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Degradacija i karakterizacija pektina jabuke sorte budimka

dc.creatorNikolić, Miloš V.
dc.creatorMojović, Ljiljana
dc.date.accessioned2021-03-10T10:59:02Z
dc.date.available2021-03-10T10:59:02Z
dc.date.issued2008
dc.identifier.issn0352-5139
dc.identifier.urihttp://TechnoRep.tmf.bg.ac.rs/handle/123456789/1333
dc.description.abstractThe characterization of apple pectin and its oligogalacturonic fractions derived from the autochthones apple variety Budimka, characteristic for central Serbia, is described in this paper. After extraction, the apple pectin was subjected to controlled enzymatic hydrolysis by polygalacturonase (PG) and pectin lyase (PL) from Aspergillus niger and then fractionated by ion-exchange column chromatography on Dowex 1X-8 (200-400 mesh). Saturated oligo­galacturonic acids, obtained by controlled hydrolysis with PG, were efficiently separated by elution with a gradient of Na acetate buffer (pH 6.0), while unsaturated oligogalacturonic acids, obtained by controlled hydrolysis with PL, were separated on the same resin, using a gradient of Na formate buffer (pH 4.7) as the eluent. The yields of the fractions with the particular degree of polymerization (DP) were also determined. The total content of neutral saccharides in the original Budimka apple pectin was detected by HPLC analysis of the 4-nitrobenzoyl derivatives of the sugar, and amounted to 5.31%. Among the neutral saccharides, contents of galactose, glucose, rhamnose, arabinose, xylose and mannose were detected.en
dc.description.abstractU ovom radu je ispitivan hemijski sastav i karakteristike pektina jabuke autohtone sorte budimka, koja je karakteristična za region centralne Srbije. Nakon ekstrakcije pektina iz jabuke, izvršena je kontrolisana enzimska hidroliza pomoću enzima poligalakturonaze (PG) i pektinlijaze (PL) iz Aspergillus niger i dobijeni hidrolizati su frakcionisani pomoću jonoizmenjivačke kolone Dowex 1X-8. Separacija zasićenih oligogalakturonskih kiselina, dobijenih kontrolisanom hidrolizom pomoću poligalakturonaze je efikasno izvršena gradijentnom elucijom sa Na-acetatnim puferom, pH 6,0, dok je separacija nezasićenih oligogalakturonskih kiselina dobijenih kontrolisanom hidrolizom sa pektinlijazom izvršena na istoj jonoizmenjivačkoj koloni, ali korišćenjem Naformijatnog pufera pH 4,7. Takođe je utvrđen prinos individualnih frakcija različitog stepena polimerizacije. Ukupan sadržaj neutralnih saharida, koji je utvrđen pomoću HPLC je iznosio 5,31 %. Neutralne šećere pektina jabuke budimka čine ramnoza, arabinoza, ksiloza, manozaiglukoza.sr
dc.publisherSerbian Chemical Society, Belgrade
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceJournal of the Serbian Chemical Society
dc.subjectdegradationen
dc.subjectfractionationen
dc.subjectoligogalacturonic acidsen
dc.subjectBudimka apple pectinen
dc.subjectpolygalacturonaseen
dc.subjectpectin lyaseen
dc.subjectneutral saccharidesen
dc.titleCharacterization and degradation of pectin derived from Budimka appleen
dc.titleDegradacija i karakterizacija pektina jabuke sorte budimkasr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage167
dc.citation.issue2
dc.citation.other73(2): 157-167
dc.citation.rankM23
dc.citation.spage157
dc.citation.volume73
dc.identifier.fulltexthttp://TechnoRep.tmf.bg.ac.rs/bitstream/id/10816/0352-51390802157N.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_technorep_1333
dc.identifier.scopus2-s2.0-39449132430
dc.identifier.wos000258352000004
dc.type.versionpublishedVersion


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