Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs
Apstrakt
The limited ability of cardiac muscle to regenerate after injury and the small number of organs available for transplantation motivate studies aimed at curative treatment options. Tissue engineering based on the integrated use of cells on biomaterial scaffolds in bioreactors may offer cardiac grafts suitable for surgical attachment to the myocardium or for basic research. In one of the current approaches, neonatal rat cardiomyocytes are combined with collagen sponges, gels or polyglycolic acid scaffolds (PGA). Cultivations performed in dishes, static or mixed flasks or rotating bioreactors yield constructs with a thin (100-200 mu m) peripheral layer of tissue expressing markers of cardiac differentiation and able to propagate electrical signals. The non-uniform cell distribution is a result of oxygen diffusional limitations within the constructs. Cultivations with perfusion of culture medium through the construct enhance the convective-diffusive oxygen supply and yield 1-2mm thick cons...tructs with physiologically high and spatially uniform distribution of viable cells expressing cardiac markers. We review here a series of studies we conducted using cells seeded on three-dimensional scaffolds and cultured in several different bioreactors, to demonstrate that the bioreactor flow environment can have substantial effects on structural and functional properties of cardiac constructs.
Ključne reči:
cardiac tissue / tissue constructs / biomaterial scaffold / bioreactorIzvor:
Journal of Chemical Technology and Biotechnology, 2006, 81, 4, 485-490Izdavač:
- Wiley, Hoboken
DOI: 10.1002/jctb.1467
ISSN: 0268-2575
WoS: 000236946500002
Scopus: 2-s2.0-33646195870
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Vunjak-Novaković, Gordana AU - Radisić, M AU - Obradović, Bojana PY - 2006 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/920 AB - The limited ability of cardiac muscle to regenerate after injury and the small number of organs available for transplantation motivate studies aimed at curative treatment options. Tissue engineering based on the integrated use of cells on biomaterial scaffolds in bioreactors may offer cardiac grafts suitable for surgical attachment to the myocardium or for basic research. In one of the current approaches, neonatal rat cardiomyocytes are combined with collagen sponges, gels or polyglycolic acid scaffolds (PGA). Cultivations performed in dishes, static or mixed flasks or rotating bioreactors yield constructs with a thin (100-200 mu m) peripheral layer of tissue expressing markers of cardiac differentiation and able to propagate electrical signals. The non-uniform cell distribution is a result of oxygen diffusional limitations within the constructs. Cultivations with perfusion of culture medium through the construct enhance the convective-diffusive oxygen supply and yield 1-2mm thick constructs with physiologically high and spatially uniform distribution of viable cells expressing cardiac markers. We review here a series of studies we conducted using cells seeded on three-dimensional scaffolds and cultured in several different bioreactors, to demonstrate that the bioreactor flow environment can have substantial effects on structural and functional properties of cardiac constructs. PB - Wiley, Hoboken T2 - Journal of Chemical Technology and Biotechnology T1 - Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs EP - 490 IS - 4 SP - 485 VL - 81 DO - 10.1002/jctb.1467 ER -
@article{ author = "Vunjak-Novaković, Gordana and Radisić, M and Obradović, Bojana", year = "2006", abstract = "The limited ability of cardiac muscle to regenerate after injury and the small number of organs available for transplantation motivate studies aimed at curative treatment options. Tissue engineering based on the integrated use of cells on biomaterial scaffolds in bioreactors may offer cardiac grafts suitable for surgical attachment to the myocardium or for basic research. In one of the current approaches, neonatal rat cardiomyocytes are combined with collagen sponges, gels or polyglycolic acid scaffolds (PGA). Cultivations performed in dishes, static or mixed flasks or rotating bioreactors yield constructs with a thin (100-200 mu m) peripheral layer of tissue expressing markers of cardiac differentiation and able to propagate electrical signals. The non-uniform cell distribution is a result of oxygen diffusional limitations within the constructs. Cultivations with perfusion of culture medium through the construct enhance the convective-diffusive oxygen supply and yield 1-2mm thick constructs with physiologically high and spatially uniform distribution of viable cells expressing cardiac markers. We review here a series of studies we conducted using cells seeded on three-dimensional scaffolds and cultured in several different bioreactors, to demonstrate that the bioreactor flow environment can have substantial effects on structural and functional properties of cardiac constructs.", publisher = "Wiley, Hoboken", journal = "Journal of Chemical Technology and Biotechnology", title = "Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs", pages = "490-485", number = "4", volume = "81", doi = "10.1002/jctb.1467" }
Vunjak-Novaković, G., Radisić, M.,& Obradović, B.. (2006). Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs. in Journal of Chemical Technology and Biotechnology Wiley, Hoboken., 81(4), 485-490. https://doi.org/10.1002/jctb.1467
Vunjak-Novaković G, Radisić M, Obradović B. Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs. in Journal of Chemical Technology and Biotechnology. 2006;81(4):485-490. doi:10.1002/jctb.1467 .
Vunjak-Novaković, Gordana, Radisić, M, Obradović, Bojana, "Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs" in Journal of Chemical Technology and Biotechnology, 81, no. 4 (2006):485-490, https://doi.org/10.1002/jctb.1467 . .