Приказ основних података о документу

dc.creatorŽuža, Milena
dc.creatorŠiler-Marinković, Slavica
dc.creatorKnežević, Zorica
dc.date.accessioned2021-03-10T10:41:28Z
dc.date.available2021-03-10T10:41:28Z
dc.date.issued2007
dc.identifier.issn1451-9372
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/1061
dc.description.abstractThis paper reports the covalent immobilization of penicillin G acylase from E. coli on Sepabeads EC-EP, an epoxy-activated polymethacrylic carrier, and describes the properties of the immobilized enzyme. Due to its versatility to mediate hydrolysis of penicillins and semi-synthetic B-lactam antibiotics synthesis reactions, the selected enzyme belongs to a class of biocatalysts of great industrial interest. The immobilized enzyme was characterized in its pH and thermal stability and reaction kinetics. The immobilization of penicillin acylase resulted in a slightly different pH activity profile and temperature optima, indicating that the immobilization by this method imparted the structural and conformational stability to this enzyme. The immobilized enzyme also retained a high catalytic activity and showed the increased thermal stability compared with a free enzyme. By comparison of decimal reduction time values obtained at 50°C, it can be concluded that the immobilized enzyme was approximately 5-fold more stable than a free enzyme. The immobilization procedure developed is quite simple and easily reproduced, and provides a promising solution for the application of penicillin acylase for the purpose of 6-aminopenicillanic acid production.en
dc.publisherAssociation of the Chemical Engineers of Serbia
dc.relationProjekat Ministarstva nauke Republike Srbije, br. BTH1008
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceChemical Industry & Chemical Engineering Quarterly
dc.subjectpenicillin G acylaseen
dc.subjectcovalent immobilizationen
dc.subjectepoxy-activated polymethacrylic carrieren
dc.subjectsepabeads EC-EPen
dc.subjectthermal stabilityen
dc.titlePreparation and characterization of penicillin acylase immobilized on sepabeads EC-EP carrieren
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage210
dc.citation.issue4
dc.citation.other13(4): 205-210
dc.citation.spage205
dc.citation.volume13
dc.identifier.doi10.2298/CICEQ0704205Z
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/8572/1451-93720704205Z.pdf
dc.identifier.scopus2-s2.0-60249094573
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу