The aim of this study was to examine the effect of hydrogels different chemical construction and synthesized under different conditions, to oxidative activity of rat peritoneal macrophages. The hydrogels are synthesized by radiation and chemical polymerization of 2-hydroxyethylmethacrylate (HEMA), copolymerization of HEMA with itaconic acid (IA), and copolymerization of HEMA, IA with poly(alkylene glycol) (met)acrylates-Bisomers. After incubation materials with macrophages, the effect of hydrogels was examine. All of tested materials reduct chemiluminescent activity of zimozan stimulated macrophages. Radiation polimerized PHEMA shows high inhibitory effect than chemical polimerized PHEMA. Hydrogels with more itaconic acid show high reducity effect, too. Different conditions of polimerization show differences in reducity effects to chemiluminescent activity of macrophages only in definite (order) reference (relation) between components of copolymers.
U radu je ispitivan efekat hidrogelova različitog hemijskog sastava i sintetisanih pod različitim uslovima na oksidativnu aktivnost peritonelanih makrofaga pacova. Hidrogelovi su dobijeni termički (hemijski) i radijaciono indukovanom polimerizacijom 2-hidroksietil metakrilata (HEMA) kopolimerizacijom HEMA i itakonske kiseline (IK), kao i kopolimerizacijom HEMA, IK i poli(alkilen glikol) (met)akrilata-Bisomeri (BIS). Efekat hidrogelova je ispitivan posle inkubacije materijala sa makrofagima dobijenim peritonealnom lavažom. Svi ispitivani materijali redukuju hemiluminescentnu aktivnost makrofaga stimulisanih zimozanom. Radijaciono umreženi PHEMA pokazuje veći inhibitorni efekat od hemijski umreženog. Hidrogelovi sa više itakonske kiseline pokazuju veći redukujući efekat. Različit način umrežavanja pokazuje razlike u redukujućim efektima na hemiluminescentnu aktivnost makrofaga samo pri određenim molarnim odnosima komponenti kopolimera.
Keywords:
hydrogels / HEMA / chemiluminescent test / citotoxity test / hidrogel / HEMA / hemiluminiscencija / makrofag / citotoksičnost
TY - JOUR
AU - Milojević, Mirjana
AU - Najman, Stevo
AU - Tomić, Simonida
PY - 2007
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1187
AB - The aim of this study was to examine the effect of hydrogels different chemical construction and synthesized under different conditions, to oxidative activity of rat peritoneal macrophages. The hydrogels are synthesized by radiation and chemical polymerization of 2-hydroxyethylmethacrylate (HEMA), copolymerization of HEMA with itaconic acid (IA), and copolymerization of HEMA, IA with poly(alkylene glycol) (met)acrylates-Bisomers. After incubation materials with macrophages, the effect of hydrogels was examine. All of tested materials reduct chemiluminescent activity of zimozan stimulated macrophages. Radiation polimerized PHEMA shows high inhibitory effect than chemical polimerized PHEMA. Hydrogels with more itaconic acid show high reducity effect, too. Different conditions of polimerization show differences in reducity effects to chemiluminescent activity of macrophages only in definite (order) reference (relation) between components of copolymers.
AB - U radu je ispitivan efekat hidrogelova različitog hemijskog sastava i sintetisanih pod različitim uslovima na oksidativnu aktivnost peritonelanih makrofaga pacova. Hidrogelovi su dobijeni termički (hemijski) i radijaciono indukovanom polimerizacijom 2-hidroksietil metakrilata (HEMA) kopolimerizacijom HEMA i itakonske kiseline (IK), kao i kopolimerizacijom HEMA, IK i poli(alkilen glikol) (met)akrilata-Bisomeri (BIS). Efekat hidrogelova je ispitivan posle inkubacije materijala sa makrofagima dobijenim peritonealnom lavažom. Svi ispitivani materijali redukuju hemiluminescentnu aktivnost makrofaga stimulisanih zimozanom. Radijaciono umreženi PHEMA pokazuje veći inhibitorni efekat od hemijski umreženog. Hidrogelovi sa više itakonske kiseline pokazuju veći redukujući efekat. Različit način umrežavanja pokazuje razlike u redukujućim efektima na hemiluminescentnu aktivnost makrofaga samo pri određenim molarnim odnosima komponenti kopolimera.
PB - Savez inženjera i tehničara Srbije, Beograd
T2 - Tehnika - Novi materijali
T1 - Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages
T1 - Efekat hidrogelova na bazi 2-hidroksietil metakrilata na hemiluminescentnu aktivnost peritonealnih makrofaga pacova
EP - 8
IS - 3
SP - 5
VL - 16
UR - https://hdl.handle.net/21.15107/rcub_technorep_1187
ER -
@article{
author = "Milojević, Mirjana and Najman, Stevo and Tomić, Simonida",
year = "2007",
abstract = "The aim of this study was to examine the effect of hydrogels different chemical construction and synthesized under different conditions, to oxidative activity of rat peritoneal macrophages. The hydrogels are synthesized by radiation and chemical polymerization of 2-hydroxyethylmethacrylate (HEMA), copolymerization of HEMA with itaconic acid (IA), and copolymerization of HEMA, IA with poly(alkylene glycol) (met)acrylates-Bisomers. After incubation materials with macrophages, the effect of hydrogels was examine. All of tested materials reduct chemiluminescent activity of zimozan stimulated macrophages. Radiation polimerized PHEMA shows high inhibitory effect than chemical polimerized PHEMA. Hydrogels with more itaconic acid show high reducity effect, too. Different conditions of polimerization show differences in reducity effects to chemiluminescent activity of macrophages only in definite (order) reference (relation) between components of copolymers., U radu je ispitivan efekat hidrogelova različitog hemijskog sastava i sintetisanih pod različitim uslovima na oksidativnu aktivnost peritonelanih makrofaga pacova. Hidrogelovi su dobijeni termički (hemijski) i radijaciono indukovanom polimerizacijom 2-hidroksietil metakrilata (HEMA) kopolimerizacijom HEMA i itakonske kiseline (IK), kao i kopolimerizacijom HEMA, IK i poli(alkilen glikol) (met)akrilata-Bisomeri (BIS). Efekat hidrogelova je ispitivan posle inkubacije materijala sa makrofagima dobijenim peritonealnom lavažom. Svi ispitivani materijali redukuju hemiluminescentnu aktivnost makrofaga stimulisanih zimozanom. Radijaciono umreženi PHEMA pokazuje veći inhibitorni efekat od hemijski umreženog. Hidrogelovi sa više itakonske kiseline pokazuju veći redukujući efekat. Različit način umrežavanja pokazuje razlike u redukujućim efektima na hemiluminescentnu aktivnost makrofaga samo pri određenim molarnim odnosima komponenti kopolimera.",
publisher = "Savez inženjera i tehničara Srbije, Beograd",
journal = "Tehnika - Novi materijali",
title = "Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages, Efekat hidrogelova na bazi 2-hidroksietil metakrilata na hemiluminescentnu aktivnost peritonealnih makrofaga pacova",
pages = "8-5",
number = "3",
volume = "16",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1187"
}
Milojević, M., Najman, S.,& Tomić, S.. (2007). Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages. in Tehnika - Novi materijali
Savez inženjera i tehničara Srbije, Beograd., 16(3), 5-8.
https://hdl.handle.net/21.15107/rcub_technorep_1187
Milojević M, Najman S, Tomić S. Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages. in Tehnika - Novi materijali. 2007;16(3):5-8.
https://hdl.handle.net/21.15107/rcub_technorep_1187 .
Milojević, Mirjana, Najman, Stevo, Tomić, Simonida, "Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages" in Tehnika - Novi materijali, 16, no. 3 (2007):5-8,
https://hdl.handle.net/21.15107/rcub_technorep_1187 .
