Приказ основних података о документу
Preparation and characterization of penicillin acylase immobilized on sepabeads EC-EP carrier
dc.creator | Žuža, Milena | |
dc.creator | Šiler-Marinković, Slavica | |
dc.creator | Knežević, Zorica | |
dc.date.accessioned | 2021-03-10T10:41:28Z | |
dc.date.available | 2021-03-10T10:41:28Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 1451-9372 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1061 | |
dc.description.abstract | This paper reports the covalent immobilization of penicillin G acylase from E. coli on Sepabeads EC-EP, an epoxy-activated polymethacrylic carrier, and describes the properties of the immobilized enzyme. Due to its versatility to mediate hydrolysis of penicillins and semi-synthetic B-lactam antibiotics synthesis reactions, the selected enzyme belongs to a class of biocatalysts of great industrial interest. The immobilized enzyme was characterized in its pH and thermal stability and reaction kinetics. The immobilization of penicillin acylase resulted in a slightly different pH activity profile and temperature optima, indicating that the immobilization by this method imparted the structural and conformational stability to this enzyme. The immobilized enzyme also retained a high catalytic activity and showed the increased thermal stability compared with a free enzyme. By comparison of decimal reduction time values obtained at 50°C, it can be concluded that the immobilized enzyme was approximately 5-fold more stable than a free enzyme. The immobilization procedure developed is quite simple and easily reproduced, and provides a promising solution for the application of penicillin acylase for the purpose of 6-aminopenicillanic acid production. | en |
dc.publisher | Association of the Chemical Engineers of Serbia | |
dc.relation | Projekat Ministarstva nauke Republike Srbije, br. BTH1008 | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Chemical Industry & Chemical Engineering Quarterly | |
dc.subject | penicillin G acylase | en |
dc.subject | covalent immobilization | en |
dc.subject | epoxy-activated polymethacrylic carrier | en |
dc.subject | sepabeads EC-EP | en |
dc.subject | thermal stability | en |
dc.title | Preparation and characterization of penicillin acylase immobilized on sepabeads EC-EP carrier | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.epage | 210 | |
dc.citation.issue | 4 | |
dc.citation.other | 13(4): 205-210 | |
dc.citation.spage | 205 | |
dc.citation.volume | 13 | |
dc.identifier.doi | 10.2298/CICEQ0704205Z | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/8572/1451-93720704205Z.pdf | |
dc.identifier.scopus | 2-s2.0-60249094573 | |
dc.type.version | publishedVersion |