TechnoRep - Faculty of Technology and Metallurgy Repository
University of Belgrade - Faculty of Technology and Metallurgy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   TechnoRep
  • Tehnološko-metalurški fakultet
  • Radovi istraživača / Researchers’ publications (TMF)
  • View Item
  •   TechnoRep
  • Tehnološko-metalurški fakultet
  • Radovi istraživača / Researchers’ publications (TMF)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

A novel bioreactor with mechanical stimulation for skeletal tissue engineering

Thumbnail
2009
1451-93720901041P.pdf (610.2Kb)
Authors
Petrović, Miloš
Mitraković, Dragan
Bugarski, Branko
Vonwil, Daniel
Martin, Ivan
Obradović, Bojana
Article (Published version)
Metadata
Show full item record
Abstract
The provision of mechanical stimulation is believed to be necessary for the functional assembly of skeletal tissues, which are normally exposed to a variety of biomechanical signals in vivo. In this paper, we present a development and validation of a novel bioreactor aimed for skeletal tissue engineering that provides dynamic compression and perfusion of cultivated tissues. Dynamic compression can be applied at frequencies up to 67.5 Hz and displacements down to 5 µm thus suitable for the simulation of physiological conditions in a native cartilage tissue (0.1-1 Hz, 5-10 % strain). The bioreactor also includes a load sensor that was calibrated so to measure average loads imposed on tissue samples. Regimes of the mechanical stimulation and acquisition of load sensor outputs are directed by an automatic control system using applications developed within the LabView platform. In addition, perfusion of tissue samples at physiological velocities (10-100 µm/s) provides efficient mass transfe...r, as well as the possibilities to expose the cells to hydrodynamic shear and simulate the conditions in a native bone tissue. Thus, the novel bioreactor is suited for studies of the effects of different biomechanical signals on in vitro regeneration of skeletal tissues, as well as for the studies of newly formulated biomaterials and cell biomaterial interactions under in vivo-like settings.

Keywords:
bioreactor / dynamic compression / perfusion / cartilage / bone / tissue engineering
Source:
Chemical Industry & Chemical Engineering Quarterly, 2009, 15, 1, 41-44
Publisher:
  • Association of the Chemical Engineers of Serbia
Funding / projects:
  • Swiss National Science FoundationSwiss National Science Foundation (SNSF) [IB73B0-111016/1]
  • Interakcija imobilisanih ćelija, tkiva i biološki aktivnih molekula u bioreaktorskim sistemima (RS-142075)

DOI: 10.2298/CICEQ0901041P

ISSN: 1451-9372

WoS: 000263780100011

Scopus: 2-s2.0-74049103091
[ Google Scholar ]
20
20
URI
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1453
Collections
  • Radovi istraživača / Researchers’ publications (TMF)
Institution/Community
Tehnološko-metalurški fakultet
TY  - JOUR
AU  - Petrović, Miloš
AU  - Mitraković, Dragan
AU  - Bugarski, Branko
AU  - Vonwil, Daniel
AU  - Martin, Ivan
AU  - Obradović, Bojana
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1453
AB  - The provision of mechanical stimulation is believed to be necessary for the functional assembly of skeletal tissues, which are normally exposed to a variety of biomechanical signals in vivo. In this paper, we present a development and validation of a novel bioreactor aimed for skeletal tissue engineering that provides dynamic compression and perfusion of cultivated tissues. Dynamic compression can be applied at frequencies up to 67.5 Hz and displacements down to 5 µm thus suitable for the simulation of physiological conditions in a native cartilage tissue (0.1-1 Hz, 5-10 % strain). The bioreactor also includes a load sensor that was calibrated so to measure average loads imposed on tissue samples. Regimes of the mechanical stimulation and acquisition of load sensor outputs are directed by an automatic control system using applications developed within the LabView platform. In addition, perfusion of tissue samples at physiological velocities (10-100 µm/s) provides efficient mass transfer, as well as the possibilities to expose the cells to hydrodynamic shear and simulate the conditions in a native bone tissue. Thus, the novel bioreactor is suited for studies of the effects of different biomechanical signals on in vitro regeneration of skeletal tissues, as well as for the studies of newly formulated biomaterials and cell biomaterial interactions under in vivo-like settings.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - A novel bioreactor with mechanical stimulation for skeletal tissue engineering
EP  - 44
IS  - 1
SP  - 41
VL  - 15
DO  - 10.2298/CICEQ0901041P
ER  - 
@article{
author = "Petrović, Miloš and Mitraković, Dragan and Bugarski, Branko and Vonwil, Daniel and Martin, Ivan and Obradović, Bojana",
year = "2009",
abstract = "The provision of mechanical stimulation is believed to be necessary for the functional assembly of skeletal tissues, which are normally exposed to a variety of biomechanical signals in vivo. In this paper, we present a development and validation of a novel bioreactor aimed for skeletal tissue engineering that provides dynamic compression and perfusion of cultivated tissues. Dynamic compression can be applied at frequencies up to 67.5 Hz and displacements down to 5 µm thus suitable for the simulation of physiological conditions in a native cartilage tissue (0.1-1 Hz, 5-10 % strain). The bioreactor also includes a load sensor that was calibrated so to measure average loads imposed on tissue samples. Regimes of the mechanical stimulation and acquisition of load sensor outputs are directed by an automatic control system using applications developed within the LabView platform. In addition, perfusion of tissue samples at physiological velocities (10-100 µm/s) provides efficient mass transfer, as well as the possibilities to expose the cells to hydrodynamic shear and simulate the conditions in a native bone tissue. Thus, the novel bioreactor is suited for studies of the effects of different biomechanical signals on in vitro regeneration of skeletal tissues, as well as for the studies of newly formulated biomaterials and cell biomaterial interactions under in vivo-like settings.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "A novel bioreactor with mechanical stimulation for skeletal tissue engineering",
pages = "44-41",
number = "1",
volume = "15",
doi = "10.2298/CICEQ0901041P"
}
Petrović, M., Mitraković, D., Bugarski, B., Vonwil, D., Martin, I.,& Obradović, B.. (2009). A novel bioreactor with mechanical stimulation for skeletal tissue engineering. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 15(1), 41-44.
https://doi.org/10.2298/CICEQ0901041P
Petrović M, Mitraković D, Bugarski B, Vonwil D, Martin I, Obradović B. A novel bioreactor with mechanical stimulation for skeletal tissue engineering. in Chemical Industry & Chemical Engineering Quarterly. 2009;15(1):41-44.
doi:10.2298/CICEQ0901041P .
Petrović, Miloš, Mitraković, Dragan, Bugarski, Branko, Vonwil, Daniel, Martin, Ivan, Obradović, Bojana, "A novel bioreactor with mechanical stimulation for skeletal tissue engineering" in Chemical Industry & Chemical Engineering Quarterly, 15, no. 1 (2009):41-44,
https://doi.org/10.2298/CICEQ0901041P . .

DSpace software copyright © 2002-2015  DuraSpace
About TechnoRep | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About TechnoRep | Send Feedback

OpenAIRERCUB