Effect of fermentation conditions on lipase production by Candida utilis
Uticaj uslova fermentacije na produkciju lipaze iz Candida utilis
dc.creator | Grbavčić, Sanja | |
dc.creator | Dimitrijević-Branković, Suzana | |
dc.creator | Bezbradica, Dejan | |
dc.creator | Šiler-Marinković, Slavica | |
dc.creator | Knežević, Zorica | |
dc.date.accessioned | 2021-03-10T10:45:46Z | |
dc.date.available | 2021-03-10T10:45:46Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 0352-5139 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1129 | |
dc.description.abstract | A wild yeast strain isolated from spoiled soybean oil and identified as Candida utilis initially presented rather low lipase activity (approximately 4 IU dm-3) in submerged culture in a universal yeast medium containing 2 % malt extract. Studies were undertaken to improve the lipase production. The best yields of lipase were obtained with a medium supplemented with caprylic and oleic acids as inducers, but higher concentrations of the former ( gt 0.5 %) had a negative effect on the lipase production and cell growth. The type of nitrogen source seemed also to be very important. The highest lipolytic activity of 284 IU dm-3 was achieved after 5 days of fermentation in a medium containing oleic acid and hydrolyzed casein as carbon and nitrogen sources, respectively, and supplemented with Tween 80®. It was shown that optimization of the fermentation conditions can lead to a significant improvement in the lipase production (more than 70-fold higher compared to the initial value obtained in the non-optimized medium). . | en |
dc.description.abstract | U radu je ispitana mogućnost proizvodnje lipaze pomoću divljeg soja kvasca izolovanog iz pokvarenog sojinog ulja i identifikovanog kao Candida utilis. Ovaj mikroorganizam je po- kazao malu lipolitičku aktivnost kada je uzgajan u 2 % sladnom bujonu kao univerzalnom kvaščevom medijumu (oko 4 IU dm-3). Ispitani su uticaji različitih faktora kao što su sastav hranljive podloge i dodatak određenih induktora i stimulatora produkcije lipaza na povećanje lipolitičke aktivnosti. Oleinska i kaprilna kiselina su se pokazale kao najefikasniji induktori produkcije lipaze. U malim koncentracijama (do 0,5 %) kaprilna kiselina je pokazala veći uticaj na proizvodnju lipaze od oleinske kiseline, da bi pri većim koncentracijama imala toksičan uticaj na ćelijski rast. Proces je optimizovan i sa aspekta izvora azota. Najveća lipolitička aktivnost od 284 IU dm-3 ostvarena je nakon 5 dana fermentacije u optimizovanoj podlozi koja je sadržala oleinsku kiselinu i hidrolizat kazeina kao izvore ugljenika i azota i Tween 80® kao stimulator produkcije enzima. Pokazano je da se pažljivim izborom sastava hranljive podloge može značajno povećati produkcija lipaza (više od 70 puta u poređenju sa početnom vrednošću dobijenom u neoptimizovanoj podlozi). . | sr |
dc.publisher | Serbian Chemical Society, Belgrade | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of the Serbian Chemical Society | |
dc.subject | Candida utilis | en |
dc.subject | lipase production | en |
dc.subject | media optimization | en |
dc.subject | hydrolyzed casein | en |
dc.subject | oleic acid | en |
dc.title | Effect of fermentation conditions on lipase production by Candida utilis | en |
dc.title | Uticaj uslova fermentacije na produkciju lipaze iz Candida utilis | sr |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.epage | 765 | |
dc.citation.issue | 8-9 | |
dc.citation.other | 72(8-9): 757-765 | |
dc.citation.rank | M23 | |
dc.citation.spage | 757 | |
dc.citation.volume | 72 | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/13245/0352-51390709757G.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_technorep_1129 | |
dc.identifier.scopus | 2-s2.0-34548457813 | |
dc.identifier.wos | 000249768600003 | |
dc.type.version | publishedVersion |