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Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability

Authorized Users Only
2009
Authors
Ognjanović, Nevena
Bezbradica, Dejan
Knežević-Jugović, Zorica
Article (Published version)
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Abstract
The feasibility of using the commercial immobilized lipase from Candida antarctica (Novozyme 435) to synthesize biodiesel from sunflower oil in a solvent-free system has been proved. Using methanol as an acyl acceptor and the response surface methodology as an optimization technique, the optimal conditions for the transesterification has been found to be: 45 degrees C, 3% of enzyme based on oil weight, 3:1 methanol to oil molar ratio and with no added water in the system. Under these conditions, gt 99% of oil conversion to fatty acid methyl ester (FAME) has been achieved after 50 h of reaction, but the activity of the immobilized lipase decreased markedly over the course of repeated runs. In order to improve the enzyme stability, several alternative acyl acceptors have been tested for biodiesel production under solvent-free conditions. The use of methyl acetate seems to be of great interest, resulting in high FAME yield (95.65%) and increasing the half-life of the immobilized lipase b...y about 20.1 times as compared to methanol. The reaction has also been verified in the industrially feasible reaction system including both a batch stirred tank reactor and a packed bed reactor. Although satisfactory performance in the batch stirred tank reactor has been achieved, the kinetics in a packed bed reactor system seems to have a slightly better profile (93.6 +/- 3.75% FAME yield after 8-10 h), corresponding to the volumetric productivity of 48.5 g/(dm(3) h). The packed bed reactor has operated for up to 72 h with almost no loss in productivity, implying that the proposed process and the immobilized system could provide a promising solution for the biodiesel synthesis at the industrial scale.

Keywords:
Candida antarctica lipase / Biodiesel / Transesterification / Methyl acetate / Packed bed reactor
Source:
Bioresource Technology, 2009, 100, 21, 5146-5154
Publisher:
  • Elsevier Sci Ltd, Oxford
Funding / projects:
  • Razvoj biotehnoloških postupaka za proizvodnju aditiva i novih formulacija za prehrambenu industriju (RS-20064)

DOI: 10.1016/j.biortech.2009.05.068

ISSN: 0960-8524

PubMed: 19540754

WoS: 000268742800033

Scopus: 2-s2.0-67650333363
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URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1431
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Ognjanović, Nevena
AU  - Bezbradica, Dejan
AU  - Knežević-Jugović, Zorica
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1431
AB  - The feasibility of using the commercial immobilized lipase from Candida antarctica (Novozyme 435) to synthesize biodiesel from sunflower oil in a solvent-free system has been proved. Using methanol as an acyl acceptor and the response surface methodology as an optimization technique, the optimal conditions for the transesterification has been found to be: 45 degrees C, 3% of enzyme based on oil weight, 3:1 methanol to oil molar ratio and with no added water in the system. Under these conditions,  gt 99% of oil conversion to fatty acid methyl ester (FAME) has been achieved after 50 h of reaction, but the activity of the immobilized lipase decreased markedly over the course of repeated runs. In order to improve the enzyme stability, several alternative acyl acceptors have been tested for biodiesel production under solvent-free conditions. The use of methyl acetate seems to be of great interest, resulting in high FAME yield (95.65%) and increasing the half-life of the immobilized lipase by about 20.1 times as compared to methanol. The reaction has also been verified in the industrially feasible reaction system including both a batch stirred tank reactor and a packed bed reactor. Although satisfactory performance in the batch stirred tank reactor has been achieved, the kinetics in a packed bed reactor system seems to have a slightly better profile (93.6 +/- 3.75% FAME yield after 8-10 h), corresponding to the volumetric productivity of 48.5 g/(dm(3) h). The packed bed reactor has operated for up to 72 h with almost no loss in productivity, implying that the proposed process and the immobilized system could provide a promising solution for the biodiesel synthesis at the industrial scale.
PB  - Elsevier Sci Ltd, Oxford
T2  - Bioresource Technology
T1  - Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability
EP  - 5154
IS  - 21
SP  - 5146
VL  - 100
DO  - 10.1016/j.biortech.2009.05.068
ER  - 
@article{
author = "Ognjanović, Nevena and Bezbradica, Dejan and Knežević-Jugović, Zorica",
year = "2009",
abstract = "The feasibility of using the commercial immobilized lipase from Candida antarctica (Novozyme 435) to synthesize biodiesel from sunflower oil in a solvent-free system has been proved. Using methanol as an acyl acceptor and the response surface methodology as an optimization technique, the optimal conditions for the transesterification has been found to be: 45 degrees C, 3% of enzyme based on oil weight, 3:1 methanol to oil molar ratio and with no added water in the system. Under these conditions,  gt 99% of oil conversion to fatty acid methyl ester (FAME) has been achieved after 50 h of reaction, but the activity of the immobilized lipase decreased markedly over the course of repeated runs. In order to improve the enzyme stability, several alternative acyl acceptors have been tested for biodiesel production under solvent-free conditions. The use of methyl acetate seems to be of great interest, resulting in high FAME yield (95.65%) and increasing the half-life of the immobilized lipase by about 20.1 times as compared to methanol. The reaction has also been verified in the industrially feasible reaction system including both a batch stirred tank reactor and a packed bed reactor. Although satisfactory performance in the batch stirred tank reactor has been achieved, the kinetics in a packed bed reactor system seems to have a slightly better profile (93.6 +/- 3.75% FAME yield after 8-10 h), corresponding to the volumetric productivity of 48.5 g/(dm(3) h). The packed bed reactor has operated for up to 72 h with almost no loss in productivity, implying that the proposed process and the immobilized system could provide a promising solution for the biodiesel synthesis at the industrial scale.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Bioresource Technology",
title = "Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability",
pages = "5154-5146",
number = "21",
volume = "100",
doi = "10.1016/j.biortech.2009.05.068"
}
Ognjanović, N., Bezbradica, D.,& Knežević-Jugović, Z.. (2009). Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability. in Bioresource Technology
Elsevier Sci Ltd, Oxford., 100(21), 5146-5154.
https://doi.org/10.1016/j.biortech.2009.05.068
Ognjanović N, Bezbradica D, Knežević-Jugović Z. Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability. in Bioresource Technology. 2009;100(21):5146-5154.
doi:10.1016/j.biortech.2009.05.068 .
Ognjanović, Nevena, Bezbradica, Dejan, Knežević-Jugović, Zorica, "Enzymatic conversion of sunflower oil to biodiesel in a solvent-free system: Process optimization and the immobilized system stability" in Bioresource Technology, 100, no. 21 (2009):5146-5154,
https://doi.org/10.1016/j.biortech.2009.05.068 . .

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