Bioethanol from corn meal hydrolyzates
Proizvodnja bioetanola na hidrolizovanom kukuruznom brašnu
Authors
Nikolić, SvetlanaRakin, Marica
Vukašinović-Sekulić, Maja
Šiler-Marinković, Slavica
Mojović, Ljiljana
Article (Published version)
Metadata
Show full item recordAbstract
The two-step enzymatic hydrolysis of corn meal by commercially available α-amylase and amyloglycosidase and the subsequent or simultaneous ethanol fermentation of the hydrolyzates by Saccharomyces cerevisiae yeast were studied. The conditions of starch hydrolysis, such as substrate and enzyme concentration and the time required for enzymatic action, were optimized taking into account both the effects of hydrolysis and ethanol fermentation. The corn meal hydrolyzates obtained were good substrates for ethanol fermentation by Saccharomyces cerevisiae. A yield of ethanol of more than 80% of the theoretical one was achieved with a satisfactory product to substrate yield Yp/s (g/g) and good ethanol volumetric productivity P (g/lh). No shortage of fermentable sugars was observed during simultaneous hydrolysis and fermentation. Savings in time and energy could be realized by such a process.
U radu je ispitivan postupak dvojno enzimske hidrolize kukuruznog brašna pomoću komercijalnih enzima α-amilaze i amiloglukozidaze i naknadna ili uporedna fermentacija hidrolizata pomoću kvasca Saccharomyces cerevisiae. Uslovi hidrolize kao što su koncentracija supstrata i enzima i vreme dejstva pojedinih enzima su optimizovani sa aspekta oba procesa (i fermentacije i hidrolize). Dobijeni hidrolizati kukuruznog brašna su se pokazali kao pogodni supstrati za etanolnu fermentaciju pomoću kvasca Saccharomyces cerevisiae pri čemu je ostvaren prinos etanola od više od 80% od teorijskog prinosa i sa zadovoljavajućim vrednostima prinosa produkta po substratu Yp/s (g/g) i volumetrijske produktivnoosti P (g/lh). U toku postupka sa uporednim odvijanjem hidrolize i fermentacije nije primećen nedostatak fermentabilnih šećera. Osim toga ovim postupkom moguće je skratiti ukupno vreme procesa i ostvariti određene energetske uštede.
Keywords:
enzyme hydrolysis / starch / corn / ethanol fermentation / enzimska hidroliza / skrob / kukuruz / alkoholna fermentacijaSource:
Chemical Industry & Chemical Engineering Quarterly, 2005, 11, 4, 189-194Publisher:
- Association of the Chemical Engineers of Serbia
Funding / projects:
- Projekat Ministarstva nauke Republike Srbije, br. TD 7042B
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Nikolić, Svetlana AU - Rakin, Marica AU - Vukašinović-Sekulić, Maja AU - Šiler-Marinković, Slavica AU - Mojović, Ljiljana PY - 2005 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/755 AB - The two-step enzymatic hydrolysis of corn meal by commercially available α-amylase and amyloglycosidase and the subsequent or simultaneous ethanol fermentation of the hydrolyzates by Saccharomyces cerevisiae yeast were studied. The conditions of starch hydrolysis, such as substrate and enzyme concentration and the time required for enzymatic action, were optimized taking into account both the effects of hydrolysis and ethanol fermentation. The corn meal hydrolyzates obtained were good substrates for ethanol fermentation by Saccharomyces cerevisiae. A yield of ethanol of more than 80% of the theoretical one was achieved with a satisfactory product to substrate yield Yp/s (g/g) and good ethanol volumetric productivity P (g/lh). No shortage of fermentable sugars was observed during simultaneous hydrolysis and fermentation. Savings in time and energy could be realized by such a process. AB - U radu je ispitivan postupak dvojno enzimske hidrolize kukuruznog brašna pomoću komercijalnih enzima α-amilaze i amiloglukozidaze i naknadna ili uporedna fermentacija hidrolizata pomoću kvasca Saccharomyces cerevisiae. Uslovi hidrolize kao što su koncentracija supstrata i enzima i vreme dejstva pojedinih enzima su optimizovani sa aspekta oba procesa (i fermentacije i hidrolize). Dobijeni hidrolizati kukuruznog brašna su se pokazali kao pogodni supstrati za etanolnu fermentaciju pomoću kvasca Saccharomyces cerevisiae pri čemu je ostvaren prinos etanola od više od 80% od teorijskog prinosa i sa zadovoljavajućim vrednostima prinosa produkta po substratu Yp/s (g/g) i volumetrijske produktivnoosti P (g/lh). U toku postupka sa uporednim odvijanjem hidrolize i fermentacije nije primećen nedostatak fermentabilnih šećera. Osim toga ovim postupkom moguće je skratiti ukupno vreme procesa i ostvariti određene energetske uštede. PB - Association of the Chemical Engineers of Serbia T2 - Chemical Industry & Chemical Engineering Quarterly T1 - Bioethanol from corn meal hydrolyzates T1 - Proizvodnja bioetanola na hidrolizovanom kukuruznom brašnu EP - 194 IS - 4 SP - 189 VL - 11 DO - 10.2298/CICEQ0504189N ER -
@article{ author = "Nikolić, Svetlana and Rakin, Marica and Vukašinović-Sekulić, Maja and Šiler-Marinković, Slavica and Mojović, Ljiljana", year = "2005", abstract = "The two-step enzymatic hydrolysis of corn meal by commercially available α-amylase and amyloglycosidase and the subsequent or simultaneous ethanol fermentation of the hydrolyzates by Saccharomyces cerevisiae yeast were studied. The conditions of starch hydrolysis, such as substrate and enzyme concentration and the time required for enzymatic action, were optimized taking into account both the effects of hydrolysis and ethanol fermentation. The corn meal hydrolyzates obtained were good substrates for ethanol fermentation by Saccharomyces cerevisiae. A yield of ethanol of more than 80% of the theoretical one was achieved with a satisfactory product to substrate yield Yp/s (g/g) and good ethanol volumetric productivity P (g/lh). No shortage of fermentable sugars was observed during simultaneous hydrolysis and fermentation. Savings in time and energy could be realized by such a process., U radu je ispitivan postupak dvojno enzimske hidrolize kukuruznog brašna pomoću komercijalnih enzima α-amilaze i amiloglukozidaze i naknadna ili uporedna fermentacija hidrolizata pomoću kvasca Saccharomyces cerevisiae. Uslovi hidrolize kao što su koncentracija supstrata i enzima i vreme dejstva pojedinih enzima su optimizovani sa aspekta oba procesa (i fermentacije i hidrolize). Dobijeni hidrolizati kukuruznog brašna su se pokazali kao pogodni supstrati za etanolnu fermentaciju pomoću kvasca Saccharomyces cerevisiae pri čemu je ostvaren prinos etanola od više od 80% od teorijskog prinosa i sa zadovoljavajućim vrednostima prinosa produkta po substratu Yp/s (g/g) i volumetrijske produktivnoosti P (g/lh). U toku postupka sa uporednim odvijanjem hidrolize i fermentacije nije primećen nedostatak fermentabilnih šećera. Osim toga ovim postupkom moguće je skratiti ukupno vreme procesa i ostvariti određene energetske uštede.", publisher = "Association of the Chemical Engineers of Serbia", journal = "Chemical Industry & Chemical Engineering Quarterly", title = "Bioethanol from corn meal hydrolyzates, Proizvodnja bioetanola na hidrolizovanom kukuruznom brašnu", pages = "194-189", number = "4", volume = "11", doi = "10.2298/CICEQ0504189N" }
Nikolić, S., Rakin, M., Vukašinović-Sekulić, M., Šiler-Marinković, S.,& Mojović, L.. (2005). Bioethanol from corn meal hydrolyzates. in Chemical Industry & Chemical Engineering Quarterly Association of the Chemical Engineers of Serbia., 11(4), 189-194. https://doi.org/10.2298/CICEQ0504189N
Nikolić S, Rakin M, Vukašinović-Sekulić M, Šiler-Marinković S, Mojović L. Bioethanol from corn meal hydrolyzates. in Chemical Industry & Chemical Engineering Quarterly. 2005;11(4):189-194. doi:10.2298/CICEQ0504189N .
Nikolić, Svetlana, Rakin, Marica, Vukašinović-Sekulić, Maja, Šiler-Marinković, Slavica, Mojović, Ljiljana, "Bioethanol from corn meal hydrolyzates" in Chemical Industry & Chemical Engineering Quarterly, 11, no. 4 (2005):189-194, https://doi.org/10.2298/CICEQ0504189N . .