@article{
author = "Antov, Mirjana and Ivetić, Darjana Z. and Knežević-Jugović, Zorica",
year = "2016",
abstract = "Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w(-1)) polyethylene glycol 4000/15% (w w(-1)) KH2PO4/70% (w w(-1)) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Separation Science and Technology",
title = "Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction",
pages = "628-622",
number = "4",
volume = "51",
doi = "10.1080/01496395.2015.1117106"
}