Lipases and proteases produced by indigenous Pseudomonas aeruginosa strain as potential detergent additives

Abstract

Enzymes produced by indigenous Pseudomonas aeruginosa strain have been subjected to research considering their potential application as detergent additives. As previously noted, lipase produced by Pseudomonas aeruginosa is highly alkaline, thermostable and solvent tolerant. Furthermore, same strain exhibits both lipase and protease activity establishing this lipase as potentially desirable component of enzyme-containing detergents. Further research was carried out to investigate insusceptibility of this lipase against coexisting native protease, several commercial surfactants, oxidizing agents and commercial detergents. Lipases and proteases remained highly active when incubated with several different surfactants and oxidizing agents under washing conditions. Moreover, presence of surfactants and oxidizing agents such as Tween® 20 and Triton® X-100 initially augment lipase and protease activity. Additionally, crude lipase preparation was insusceptible to coexisting native protease hence indicating possible storage stability. Overall, the remarkable properties of these enzymes make them potential detergent additives.

Enzimi iz ekstremofilne Pseudomonas aeruginosa vrste pokazuju visoku stabilnost u alkalnoj sredini što ih čini pogodnim za primenu u proizvodnji deterdženata. U ovom radu ispitana je aktivnost lipaza i proteaza dobijenih iz ove vrste u uslovima od značaja za industriju deterdženata. Ustanovljeno je da u realnim uslovima pranja, oba enzima zadržavaju visoku aktivnost u prisustvu različitih površinski aktivnih materija i oksidacionih agenasa. Takođe, prisustvo nekih aditiva kao što su Tween® 20, Triton® X-100, SDS i NaClO u početnoj fazi povećava lipolitičku aktivnost dok je povećanje proteolitičke aktivnosti primećeno u formulacijama koje sadrže Tween® 20 i Triton® X-100. Nadalje, sama lipaza je očuvala aktivnost u prisustvu nativnih proteaza što upućuje na zaključak da bi ovi enzimi zadržali visoku efikasnost u formulacijama deterdženata i tokom procesa pranja/čišćenja.