Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid a...s a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.
TY - JOUR
AU - Lu, Xiyang
AU - Yao, Yao
AU - Yang, Yang
AU - Zhang, Zhongxi
AU - Gu, Jinjie
AU - Mojović, Ljiljana
AU - Knežević-Jugović, Zorica
AU - Baganz, Frank
AU - Lye, Gary
AU - Shi, Jiping
AU - Hao, Jian
PY - 2021
UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/14
AB - Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.
PB - Wiley, Hoboken
T2 - Biotechnology and Applied Biochemistry
T1 - Ethylene glycol and glycolic acid production by wild-typeEscherichia coli
EP - 755
IS - 4
SP - 744
SP - 755
VL - 68
DO - 10.1002/bab.1987
ER -
@article{
author = "Lu, Xiyang and Yao, Yao and Yang, Yang and Zhang, Zhongxi and Gu, Jinjie and Mojović, Ljiljana and Knežević-Jugović, Zorica and Baganz, Frank and Lye, Gary and Shi, Jiping and Hao, Jian",
year = "2021",
abstract = "Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology and Applied Biochemistry",
title = "Ethylene glycol and glycolic acid production by wild-typeEscherichia coli",
pages = "755-744-755",
number = "4",
volume = "68",
doi = "10.1002/bab.1987"
}
Lu, X., Yao, Y., Yang, Y., Zhang, Z., Gu, J., Mojović, L., Knežević-Jugović, Z., Baganz, F., Lye, G., Shi, J.,& Hao, J.. (2021). Ethylene glycol and glycolic acid production by wild-typeEscherichia coli. in Biotechnology and Applied Biochemistry
Wiley, Hoboken., 68(4), 744-755.
https://doi.org/10.1002/bab.1987
Lu X, Yao Y, Yang Y, Zhang Z, Gu J, Mojović L, Knežević-Jugović Z, Baganz F, Lye G, Shi J, Hao J. Ethylene glycol and glycolic acid production by wild-typeEscherichia coli. in Biotechnology and Applied Biochemistry. 2021;68(4):744-755.
doi:10.1002/bab.1987 .
Lu, Xiyang, Yao, Yao, Yang, Yang, Zhang, Zhongxi, Gu, Jinjie, Mojović, Ljiljana, Knežević-Jugović, Zorica, Baganz, Frank, Lye, Gary, Shi, Jiping, Hao, Jian, "Ethylene glycol and glycolic acid production by wild-typeEscherichia coli" in Biotechnology and Applied Biochemistry, 68, no. 4 (2021):744-755,
https://doi.org/10.1002/bab.1987 . .
