Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients
Аутори
Đukić, TamaraDrvenica, Ivana
Kovačić, Marijana
Milanović, Slađan
Majerič, Dragana
Šefik-Bukilica, Mirjana
Miletić, Maja
Bugarski, Branko
Ilić, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin lev...el. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.
Кључне речи:
autoantibodies against citrullinated peptides / circulating immune complexes / dynamic light scattering / immunoglobulin content / rheumatoid factorИзвор:
International Journal of Molecular Sciences, 2024, 25, 6, 3138-Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200015 (Универзитет у Београду, Институт за медицинска истраживања) (RS-MESTD-inst-2020-200015)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
Колекције
Институција/група
Inovacioni centarTY - JOUR AU - Đukić, Tamara AU - Drvenica, Ivana AU - Kovačić, Marijana AU - Milanović, Slađan AU - Majerič, Dragana AU - Šefik-Bukilica, Mirjana AU - Miletić, Maja AU - Bugarski, Branko AU - Ilić, Vesna PY - 2024 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7411 AB - The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation. PB - MDPI T2 - International Journal of Molecular Sciences T1 - Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients IS - 6 SP - 3138 VL - 25 DO - 10.3390/ijms25063138 ER -
@article{ author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Milanović, Slađan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Miletić, Maja and Bugarski, Branko and Ilić, Vesna", year = "2024", abstract = "The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.", publisher = "MDPI", journal = "International Journal of Molecular Sciences", title = "Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients", number = "6", pages = "3138", volume = "25", doi = "10.3390/ijms25063138" }
Đukić, T., Drvenica, I., Kovačić, M., Milanović, S., Majerič, D., Šefik-Bukilica, M., Miletić, M., Bugarski, B.,& Ilić, V.. (2024). Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients. in International Journal of Molecular Sciences MDPI., 25(6), 3138. https://doi.org/10.3390/ijms25063138
Đukić T, Drvenica I, Kovačić M, Milanović S, Majerič D, Šefik-Bukilica M, Miletić M, Bugarski B, Ilić V. Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients. in International Journal of Molecular Sciences. 2024;25(6):3138. doi:10.3390/ijms25063138 .
Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Milanović, Slađan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Miletić, Maja, Bugarski, Branko, Ilić, Vesna, "Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients" in International Journal of Molecular Sciences, 25, no. 6 (2024):3138, https://doi.org/10.3390/ijms25063138 . .