NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME
Samo za registrovane korisnike
2023
Autori
Ortega-Nieto, ClaraLosada-Gracia, Noelia
Vukoičić, Ana
Milivojević, Ana
Bezbradica, Dejan
Palomo, Jose M.
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In recent years, the synthesis of novel artificial metalloenzymes by the combination of
metal or complex organometallic systems and enzymes is rapidly growing field of research in
catalytic applications. In this study, new artificial metalloenzymes have been designed and
developed based on the combination of an enzyme and different metal salts. For this purpose,
novel synthesis method has been used, where the enzyme, Lipase B from Candida antarctica
(CALB), acts as an inducer of metal nanoparticles of copper and silver. Obtained biohybrids
show improved stability properties compared to natural enzymes. Moreover, the synergetic
effect between copper and silver has been evaluated, looking for an improvement in the
catalytic efficiency of an only copper-metalloenzyme previously developed.
The copper-silver-CALB metalloenzymes have been characterized by X-Ray
diffraction analysis, mass spectrometry, and SEM and TEM as microscopies techniques. After
that, their catalytic efficien...cy was tested in different reactions, in particular in the phloridzin
oligomerization as laccase-like activity.
Ključne reči:
metalloenzymes / copper / silver / phloridzin / lacasse-like activityIzvor:
Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023, 39-Izdavač:
- Belgrade : University, Faculty of Technology and Metallurgy
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-MESTD-inst-2020-200135)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200287 (Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (RS-MESTD-inst-2020-200287)
- Horizon Europe 2021-2027 research and innovation programme under grant agreement ID 101060130 (TwinPrebioEnz)
Kolekcije
Institucija/grupa
Inovacioni centarTY - CONF AU - Ortega-Nieto, Clara AU - Losada-Gracia, Noelia AU - Vukoičić, Ana AU - Milivojević, Ana AU - Bezbradica, Dejan AU - Palomo, Jose M. PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6959 AB - In recent years, the synthesis of novel artificial metalloenzymes by the combination of metal or complex organometallic systems and enzymes is rapidly growing field of research in catalytic applications. In this study, new artificial metalloenzymes have been designed and developed based on the combination of an enzyme and different metal salts. For this purpose, novel synthesis method has been used, where the enzyme, Lipase B from Candida antarctica (CALB), acts as an inducer of metal nanoparticles of copper and silver. Obtained biohybrids show improved stability properties compared to natural enzymes. Moreover, the synergetic effect between copper and silver has been evaluated, looking for an improvement in the catalytic efficiency of an only copper-metalloenzyme previously developed. The copper-silver-CALB metalloenzymes have been characterized by X-Ray diffraction analysis, mass spectrometry, and SEM and TEM as microscopies techniques. After that, their catalytic efficiency was tested in different reactions, in particular in the phloridzin oligomerization as laccase-like activity. PB - Belgrade : University, Faculty of Technology and Metallurgy C3 - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade T1 - NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME SP - 39 UR - https://hdl.handle.net/21.15107/rcub_technorep_6959 ER -
@conference{ author = "Ortega-Nieto, Clara and Losada-Gracia, Noelia and Vukoičić, Ana and Milivojević, Ana and Bezbradica, Dejan and Palomo, Jose M.", year = "2023", abstract = "In recent years, the synthesis of novel artificial metalloenzymes by the combination of metal or complex organometallic systems and enzymes is rapidly growing field of research in catalytic applications. In this study, new artificial metalloenzymes have been designed and developed based on the combination of an enzyme and different metal salts. For this purpose, novel synthesis method has been used, where the enzyme, Lipase B from Candida antarctica (CALB), acts as an inducer of metal nanoparticles of copper and silver. Obtained biohybrids show improved stability properties compared to natural enzymes. Moreover, the synergetic effect between copper and silver has been evaluated, looking for an improvement in the catalytic efficiency of an only copper-metalloenzyme previously developed. The copper-silver-CALB metalloenzymes have been characterized by X-Ray diffraction analysis, mass spectrometry, and SEM and TEM as microscopies techniques. After that, their catalytic efficiency was tested in different reactions, in particular in the phloridzin oligomerization as laccase-like activity.", publisher = "Belgrade : University, Faculty of Technology and Metallurgy", journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade", title = "NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME", pages = "39", url = "https://hdl.handle.net/21.15107/rcub_technorep_6959" }
Ortega-Nieto, C., Losada-Gracia, N., Vukoičić, A., Milivojević, A., Bezbradica, D.,& Palomo, J. M.. (2023). NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade Belgrade : University, Faculty of Technology and Metallurgy., 39. https://hdl.handle.net/21.15107/rcub_technorep_6959
Ortega-Nieto C, Losada-Gracia N, Vukoičić A, Milivojević A, Bezbradica D, Palomo JM. NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:39. https://hdl.handle.net/21.15107/rcub_technorep_6959 .
Ortega-Nieto, Clara, Losada-Gracia, Noelia, Vukoičić, Ana, Milivojević, Ana, Bezbradica, Dejan, Palomo, Jose M., "NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):39, https://hdl.handle.net/21.15107/rcub_technorep_6959 .