Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies
Нема приказа
Аутори
Obradović, BojanaOsmokrović, Andrea
Bugarski, Branko
Bugarski, Diana
Vunjak-Novaković, Gordana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.
Кључне речи:
alginate / bioreactor / cartilage tissue engineering / cell immobilizationИзвор:
Materials Science Forum, 2007, 555, 417-422Издавач:
- 8th Conference of the Yugoslav Materials Research Society
Финансирање / пројекти:
- Интеракција имобилисаних ћелија, ткива и биолошки активних молекула у биореакторским системима (RS-MESTD-MPN2006-2010-142075)
DOI: 10.4028/0-87849-441-3.417
ISSN: 0255-5476
PubMed:
WoS: 000249653700067
Scopus: 2-s2.0-38349010697
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Obradović, Bojana AU - Osmokrović, Andrea AU - Bugarski, Branko AU - Bugarski, Diana AU - Vunjak-Novaković, Gordana PY - 2007 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1018 AB - Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering. PB - 8th Conference of the Yugoslav Materials Research Society T2 - Materials Science Forum T1 - Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies EP - 422 SP - 417 VL - 555 DO - 10.4028/0-87849-441-3.417 ER -
@article{ author = "Obradović, Bojana and Osmokrović, Andrea and Bugarski, Branko and Bugarski, Diana and Vunjak-Novaković, Gordana", year = "2007", abstract = "Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.", publisher = "8th Conference of the Yugoslav Materials Research Society", journal = "Materials Science Forum", title = "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies", pages = "422-417", volume = "555", doi = "10.4028/0-87849-441-3.417" }
Obradović, B., Osmokrović, A., Bugarski, B., Bugarski, D.,& Vunjak-Novaković, G.. (2007). Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Materials Science Forum 8th Conference of the Yugoslav Materials Research Society., 555, 417-422. https://doi.org/10.4028/0-87849-441-3.417
Obradović B, Osmokrović A, Bugarski B, Bugarski D, Vunjak-Novaković G. Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Materials Science Forum. 2007;555:417-422. doi:10.4028/0-87849-441-3.417 .
Obradović, Bojana, Osmokrović, Andrea, Bugarski, Branko, Bugarski, Diana, Vunjak-Novaković, Gordana, "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies" in Materials Science Forum, 555 (2007):417-422, https://doi.org/10.4028/0-87849-441-3.417 . .