A mathematical model was formulated to describe yeast cell growth within the Ca-alginate microbead during air-lift bioreactor cultivation. Model development was based on experimentally obtained data for the intra-bead cell concentration profile, after reached the equilibrium state, as well as, total yeast cell concentration per microbed and microbead volume as function of time. Relatively uniform cell concentration in the carrier matrix indicated that no internal nutrient diffusion limitations, but microenvironmental restriction, affected dominantly the dynamics of cell growth. Also interesting phenomenon of very different rates of cell number growth during cultivation is observed. After some critical time, the growth rate of cell colonies decreased drastically, but than suddenly increased again under all other experimental condition been the same. It is interpreted as disintegration of gel network and opening new free space for growth of cell clusters. These complex phenomena are mode...led using the thermodynamical, free energy formalism. The particular form of free energy functional is proposed to describe various kinds of interactions, which affected the dynamics of cell growth and cause pseudo-phase transition of hydrogel. The good agreement of experimentally obtained data and model predictions are obtained. In that way the model provides both, the quantitative tools for further technological optimization of the process and deeper insight into dynamics of cell growth mechanism.
TY - JOUR
AU - Pajić-Lijaković, Ivana
AU - Plavšić, Milenko B.
AU - Bugarski, Branko
AU - Nedović, Viktor
PY - 2007
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1091
AB - A mathematical model was formulated to describe yeast cell growth within the Ca-alginate microbead during air-lift bioreactor cultivation. Model development was based on experimentally obtained data for the intra-bead cell concentration profile, after reached the equilibrium state, as well as, total yeast cell concentration per microbed and microbead volume as function of time. Relatively uniform cell concentration in the carrier matrix indicated that no internal nutrient diffusion limitations, but microenvironmental restriction, affected dominantly the dynamics of cell growth. Also interesting phenomenon of very different rates of cell number growth during cultivation is observed. After some critical time, the growth rate of cell colonies decreased drastically, but than suddenly increased again under all other experimental condition been the same. It is interpreted as disintegration of gel network and opening new free space for growth of cell clusters. These complex phenomena are modeled using the thermodynamical, free energy formalism. The particular form of free energy functional is proposed to describe various kinds of interactions, which affected the dynamics of cell growth and cause pseudo-phase transition of hydrogel. The good agreement of experimentally obtained data and model predictions are obtained. In that way the model provides both, the quantitative tools for further technological optimization of the process and deeper insight into dynamics of cell growth mechanism.
PB - Elsevier, Amsterdam
T2 - Journal of Biotechnology
T1 - Ca-alginate hydrogel mechanical transformations - The influence on yeast cell growth dynamics
EP - 452
IS - 3
SP - 446
VL - 129
DO - 10.1016/j.jbiotec.2007.01.017
ER -
@article{
author = "Pajić-Lijaković, Ivana and Plavšić, Milenko B. and Bugarski, Branko and Nedović, Viktor",
year = "2007",
abstract = "A mathematical model was formulated to describe yeast cell growth within the Ca-alginate microbead during air-lift bioreactor cultivation. Model development was based on experimentally obtained data for the intra-bead cell concentration profile, after reached the equilibrium state, as well as, total yeast cell concentration per microbed and microbead volume as function of time. Relatively uniform cell concentration in the carrier matrix indicated that no internal nutrient diffusion limitations, but microenvironmental restriction, affected dominantly the dynamics of cell growth. Also interesting phenomenon of very different rates of cell number growth during cultivation is observed. After some critical time, the growth rate of cell colonies decreased drastically, but than suddenly increased again under all other experimental condition been the same. It is interpreted as disintegration of gel network and opening new free space for growth of cell clusters. These complex phenomena are modeled using the thermodynamical, free energy formalism. The particular form of free energy functional is proposed to describe various kinds of interactions, which affected the dynamics of cell growth and cause pseudo-phase transition of hydrogel. The good agreement of experimentally obtained data and model predictions are obtained. In that way the model provides both, the quantitative tools for further technological optimization of the process and deeper insight into dynamics of cell growth mechanism.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Biotechnology",
title = "Ca-alginate hydrogel mechanical transformations - The influence on yeast cell growth dynamics",
pages = "452-446",
number = "3",
volume = "129",
doi = "10.1016/j.jbiotec.2007.01.017"
}
Pajić-Lijaković, I., Plavšić, M. B., Bugarski, B.,& Nedović, V.. (2007). Ca-alginate hydrogel mechanical transformations - The influence on yeast cell growth dynamics. in Journal of Biotechnology
Elsevier, Amsterdam., 129(3), 446-452.
https://doi.org/10.1016/j.jbiotec.2007.01.017
Pajić-Lijaković I, Plavšić MB, Bugarski B, Nedović V. Ca-alginate hydrogel mechanical transformations - The influence on yeast cell growth dynamics. in Journal of Biotechnology. 2007;129(3):446-452.
doi:10.1016/j.jbiotec.2007.01.017 .
Pajić-Lijaković, Ivana, Plavšić, Milenko B., Bugarski, Branko, Nedović, Viktor, "Ca-alginate hydrogel mechanical transformations - The influence on yeast cell growth dynamics" in Journal of Biotechnology, 129, no. 3 (2007):446-452,
https://doi.org/10.1016/j.jbiotec.2007.01.017 . .
