Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications
Authors
Jaćimović, NevenaĐošić, Marija
Janković, Ana
Grujić, Svetlana
Matić Bujagić, Ivana
Stojanović, Jovica
Vukašinović-Sekulić, Maja
Kojić, Vesna
Mišković-Stanković, Vesna
Article (Published version)
Metadata
Show full item recordAbstract
Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) after immersion in simulated body fluid (SBF) at 37 °C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect” with ~ 30 % of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureus TL and Escherich-ia coli ATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion t...est (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.
Keywords:
electrophoretic deposition / Chitosan / poly(vinyl alcohol) / gentamicin release / antibacterialSource:
Macedonian Journal of Chemistry and Chemical Engineering, 2023, 42, 2, 249-262Publisher:
- Society of Chemists and Technologists of Macedonia
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200023 (Institute of Technology of Nuclear and Other Mineral Row Materials - ITNMS, Belgrade) (RS-MESTD-inst-2020-200023)
- Ministry of Science, Technological Development and Innova-tion, Republic of Serbia (Contract No. 337-00-110/2023-05/13)
- European Union’s Horizon 2020 research and innovation program under grant agreement No. 952033
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Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Jaćimović, Nevena AU - Đošić, Marija AU - Janković, Ana AU - Grujić, Svetlana AU - Matić Bujagić, Ivana AU - Stojanović, Jovica AU - Vukašinović-Sekulić, Maja AU - Kojić, Vesna AU - Mišković-Stanković, Vesna PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7260 AB - Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) after immersion in simulated body fluid (SBF) at 37 °C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect” with ~ 30 % of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureus TL and Escherich-ia coli ATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes. PB - Society of Chemists and Technologists of Macedonia T2 - Macedonian Journal of Chemistry and Chemical Engineering T1 - Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications EP - 262 IS - 2 SP - 249 VL - 42 DO - 10.20450/mjcce.2023.2775 ER -
@article{ author = "Jaćimović, Nevena and Đošić, Marija and Janković, Ana and Grujić, Svetlana and Matić Bujagić, Ivana and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna", year = "2023", abstract = "Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) after immersion in simulated body fluid (SBF) at 37 °C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect” with ~ 30 % of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureus TL and Escherich-ia coli ATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.", publisher = "Society of Chemists and Technologists of Macedonia", journal = "Macedonian Journal of Chemistry and Chemical Engineering", title = "Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications", pages = "262-249", number = "2", volume = "42", doi = "10.20450/mjcce.2023.2775" }
Jaćimović, N., Đošić, M., Janković, A., Grujić, S., Matić Bujagić, I., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering Society of Chemists and Technologists of Macedonia., 42(2), 249-262. https://doi.org/10.20450/mjcce.2023.2775
Jaćimović N, Đošić M, Janković A, Grujić S, Matić Bujagić I, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering. 2023;42(2):249-262. doi:10.20450/mjcce.2023.2775 .
Jaćimović, Nevena, Đošić, Marija, Janković, Ana, Grujić, Svetlana, Matić Bujagić, Ivana, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications" in Macedonian Journal of Chemistry and Chemical Engineering, 42, no. 2 (2023):249-262, https://doi.org/10.20450/mjcce.2023.2775 . .