Show simple item record

dc.creatorPajić-Lijaković, Ivana
dc.creatorBugarski, Diana
dc.creatorPlavšić, Milenko B.
dc.creatorBugarski, Branko
dc.date.accessioned2021-03-10T10:43:07Z
dc.date.available2021-03-10T10:43:07Z
dc.date.issued2007
dc.identifier.issn1359-5113
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/1088
dc.description.abstractA mathematical model was formulated to describe hybridoma cell growth within the alginate-poly-L-lysine (alginate-PLL) microcapsules during air-lift bioreactor cultivation. Model development was based on experimentally obtained data concerning the hybridoma cell counts, monoclonal antibody (mAb) production and the distribution of hybridoma cell growth within the microcapsules. The cell growth was modeled using a mean field approach expressed as Langevin class of equations for two different regions of alginate-PLL microcapsules, the alginate microcapsule core and the annular region between microcapsule core and membrane. In this paper we propose an influence of microenvironmental conditions on cell growth. The osmotic pressure changes in the Na-alginate liquefied annular region, as well as, the resistance effects of Ca-alginate hydrogel in the core region during the cell growth were incorporated into the model. Good agreement between the experimental data and model prediction values was obtained. The proposed model successfully predicted the impact of various microenvironmental restriction effects on the dynamics of cell growth and appears useful for further optimization of microcapsule design in order to achieve higher intra-capsule cell concentrations resulting in higher amounts of mAb produced.en
dc.publisherElsevier Sci Ltd, Oxford
dc.rightsrestrictedAccess
dc.sourceProcess Biochemistry
dc.subjecthybridoma cell growthen
dc.subjectmicrocapsuleen
dc.subjectmodelingen
dc.subjectmicroenvironmental restrictionsen
dc.subjectmicrocapsule membrane effectsen
dc.subjectosmotic pressureen
dc.titleInfluence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsuleen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage174
dc.citation.issue2
dc.citation.other42(2): 167-174
dc.citation.rankaM21
dc.citation.spage167
dc.citation.volume42
dc.identifier.doi10.1016/j.procbio.2006.07.023
dc.identifier.rcubconv_2787
dc.identifier.scopus2-s2.0-33846277583
dc.identifier.wos000244233300006
dc.type.versionpublishedVersion


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record