEI) functionalized magnetic
nanoparticles (MNPs). This process resulted in nanobiocatalysts with optimal characteristics.
Subsequently, this nanobiocatalyst was employed to produce xylo-oligosaccharides (XOS). In
the past decade, magnetic nanoparticles (MNPs) have gained significant interest, finding
diverse applications in biomedicine, biosensor production, food processing, catalysis,
agriculture, and environmental processes. MNPs have proven highly effective for enzyme
immobilization because of their unique and distinguished properties such as high specific
surface area, biocompatibility, and magnetic characteristics. Magnetic characteristics
facilitate the easy removal of MNPs from reaction mixtures, allowing for their efficient reuse.
The functionalization of MNPs through with the cationic polymer PEI can further augment
their ability for enzyme... attachment. Generally, xylanases hydrolyses β-1,4-glycosidic
linkages present in xylan which is a major component of the hemicellulosic fraction in the
plant cell walls. Furthermore, xylanases can produce XOS composed of xylose units linked
by β-1,4-xylosidic bonds. XOS have stimulatory effects on the selective growth of human
intestinal microbiota and are frequently defined as prebiotics.
In the present work, the xylanase immobilization efficiency on MNPs-PEI is between
100 and 43 % within the wide range of xylanase concentrations (400-2400 mg/g of support).
Free and immobilized xylanase showed maximal catalytic activity at pH 6.0 and 60°C in
reaction with commercial birchwood xylan (concentration of 1 % w/v). The maximum
activity of 1675 IU/g of support was achieved when immobilization was performed at initial
enzyme concentration of 1250 mg/g of support during 2 h. Since this immobilized
preparation exhibited the activity immobilization yield of 80 % and specific activity of 2.1
mg of proteins/g of support, it has been applied in reaction of XOS synthesis. The MNPsPEI-xylanase was found to produce high yield of XOS from birchwood xylan, indicating its
potential for utilization in feed and food formulations.
Keywords:
xylanase / xylo-oligosaccharides / magnetic nanoparticles / immobilization of enzymes / prebioticsSource:
Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023, 34-Publisher:
- Belgrade : University, Faculty of Technology and Metallurgy
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- PrIntPrEnzy - Prebiotics for functional food and bioactive cosmetics produced in intensified enzymatic processes (RS-ScienceFundRS-Ideje-7750109)
- TwinPrebioEnz project which is funded from the Horizon Europe 2021-2027 research and innovation programme under grant agreement ID 101060130
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Inovacioni centarTY - CONF AU - dos Santos, Leandro Alves AU - Banjanac, Katarina AU - Veljković, Milica AU - Simović, Milica AU - Mateo, Cesar AU - Bezbradica, Dejan PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6948 AB - A food-grade bacterial xylanase enzyme preparation, ROHALASE® SEP-VISCO, was immobilized by adsorption onto polyethyleneimine (PEI) functionalized magnetic nanoparticles (MNPs). This process resulted in nanobiocatalysts with optimal characteristics. Subsequently, this nanobiocatalyst was employed to produce xylo-oligosaccharides (XOS). In the past decade, magnetic nanoparticles (MNPs) have gained significant interest, finding diverse applications in biomedicine, biosensor production, food processing, catalysis, agriculture, and environmental processes. MNPs have proven highly effective for enzyme immobilization because of their unique and distinguished properties such as high specific surface area, biocompatibility, and magnetic characteristics. Magnetic characteristics facilitate the easy removal of MNPs from reaction mixtures, allowing for their efficient reuse. The functionalization of MNPs through with the cationic polymer PEI can further augment their ability for enzyme attachment. Generally, xylanases hydrolyses β-1,4-glycosidic linkages present in xylan which is a major component of the hemicellulosic fraction in the plant cell walls. Furthermore, xylanases can produce XOS composed of xylose units linked by β-1,4-xylosidic bonds. XOS have stimulatory effects on the selective growth of human intestinal microbiota and are frequently defined as prebiotics. In the present work, the xylanase immobilization efficiency on MNPs-PEI is between 100 and 43 % within the wide range of xylanase concentrations (400-2400 mg/g of support). Free and immobilized xylanase showed maximal catalytic activity at pH 6.0 and 60°C in reaction with commercial birchwood xylan (concentration of 1 % w/v). The maximum activity of 1675 IU/g of support was achieved when immobilization was performed at initial enzyme concentration of 1250 mg/g of support during 2 h. Since this immobilized preparation exhibited the activity immobilization yield of 80 % and specific activity of 2.1 mg of proteins/g of support, it has been applied in reaction of XOS synthesis. The MNPsPEI-xylanase was found to produce high yield of XOS from birchwood xylan, indicating its potential for utilization in feed and food formulations. PB - Belgrade : University, Faculty of Technology and Metallurgy C3 - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade T1 - IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS SP - 34 UR - https://hdl.handle.net/21.15107/rcub_technorep_6948 ER -
@conference{ author = "dos Santos, Leandro Alves and Banjanac, Katarina and Veljković, Milica and Simović, Milica and Mateo, Cesar and Bezbradica, Dejan", year = "2023", abstract = "A food-grade bacterial xylanase enzyme preparation, ROHALASE® SEP-VISCO, was immobilized by adsorption onto polyethyleneimine (PEI) functionalized magnetic nanoparticles (MNPs). This process resulted in nanobiocatalysts with optimal characteristics. Subsequently, this nanobiocatalyst was employed to produce xylo-oligosaccharides (XOS). In the past decade, magnetic nanoparticles (MNPs) have gained significant interest, finding diverse applications in biomedicine, biosensor production, food processing, catalysis, agriculture, and environmental processes. MNPs have proven highly effective for enzyme immobilization because of their unique and distinguished properties such as high specific surface area, biocompatibility, and magnetic characteristics. Magnetic characteristics facilitate the easy removal of MNPs from reaction mixtures, allowing for their efficient reuse. The functionalization of MNPs through with the cationic polymer PEI can further augment their ability for enzyme attachment. Generally, xylanases hydrolyses β-1,4-glycosidic linkages present in xylan which is a major component of the hemicellulosic fraction in the plant cell walls. Furthermore, xylanases can produce XOS composed of xylose units linked by β-1,4-xylosidic bonds. XOS have stimulatory effects on the selective growth of human intestinal microbiota and are frequently defined as prebiotics. In the present work, the xylanase immobilization efficiency on MNPs-PEI is between 100 and 43 % within the wide range of xylanase concentrations (400-2400 mg/g of support). Free and immobilized xylanase showed maximal catalytic activity at pH 6.0 and 60°C in reaction with commercial birchwood xylan (concentration of 1 % w/v). The maximum activity of 1675 IU/g of support was achieved when immobilization was performed at initial enzyme concentration of 1250 mg/g of support during 2 h. Since this immobilized preparation exhibited the activity immobilization yield of 80 % and specific activity of 2.1 mg of proteins/g of support, it has been applied in reaction of XOS synthesis. The MNPsPEI-xylanase was found to produce high yield of XOS from birchwood xylan, indicating its potential for utilization in feed and food formulations.", publisher = "Belgrade : University, Faculty of Technology and Metallurgy", journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade", title = "IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS", pages = "34", url = "https://hdl.handle.net/21.15107/rcub_technorep_6948" }
dos Santos, L. A., Banjanac, K., Veljković, M., Simović, M., Mateo, C.,& Bezbradica, D.. (2023). IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade Belgrade : University, Faculty of Technology and Metallurgy., 34. https://hdl.handle.net/21.15107/rcub_technorep_6948
dos Santos LA, Banjanac K, Veljković M, Simović M, Mateo C, Bezbradica D. IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:34. https://hdl.handle.net/21.15107/rcub_technorep_6948 .
dos Santos, Leandro Alves, Banjanac, Katarina, Veljković, Milica, Simović, Milica, Mateo, Cesar, Bezbradica, Dejan, "IMMOBILIZATION OF XYLANASE ON MAGNETIC NANOPARTICLES MODIFIED WITH POLYETHYLENEIMINE AND ITS APPLICATION IN XYLO-OLIGOSACCHARIDES SYNTHESIS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):34, https://hdl.handle.net/21.15107/rcub_technorep_6948 .